Occurrence and molecular characteristics of citrus leaf blotch virus from citrus in China based on coat protein genes
Tóm tắt
A total of 791 citrus samples were collected from 10 citrus cultivars from nine different citrus-producing areas in China, the occurrence of citrus leaf blotch virus (CLBV) was detected by RT-PCR, and the coat protein (CP) genes of CLBV were amplified to analyze the variability of the CP genes. Results showed that in China, the incidence of CLBV was 7.7%, while CLBV was found in all sample locations and detected positive in 9 out of 10 citrus cultivars, indicating that CLBV is common in China. The CLBV isolates showed low diversity in their CP genes, and phylogenetic analysis revealed that CLBV isolates in this study were separated into different branches, indicating no clear relationship between their variation and citrus cultivar or geographic origin. The findings from this study provide important information for further research towards the population structure and epidemiology of CLBV.
Tài liệu tham khảo
Adams MJ, Candresse T, Hammond J, Kreuze JF, Martelli GP, Namba S, Pearson MN, Ryu KH, Saldarelli P, Yoshikawa N (2012) Family Betaflexiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus Taxonomy-Ninth Report on the International Committee on Taxonomy of Viruses. Elsevier Academic Press, London, pp 920–941
Cao MJ, Yu YQ, Tian X, Yang FY, Li RH, Zhou CY (2017) First report of Citrus leaf blotch virus in lemon in China. Plant Disease 101:1561
Chavan RR, Blouin AG, Cohen D, Pearson MN (2013) Characterization of the complete genome of a novel citrivirus infecting Actinidia chinensis. Archives of Virology 158:1679–1686
Dang T, Huang A, Bodaghi S, Tan S, Tian T, Posis K, McGuire B, McRoberts N, Vidalakis G (2020) First report of citrus leaf blotch virus infecting Bearss Lime Tree in California. Plant Disease 104:3088
Galipienso L, Navarro L, Ballester-Olmos JF, Pina J, Moreno P, Guerri J (2000) Host range and symptomatology of graft-transmissible pathogen causing bud union crease of citrus on trifoliate rootstocks. Plant Pathology 49:308–314
Guardo M, Sorrentino G, Marletta T, Caruso A (2007) First report of Citrus leaf blotch virus on Kumquat in Italy. Plant Disease 91:1054–1054
Hajeri S, Ramadugu C, Keremane M, Vidalakis G, Lee R (2010) Nucleotide sequence and genome organization of Dweet mottle virus and its relationship to members of the family Betaflexiviridae. Archives of Virology 155:1523–1527
Harper SJ, Chooi KM, Pearson MN (2008) First report of Citrus leaf blotch virus in New Zealand. Plant Disease 92:1470
Hernández-Rodríguez L, Pérez-Castro JM, García-García G, Ramos-González PL, Zamora-Rodríguez V, FerriolMarchena X, Peña-Bárzaga I, Riverend BL (2016) Citrus leaf blotch virus in Cuba: First report and partial molecular characterization. Tropical Plant Pathology 41:147–154
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35:1547–1549
Li P, Li M, Zhang S, Wang J, Yang FY, Cao MJ, Li ZA (2018) Complete genome sequences of four isolates of Citrus leaf blotch virus from citrus in China. Journal of Integrative Agriculture 17:712–715
Park CY, Park J, Kim H, Yi SI, Moon JS (2019) First report of Citrus leaf blotch virus in Satsuma mandarin in Korea. Journal of Plant Pathology 101:1229
Vives MC, Galipienso L, Navarro L, Moreno P, Guerri J (2001) The nucleotide sequence and genomic organization of Citrus leaf blotch virus: candidate type species for a new virus genus. Virology 287:225–233
Vives MC, Galipienso L, Navarro L, Moreno P, Guerri J (2002a) Characterization of two kinds of subgenomic RNAs produced by Citrus leaf blotch virus. Virology 295:328–336
Vives MC, Rubio L, Galipienso L, Navarro L, Moreno P, Guerri J (2002b) Low genetic variation between isolates of Citrus leaf blotch virus from different host species and different geographical origins. Journal of General Virology 83:2587–2591
Wang J, Zhu D, Tan Y, Zong X, Wei H, Liu Q (2016) First report of citrus leaf blotch virus in sweet cherry. Plant Disease 100:1027–1027
Xiang Z, Cheng Q, Xie ZZ, Wang GP, Ni H (2017) Incidence and molecular characteristics of citrus leaf blotch virus and citrus tatter leaf virus infecting citrus in China. Acta Hortic Sin 44:113–119 (In Chinese)
Xuan Z, Xie J, Yu H, Zhang S, Li RH, Cao MJ (2020) Mulberry (Morus alba) is a new natural host of Citrus leaf blotch virus in China. Plant Disease 105