The Impact of Different DNA Extraction Kits and Laboratories upon the Assessment of Human Gut Microbiota Composition by 16S rRNA Gene Sequencing

PLoS ONE - Tập 9 Số 2 - Trang e88982
Nicholas A. Kennedy1, Alan W. Walker2, Susan H. Berry3, Sylvia H. Duncan4, Freda M. Farquarson4, Petra Louis4, John Thomson5, Jack Satsangi1, Harry J. Flint4, Julian Parkhill2, Charlie W. Lees1, Georgina L. Hold3
1Gastrointestinal Unit, Centre for Genomic and Experimental Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom.
2Pathogen Genomics Group, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, United Kingdom
3Gastrointestinal Research Group, Division of Applied Medicine, Aberdeen University, Aberdeen, United Kingdom
4Rowett Institute of Nutrition and Health, Aberdeen University, Aberdeen, United Kingdom.
5Department of Digestive Disorders, Aberdeen Royal Infirmary, Foresterhill, Aberdeen, United Kingdom.

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