Spatial patterning of endothelial cells and vascular network formation using ultrasound standing wave fields

Journal of the Acoustical Society of America - Tập 134 Số 2 - Trang 1483-1490 - 2013
Kelley A. Garvin1, Diane Dalecki1, Mohammed Yousefhussien2, María Helguera2, Denise C. Hocking3
1University of Rochester Department of Biomedical Engineering, , Rochester, New York 14627
2Chester F. Carlson Center for Imaging Science, Rochester Institute of Technology , Rochester, New York 14623
3University of Rochester Department of Pharmacology and Physiology, , Rochester, New York 14642

Tóm tắt

The spatial organization of cells is essential for proper tissue assembly and organ function. Thus, successful engineering of complex tissues and organs requires methods to control cell organization in three dimensions. In particular, technologies that facilitate endothelial cell alignment and vascular network formation in three-dimensional tissue constructs would provide a means to supply essential oxygen and nutrients to newly forming tissue. Acoustic radiation forces associated with ultrasound standing wave fields can rapidly and non-invasively organize cells into distinct multicellular planar bands within three-dimensional collagen gels. Results presented herein demonstrate that the spatial pattern of endothelial cells within three-dimensional collagen gels can be controlled by design of acoustic parameters of the sound field. Different ultrasound standing wave field exposure parameters were used to organize endothelial cells into either loosely aggregated or densely packed planar bands. The rate of vessel formation and the morphology of the resulting endothelial cell networks were affected by the initial density of the ultrasound-induced planar bands of cells. Ultrasound standing wave fields provide a rapid, non-invasive approach to pattern cells in three-dimensions and direct vascular network formation and morphology within engineered tissue constructs.

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Tài liệu tham khảo

2006, Tissue-engineered autologous bladders for patients needing cystoplasty, Lancet, 367, 1241, 10.1016/S0140-6736(06)68438-9

1999, A novel inert collagen matrix for hypospadias repair, J. Urol., 162, 1148, 10.1016/S0022-5347(01)68105-9

2010, Directed 3D cell alignment and elongation in microengineered hydrogels, Biomaterials, 31, 6941, 10.1016/j.biomaterials.2010.05.056

2012, Engineered whole organs and complex tissues, Lancet, 379, 943, 10.1016/S0140-6736(12)60073-7

2010, Tissue-engineered tracheal transplantation, Transplantation, 89, 485, 10.1097/TP.0b013e3181cd4ad3

2006, Gap junctional intercellular communication and cytoskeletal organization in chondrocytes in suspension in an ultrasound trap, Mol. Membr Biol., 23, 195, 10.1080/09687860600555906

2005, Physical environment of 2-D animal cell aggregates formed in a short path length ultrasound standing wave trap, Ultrasound Med. Biol., 31, 423, 10.1016/j.ultrasmedbio.2004.12.007

2008, Engineering cartilage tissue, Adv. Drug Delivery Rev., 60, 243, 10.1016/j.addr.2007.08.027

1994, Ultrasonic manipulation of particles and cells. Ultrasonic separation of cells, Bioseparation, 4, 73

1974, The production of blood cell stasis and endothelial damage in the blood vessels of chick embryos treated with ultrasound in a stationary wave field, Ultrasound Med. Biol., 1, 133, 10.1016/0301-5629(74)90003-9

2003, Urethral stricture repair with an off-the-shelf collagen matrix, J. Urol., 169, 170, 10.1016/S0022-5347(05)64060-8

2011, Vascularization of three-dimensional collagen hydrogels using ultrasound standing wave fields, Ultrasound Med. Biol., 37, 1853, 10.1016/j.ultrasmedbio.2011.07.008

2010, Controlling the spatial organization of cells and extracellular matrix proteins in engineered tissues using ultrasound standing wave fields, Ultrasound Med. Biol., 36, 1919, 10.1016/j.ultrasmedbio.2010.08.007

2005, A new immobilization method to arrange particles in a gel matrix by ultrasound standing waves, Ultrasound Med. Biol., 31, 261, 10.1016/j.ultrasmedbio.2004.10.010

Rozenberg, 1971, Radiation forces acting on a particle in a sound field, High Intensity Ultrasonic Fields, 109, 10.1007/978-1-4757-5408-7

2008, Digital Image Processing, 58

1962, On the forces acting on a small particle in an acoustical filed in an ideal fluid, Sov. Phys. Dokl., 6, 773

Bjorno, 1974, The effects of acoustic forces on small particles in suspension, Finite Amplitude Wave Effects in Fluids: Proceedings of the 1973 Symposium, 252

2005, Diffusion limits of an in vitro thick prevascularized tissue, Tissue Eng., 11, 257, 10.1089/ten.2005.11.257

2009, Artificial corneas: A regenerative medicine approach, Eye, 23, 1985, 10.1038/eye.2008.409

1979, Statistical and structural approaches to texture, Proc. IEEE, 67, 786, 10.1109/PROC.1979.11328

2009, Application of magnetic force-based cell patterning for controlling cell-cell interactions in angiogenesis, Biotechnol. Bioeng., 102, 882, 10.1002/bit.22104

2012, Progress in the tissue engineering and stem cell industry ‘are we there yet?’, Tissue Eng. Part B Rev., 18, 155, 10.1089/ten.teb.2011.0553

2009, Progress in tissue engineering, Sci. Am., 300, 64, 10.1038/scientificamerican0509-64

1999, Tensional forces in fibrillar extracellular matrices control directional capillary sprouting, J. Cell. Sci., 112, 3249

2009, Guidance of vascular development: Lessons from the nervous system, Circ. Res., 104, 428, 10.1161/CIRCRESAHA.108.188144

2007, Tissue-engineered blood vessel for adult arterial revascularization, N. Engl. J. Med., 357, 1451, 10.1056/NEJMc071536

2008, Clinical transplantation of a tissue-engineered airway, Lancet, 372, 2023, 10.1016/S0140-6736(08)61598-6

2009, Effectiveness of haemodialysis access with an autologous tissue-engineered vascular graft: A multicenter cohort study, Lancet, 373, 1440, 10.1016/S0140-6736(09)60248-8

2012, Transplantation of an allogeneic vein bioengineered with autologous stem cells: A proof-of-concept study, Lancet, 380, 230, 10.1016/S0140-6736(12)60633-3

2011, Tissue engineering: Current strategies and future directions, Chonnam Med. J., 47, 1, 10.4068/cmj.2011.47.1.1

2009, Segmentation of retinal vasculature in color images, Innovative Technologies in Intelligent Systems and Industrial Applications, 398

2008, Forced alignment of mesenchymal stem cells undergoing cardiomyogenic differentiation affects functional integration with cardiomyocyte cultures, Circ. Res., 103, 167, 10.1161/CIRCRESAHA.108.176131

2008, Skin tissue engineering for tissue repair and regeneration, Tissue Eng. Part B Rev., 14, 105, 10.1089/teb.2007.0318

2010, Geometrically controlled endothelial tubulogenesis in micropatterned gels, Tissue Eng. Part A, 16, 2255, 10.1089/ten.tea.2009.0584

2011, Tissue-engineered autologous urethras for patients who need reconstruction: An observational study, Lancet, 377, 1175, 10.1016/S0140-6736(10)62354-9

1998, Fabrication of a polymer composite with periodic structure by the use of ultrasonic waves, J. Appl. Phys., 83, 3490, 10.1063/1.366561

2001, Transplantation of a tissue-engineered pulmonary artery, N. Engl. J. Med., 344, 532, 10.1056/NEJM200102153440717

2010, Micropatterning as a tool to decipher cell morphogenesis and functions, J. Cell Sci., 123, 4201, 10.1242/jcs.075150

1992, Particle column formation in a stationary ultrasonic field, J. Acoust. Soc. Am., 91, 79, 10.1121/1.402622

2012, In vitro microvessels for the study of angiogenesis and thrombosis, Proc. Natl. Acad. Sci. U.S.A., 109, 9342, 10.1073/pnas.1201240109