Disease surveillance in recombining pathogens: Multilocus genotypes identify sources of human Coccidioides infections

Proceedings of the National Academy of Sciences of the United States of America - Tập 99 Số 13 - Trang 9067-9071 - 2002
Matthew C. Fisher1, Bruce Rannala1, Vishnu Chaturvedi1, John W. Taylor1
1The Institute of Zoology, Regent's Park, London NW1 4RY, United Kingdom; Department of Medical Genetics, University of Alberta, Edmonton, AB, Canada T6G 2H7; Mycology Laboratory, Wadsworth Center, 120 New Scotland Avenue, Albany, NY 12201-2002; and Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720

Tóm tắt

Molecular surveillance of pathogenic microbes works by genotyping isolates with DNA fingerprinting techniques and then using these genotypes to assign individuals to populations. Clonality is assumed in many fingerprinting studies, although this assumption has been shown to be false for many organisms. To accommodate recombining organisms into surveillance programs, methods using population allele frequencies in combination with individual multilocus genotypes are necessary. Here, we develop a statistical method appropriate for haploid recombining microbes that allows individuals to be assigned to populations. We illustrate the usefulness of this technique by inferring the source populations for Coccidioides isolates recovered from patients treated outside the endemic area of Coccidioides sp., the etiological agents of human coccidioidomycosis, but with a travel history including visits to one or more endemic areas.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 99 Số 13

9067-9071

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