Complete genome sequence of pepper yellow mosaic virus, a potyvirus, occurring in Brazil

Archives of Virology - Tập 157 - Trang 1397-1401 - 2012
N. Lucinda1, W. B. da Rocha2, A. K. Inoue-Nagata3, T. Nagata4
1Pós-graduação em Fitopatologia, Universidade de Brasília, Brasília, Brazil
2Ciências Biológicas, Universidade Católica de Brasília, Brasília, Brazil
3Embrapa Hortaliças, Brasília, Brazil
4Departamento de Biologia Celular, Universidade de Brasília, Brasília, Brazil

Tóm tắt

The complete genomic sequence of pepper yellow mosaic virus (PepYMV), a member of the genus Potyvirus, was determined. The sequence was 9745 nucleotides long, excluding the 3′ poly(A) tail. The genome contained a large open reading frame encoding a polyprotein of 3085 amino acids, which contained the typically conserved motifs found in members of the genus Potyvirus and an additional P3-PIPO (pretty interesting potyvirus ORF). In a pairwise comparison with other potyvirus sequences, the full genome of PepYMV shared a maximum of 63.84 % nucleotide sequence identity with pepper mottle virus (PepMoV), followed by verbena virus Y (VVY, 62.11 %), potato virus Y (PVY, 62.07 %) and Peru tomato mosaic virus (PTV, 62.00 %). Based upon a phylogenetic analysis, PepYMV was most closely related to PepMoV and PTV, within the PVY subgroup cluster, like most potyviruses isolated in solanaceous hosts in South America.

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