The production of poly(3-hydroxybutyrate) by thermophilic Caldimonas manganoxidans from glycerol

Springer Science and Business Media LLC - Tập 25 - Trang 1-8 - 2018
Li-Jung Hsiao1, Ming-Chieh Lee2, Po-Jen Chuang2, Yu-Yuan Kuo2, Ji-Hong Lin2, Tzong-Ming Wu3, Si-Yu Li2
1Department of Chemical Engineering, National Chung-Hsing University, Taichung, Taiwan
2Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan
3Department of Materials Science and Engineering, National Chung Hsing University, Taichung, Taiwan

Tóm tắt

Glycerol is an abundant and inexpensive raw material, suitable for the use in fermentation as value adding to agricultural waste. To fully understand how to convert both pure and refined glycerol into Poly(3-hydroxybutyrate) (PHB) using thermophilic Caldimonas manganoxidans. A high PHB concentration can be obtained by using the following fermentation conditions: un-buffered initial pH of 7 and 50 °C. The preset fermentation conditions with initial glycerol concentration of 50 g/L yielded a PHB concentration of 8.4 ± 1.5 g/L, PHB content of 71 ± 7 wt%, and an average molecular weight of 123 kDa. The average molecular weight of PHB as produced can be controlled by the initial glycerol concentration whereas a trade-off between the initial glycerol concentration and the average molecular weight was reported. The feasibility of using refined glycerol as carbon source for PHB production has been demonstrated. It is tunable to produce different Mw of PHB using glycerol, while biodiesel-derived glycerol can be used as carbon source.

Tài liệu tham khảo

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