Garlic ensures better protection than isolated diallyl sulfide against asbestos induced genotoxicity: an in vivo study

Springer Science and Business Media LLC - Tập 14 - Trang 245-252 - 2022
Mahvish Khan1, Mohtashim Lohani2,3, Saif Khan4, Saheem Ahmad5, Subuhi Sherwani1, Suresh Babu Jandrajupalli6, Freah Alshammary7, Nizar Ahmad Khamjan8, Farrukh Aqil9, Qamar Rahman10
1Department of Biology, College of Science, University of Ha’il, Ha’il, Saudi Arabia
2Department of Emergency Medical Services, Faculty of Applied Medical Sciences, College of Applied Medical Sciences, Jazan University, Jazan, Kingdom of Saudi Arabia
3Medical research Center, College of Applied Medical Sciences, Jazan University, Jazan, Saudi Arabia
4Department of Basic Dental and Medical Sciences, College of Dentistry, Ha’il University, Ha’il, Saudi Arabia
5Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Hail, Ha’il, Saudi Arabia
6Department of Preventive Dentistry, College of Dentistry, University of Ha’il, Ha’il, Saudi Arabia
7Department of Preventive Dental Sciences, College of Dentistry, Ha’il University, Ha’il, Saudi Arabia
8Department of Medical Laboratory Technology, College of Applied Medical Sciences, Jazan University, Jazan, Saudi Arabia
9Department of Medicine and James Graham Brown Cancer Center, University of Louisville, Louisville, USA
10Department of Biotechnology, Amity University, Lucknow, India

Tóm tắt

Garlic is an established antioxidant, owing its antioxidant property to a number of constituent sulfhydryl compounds, the most important of which is diallyl sulfide (DAS). Garlic and its constituents are known to protect against reactive oxygen species mediated damages by various toxicants including asbestos, a well known carcinogen. Present study is a first attempt to compare potential of crude garlic extract and DAS to protect against asbestos induced genotoxicity in vivo. Female albino rats were exposed to Indian chrysotile asbestos intratracheally. Other groups of rats were exposed to chrysotile and either garlic or diallyl sulfide. We employed the micronucleus and chromosomal aberrations assays in the alveolar macrophages to analyze the genotoxicity of asbestos fibers. Our results indicate that both garlic extract as well as DAS, when administered to rats, significantly reduced genetic damages induced by asbestos, although the protection by garlic extract was much higher than that of DAS. We conclude two important outcomes; first, garlic extract provides more robust protection as compared to any of its isolated constituents and second, the macrophages can be used as a non-invasive tool for genotoxicity assessment after exposure through inhalation.

Tài liệu tham khảo

Abidi P, Afaq F, Jamal MA, Lohani M, Rahman Q (1999) Chrysotile-mediated imbalance in the glutathione redox system in the development of pulmonary injury. Toxicol Lett 106:1–39 Acharya M, Lau-Cam CA (2010) Comparison of the protective actions of N-acetylcysteine, hypotaurine and taurine against acetaminophen-induced hepatotoxicity in the rat. J Biomed Sci 17(Suppl 1):S35. https://doi.org/10.1186/1423-0127-17-S1-S35 Afaq F, Abidi P, Matin R, Rahman Q (1998) Activation of alveolar macrophages and peripheral red blood cells in rats exposed to fiber/particles. Toxicol Lett 99:175–182 Ameen M, Musthapa S, Ahmad I, Ansari FA, Baig MA (2003) Alteration in cellular and biochemical markers of pulmonary toxicity in rat lung exposed to carpet dusts. Inhal Toxicol 15:1119–1131 Arif JM, Khan SG, Ahmad I, Joshi LD, Rahman Q (1997) Effect of kerosene and its soot on the chrysotile-mediated toxicity to the rat alveolar macrophages. Environ Res 72:232–238 Au WW, Bibbins P, Ward JB, Legator MS (1988) Development of a rodent lung macrophage aberration assay. Mutat Res 208:1–7 Balamayooran G, Batra S, Michael B, Kyle F, Happel I, Jeyaseelan S (2010) Mechanisms of neutrophil accumulation in the lungs against bacteria. Am J Respir Cell Mol Biol 43(1):5–16. https://doi.org/10.1165/rcmb.2009-0047TR Bhattacharya K, Yadava S, Papp T, Schiffmann D, Rahman Q (2004) Reduction of chrysotile asbestos-induced genotoxicity in human peripheral blood lymphocytes by garlic extract. Toxicol Lett 153(3):327–332 Bonham CA, Strek ME, Patterson KC (2016) From granuloma to fibrosis: sarcoidosis associated pulmonary fibrosis. Curr Opin Pulm Med 22(5):484–491. https://doi.org/10.1097/MCP.0000000000000301 Brain JD (1970) Free cells in the lung: some aspects of their role, quantitation and regulation. Arch Inter Med 126:477–487 Brody AR, Liu J-Y, Brass D (1997) Analyzing the genes and peptide growth factors expressed in lung cells in vivo consequent to asbestos exposure and in vivo. Environ Health Perspect 105:1165–1171 Limoli CL, Giedzinski E (2003) Induction of chromosomal instability by chronic oxidative stress. Neoplasia 5(4):339–346. https://doi.org/10.1016/S1476-5586(03)80027-1 Chun HS, Kim HJ, Choi EH (2001) Modulation of cytochrome p450 mediated bioactivation of benzo[o]pyrene by volatile allyl sulfides in human hepatoma cells. Biosci BiotechnolBiochem 65(10):2205–2212 Conner MK, Alarie Y et al (1979) Sister chromatid exchanges in murine alveolar macrophages, regenerating liver and bone marrow cells- a simultaneous multicellular in vivo assay. Chromosoma 74:41–55 Das RK, Sahu K, Dash BC (1994) Induction of chromosome aberrations and micronuclei in pulmonary alveolar macrophages of rats following inhalation of mosquito coil smoke. Mutat Res 320:285–292 Dopp E, Saedler J, Stopper H, Weiss DG, Schiffmann D (1995) Mitotic disturbances and micronucleus induction in Syrian hamster embryo fibroblast cells caused by asbestos fibers. Environ Health Perspect 103(3):268–271 Dopp E, Schuler M, Schiffmann D, Eastmond DA (1997) Induction of micronuclei and chromosomal breakage affecting the centric/ pericentric regions of chromosome 1 and 9 in human amniotic fluid cells after treatment with asbestos ceramic fibers. Mutat Res 337(1):77–87 Driscoll KE (1996) Effects of fibres on cell proliferation, cell activation and gene expression. IARC Sci Publ 140:73–96 Emerit I, Jaurand MC, Saint-Etienne L, Levy F (1991) Formation of a clastogenic factor by asbestos-treated rat pleural mesothelial cells. Agents Act 34:410–415 Evans MJ, Cabral RJ, Stephens RJ, Freeman G (1973) Cell division of alveolar macrophages in the rat lung following exposure to NO (2). Am J Pathol 70:199–207 Fenech M (1993) The cytokinesis blocks micronucleus technique. A detailed description on the method and its application to genotoxicity studies in human Population. Mutat Res 285:35–44 Gong P, Hu B, Cederbaum AI (2004) Diallyl sulfide induces heme oxygenase-1 through MAPK pathway. Arch Biochem Biophys 432(2):252–260 Guyonnet D, Seiss MH, Le Bon AM, Suschetet M (1999) Modulation of phase II enzymes by Organosulphur compounds from allium vegetables in rats tissues. Toxicol Appl Pharmacol 154:50–58 Hansteen IL, Hilt B, Lein JT, Skaug V, Haugen A (1993) Karyotypic changes in the preclinical and subsequent stages of maglinant mesothelioma: a case report. Cancer Genet Cytogenet 70:94–98 Hong JY, Wang ZY, Smith TJ, Zhou S, Shi S, Pau J, Yang CS (1992) Inhibitory effect of diallyl sulphide on the metabolism and tumorigenecity of tobacco-specific carcinogen 4-(methyl nitrosamine)-1-(3-pyridyl)-1-(butyanol (NNK) in A/J mouse lung. Carcinogenesis 13:901–904 Kamp DW, Weitzman SA (1999) The molecular basis of asbestos induced lung injury. Thorax 54(7):638–652 Klebe S, Leigh J, Henderson DW, Nurminen M (2020) Smoking and lung cancer: an update. Int J Environ Res Public Health. 17(1):258. https://doi.org/10.3390/ijerph17010258 Khan SG, Ali S, Rahman Q (1990) Protective role of ascorbic acid against asbestos induced toxicity in rat lungs: in vitro study. Drug Chem Toxicol 13:249–256 Knowles LM, Milner JA (2001) Possible mechanism by allyl sulfides suppress neoplastic cell proliferation. J Nutr 131(3s):1061S-1066S Kumagai-Takei N, Lee S, Srinivas B, Shimizu Y, Sada N, Yoshitome K, Ito T, Nishimura Y, Otsuki T (2020) The effects of asbestos fibers on human T cells. Int J Mol Sci 21(19):6987. https://doi.org/10.3390/ijms21196987 Kumarguru BN, Natarajan M, Dayananda S, Raghupathi AR et al (2015) Giant cell lesions of lungs: a histopathological and morphometric study of seven autopsy cases. J Clin Diagn Res 9(11):EC12–EC16. https://doi.org/10.7860/JCDR/2015/15035.6786 Leigh J, Bonin A, Wang H (2000) In vivo genotoxicity of crystalline silica as evidenced by micronuclie in pulmonary alveolar macrophages: low-dose study. Inhal Toxicol 12:199–204 Lohani M, Yadav S, Schiffmann D, Rahman Q (2003) Diallylsulfide attenuates asbestos-induced genotoxicity. Toxicol Lett 143(1):45–50 La Maestra S, Micale RT, Ferretti M, Izzotti A, Gaggero L (2020) Attenuation of oxidative stress and chromosomal aberrations in cultured macrophages and pulmonary cells following self-sustained high temperature synthesis of asbestos. Sci Reports. https://doi.org/10.1038/s41598-020-65620-x Oberdorster G (1994) Macrophage-associated response to chrysotile. Ann Occup Hyg 38:601–615 Oberdorster G (1997) Pulmonary carcinogenicity of inhaled particles and the maximum tolerated dose. Environ Health Perspect 105:1347–1355 Omiecinski CJ, Vanden Heuvel JP, Perdew GH, Peters JM (2011) Xenobiotic metabolism, disposition, and regulation by receptors: from biochemical phenomenon to predictors of major toxicities. Toxicol Sci 120(Supplement 1):S49–S75. https://doi.org/10.1093/toxsci/kfq338 Petruska JM, Wong SHY, Sunderman FW Jr, Mossman BT (1990) Detection of lipid peroxidation in lung and in broncoalveolar lavage cels and fluid. Free Rad Biol Med 9:51–58 Pinto JT, Rivlin RS (2001) Antiproliferation effects of allium derivatives from garlic. J Nutr 131(3s):1058S-1060S Prieditis H, Adamson IY (1996) Alveolar macrophage kinetics and multinucleated giant cell formation after lung injury. J Leukoc Biol 59(4):534–538 Quinlan TR, Berube KA, Marsh JP (1995) Patterns of inflammation, cell proliferation and related gene expression in lung cells after inhalation of chrysotile asbestos. Am J Pathol 147:728–739 Rahman Q, Dopp E, Lohani M, Schiffmann D (2000) Occupational and environmental factors enhancing the genotoxicity of asbestos. Inhal Toxico l 12(Suppl 3):157–165. https://doi.org/10.1080/08958378.2000.11463209 Reddy BS, Rao CV, Rivenson A, Kelloff G (1993) Chemoprevention of colon carcinogenesis by Organosulphur compounds. Cancer Res 53:3493–3498 Rithidech K, Chen BT, Mauderly JL, Whorton EB Jr, Brooks AL (1989) Cytogenetic effects of cigarette smoke on pulmonary alveolar macrophages of the rat. Environ Mol Mutagen 14(1):27–33 Sahu K, Das RK (1995) Micronucleus assay in pulmonary alveolar macrophages, a simple model to detect genotoxicity of environmental agents entering through the inhalation route. Mutat Res 347:61–65 Satyal P, Craft JD, Dosoky NS, Setzer WN (2017) The chemical compositions of the volatile oils of garlic (Allium sativum) and wild garlic (Allium vineale). Foods 6(8):63 Scott MJ, Ward JB Jr. Cytogenetic analysis of animals after subchronic inhalation exposure to formaldehyde: preliminary report of result from Sparague-Dawby rats in Texas Indoor quality study. Toxicology studies, University of Texas Health Science centre, School of public Health, Houston. Shrivastava SK, Hu X, Xia H, Zaren HA, Chatterjee ML, Agrawal R, Singh SV (1997) Mechanism of different efficacy of garlic organosulfides in preventing benzo[o] pyrene induced cancer in mice. Cancer Lett 118:61–67 Sumiyoshi H, Wargovich MJ (1990) Chemoprevention of 1,2-dimethylhydrazine-induced colon cancer in mice by natural occurring Organosulphur compounds. Cancer Res 50:5084–5087 Trout KL, Holian A (2020) Multinucleated giant cell phenotype in response to stimulation. Immunobiology 225(3):151952. https://doi.org/10.1016/j.imbio.2020.151952 White HJ, Garg BD (1981) Early pulmonary response of the rat lung to inhalation of high concentration of diesel particles. J Appl Toxicol 1:104–110 WHO (1986) Environmental health criteria 53. Asbestos and other mineral fibers. World Health Organization, Geneva Yang CS, Chhabra SK, Hong JY, Smith TJ (2001) Mechanisms of inhibition of chemical toxicity and carcinogenesis by diallyl sulphide (DAS) and vrelated compounds from garlic. J Nutr 131(3s):1041s-1045S Wang Y, Huo T, Feng C, Zeng Y, Jie Yang X, Zhang FD, Deng J (2019) Chrysotile asbestos induces apoptosis via activation of the p53-regulated mitochondrial pathway mediated by ROS in A549 cells. Appl Clay Sci. 182:105245 Zaidi SH (1969) Experimental pneumoconiosis. John Hopkins Press, Baltimore