Appearance of the terminal senescent cell population in human diploid fibroblasts analyzed by flow cytometry

Hayflick, 1961, The serial cultivation of human diploid cells strains, Exp. Cell Res., 25, 585, 10.1016/0014-4827(61)90192-6 Hayflick, 1965, The limited in vitro lifetime of human diploid cell strains, Exp. Cell. Res., 37, 614, 10.1016/0014-4827(65)90211-9 Orgel, 1973, Aging of clones of mammalian cells, Nature, 243, 441, 10.1038/243441a0 Schneider, 1975, Alteration in cellular RNAs during the in vitro lifespan of cultured human diploid fibroblasts, Cell, 6, 179, 10.1016/0092-8674(75)90008-2 Macieira-Coelho, 1966, The division cycle and RNA-synthesis in diploid human cells at different passage levels in vitro, Exp. Cell Res., 42, 673, 10.1016/0014-4827(66)90280-1 Razin, 1977, Comparison by autoradiography of macromolecular biosynthesis in “young” and “old” human diploid fibroblast cultures, 6, 379 Sahaisrivastava, 1973, Changes in enzymic activity during cultivation of human cells in vitro, Exp. Cell Res., 80, 305, 10.1016/0014-4827(73)90301-7 Ryan, 1975, Chromatin template activity during aging in WI38 cells, Exp. Cell. Res., 90, 456, 10.1016/0014-4827(75)90340-7 Schneider, 1975, Alteration in cellular RNAs during the in vitro lifespan of cultured human diploid fibroblasts, 4, 449 Hill, 1978, Evidence that transcription changes in ageing cultures are terminal events occurring after the expression of a reduced replicative potential, Mech. Ageing, Dev., 8, 85, 10.1016/0047-6374(78)90010-6 Bowman, 1976, Decreased synthesis of nucleolar RNA in aging human cells in vitro, Exp. Cell Res., 101, 434, 10.1016/0014-4827(76)90399-2 Crissman, 1975, Methods and applications of flow systems for analysis and sorting of mammalian cells, Vol. 9, 179 Crissman, 1973, Rapid simultaneous measurement of DNA, protein and cell volume in single cells from large mammalian cell populations, J. Cell. Biol., 59, 766, 10.1083/jcb.59.3.766 Crissman, 1976, Rapid staining methods for analysis of deoxyribonucleic acid and protein in mammalian cells, J. Histochem. Cytochem., 24, 64, 10.1177/24.1.56392 Frankfurt, 1980, Flow cytometric analysis of double-stranded RNA content distributions, J. Histochem. Cytochem., 28, 663, 10.1177/28.7.6156201 Hudson, 1969, The use of an ethidium analogue in the dye buoyant density procedure for the isolation of closed circular DNA: the variation of the superhelix density of mitochondrial DNA, 62, 813 Krishan, 1976, Flow microflurometric patterns of human bone marrow and tumor cells in response to cancer chemotheraphy, Cancer Res., 36, 3813 Matsuo, 1982, Ploidy of human embyonic fibroblasts during in vitro aging, J. Gerontol., 37, 33, 10.1093/geronj/37.1.33 Kihara, 1981, Detection of mycoplasmal contaminants in sera, J. Biol. Standard., 9, 243, 10.1016/S0092-1157(81)80049-2 Yamamoto, 1988, Changes in negative surface charge of human diploid fibroblasts, TIG-1, during in vitro aging, Mech. Ageing Dev., 42, 183, 10.1016/0047-6374(88)90073-5 Scott, 1956, Studies in histochemistry:, 4, 1 Mejbaum, 1939, Estimation of small amounts of pentose especially in derivatives of adenylic acid, Hoppe-Seyler's Z. Phys. Chem., 258, 117, 10.1515/bchm2.1939.258.2-3.117 Burton, 1956, A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid, Biochem. J., 62, 315, 10.1042/bj0620315 Lowry, 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193, 265, 10.1016/S0021-9258(19)52451-6 Cristofalo, 1973, Cellular senecence and DNA synthesis: thymidine incorporation as a measure of population age in human diploid cells, Exp. Cell Res., 76, 419, 10.1016/0014-4827(73)90394-7 Grove, 1977, Characterization of the cell cycle of cultured human diploid cells: Effects of aging and hydrocortisone, J. Cell. Physiol., 90, 415, 10.1002/jcp.1040900305 Johnson, 1977, The relative amounts of the cytoplasmic RNA species in normal, transformed and senescent cultured cell lines, J. Cell. Physiol., 90, 465, 10.1002/jcp.1040900310 Wolf, 1980, Regulation of ribosomal ribonucleic acid levels in growing, 3H-arrested, and crisis-phase WI-38 human diploid fibroblasts, Biochemistry, 19, 3484, 10.1021/bi00556a012 Adam, 1987, Level of ribosomal RNA required for stimulation from quiescence increases during cellular aging in vitro of mammalian fibroblasts, Exp. Cell Res., 169, 345, 10.1016/0014-4827(87)90195-9 Macieira-Coelho, 1973, Aging in vitro, 2, 13 Chen, 1980, RNA and protein synthesis in cultured human fibroblasts derived from donors of various ages, Mech. Ageing Dev., 13, 285, 10.1016/0047-6374(80)90041-X Michl, 1967, RNA turnover and the growth potential of human cells in culture, Exp. Cell Res., 47, 616, 10.1016/0014-4827(67)90019-5 Killander, 1965, Quantitative cytochemical studies on interphase growth, 38, 272 Mitsui, 1976, Relationship between cell replication and volume in senescent human diploid fibroblasts, Mech. Ageing Dev., 5, 45, 10.1016/0047-6374(76)90007-5 Darzynkiewicz, 1980, Subcompartments of the G1 phase of cell cycle detected by flow cytometry, 77, 6696 Stancel, 1981, Most of the G1 period in hamster cells is eliminated by lengthening the S period, 78, 6295 Fujikawa-Yamamoto, 1982, RNA dependence in the cell cycle of V79 cells, J. Cell. Physiol., 112, 60, 10.1002/jcp.1041120110 Darzynkiewicz, 1982, Cell heterogenecity during the cell cycle, J. Cell. Physiol., 113, 465, 10.1002/jcp.1041130316 Olashaw, 1983, Thymidine triphosphate synthesis in senescent WI38 cells, 149, 547 Gorman, 1984, Evidence that senescent WI-38 cells are brocked in late G1, In Vitro, 20, 281 Ohno, 1986, Distribution of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining, Exp. Cell Res., 163, 309, 10.1016/0014-4827(86)90062-5 Rittling, 1986, Expression of cell cycle-dependent genes in young and senescent WI-38 fibroblasts, 83, 3316 Seuwen, 1983, Only one of the two signals required for initiation of the cell cycle is associated with cellular accumulation of ribosomal RNA, Biochem. Biophys. Res. Commun., 117, 223, 10.1016/0006-291X(83)91564-4 Seuwen, 1984, Cellular content of ribosomal RNA in relation to the progression and competence signals governing proliferation of 3T3 and SV40-3T3 cells, Exp. Cell Res., 154, 10, 10.1016/0014-4827(84)90664-5 Darzynkiewicz, 1975, Conformation of RNA in situ as studied by acridine orange staining and automated cytofluorometry, Exp. Cell Res., 95, 143, 10.1016/0014-4827(75)90619-9 Lumpkin, 1986, Existence of high abundance antiproliferative mRNA's in senescent human diploid fibroblasts, Science, 232, 393, 10.1126/science.2421407 Jongkind, 1982, Isolation of autofluorescent “aged” human fibroblasts by flow sorting, Exp. Cell Res., 138, 409, 10.1016/0014-4827(82)90189-6 Bowman, 1975, Characteristics of proliferative cells from young, old and transformed WI-38 cultures, 53, 107