Transcriptional Regulation of β-Glucanase Activity in the Ruminal Bacterium, Prevotella bryantii B14
Tóm tắt
The ruminal bacterium, Prevotella bryantii B14, grew more rapidly with glucose as an energy source than mannose (0.73 versus 0.47 h−1) and had 8-fold less β-glucanase activity (50 versus 400 nmol reducing sugar mg protein−1 min−1). Cultures that were provided with glucose and mannose had little β-glucanase activity even though both sugars were utilized simultaneously. The observation that glucose and mannose were utilized simultaneously indicated that β-glucanase expression was not merely a simple induction or inducer exclusion. When glucose was added to cultures growing on mannose, hexose flux through the glucomannokinase increased 1.5-fold, and this increase was associated with an almost immediate decrease in β-glucanase mRNA. After only three generation (doubling) times, the amount of β-glucanase mRNA was comparable to that observed in cells growing only with glucose. These results indicate that β-glucanase activity is transcriptionally regulated. However, further work will be needed to define more precisely the nature of this regulation and to identify the intermediate in this response.
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