Probiotic effect of lactic acid bacteria in the feed on growth and survival of fry of Atlantic cod (Gadus morhua)
Tóm tắt
A growing concern for the high consumption of antibiotics in aquaculture has initiated a search for alternative methods of disease control. Improved resistance against infectious diseases can be achieved by the use of probiotics. Probiotics are live microorganisms supplemented in food or feed which give beneficial effects on the intestinal microbial balance. In the present study a dry feed containing lactic acid bacteria (Carnobacterium divergens) isolated from Atlantic cod (Gadus morhua)intestines was given to cod fry. After three weeks of feeding the fry was exposed to a virulent strain of Vibrio anguillarum. The death rate was recorded during further three weeks of feeding with lactic acid bacteria supplemented feed. A certain improvement of disease resistance was obtained, and at the end of the experiment lactic acid bacteria dominated the intestinal flora in surviving fish given feed supplemented with lactic acid bacteria. No obvious growth inhibition of V. anguillarum was observed in an in vitro mixed culture of this bacterium and the C. divergens isolated from cod intestines.
Tài liệu tham khảo
Bhattacharyya, G. K. & R. A. Johnson, 1977. Comparison of two binomial proportions. In Bradly, R. A., J. S. Hunter, D. G. Kendall & G. S. Watson (eds), Statistical Consepts and Methods. Wiley & Sons, New York: 308–333.
Bøgwald, J., R. A. Dalmo, R. M. Leifson, E. Stenberg & A. Gildberg, 1996. The stimulatory effect of a muscle protein hydrolysate from Atlantic cod, Gadus morhuaL., head kidney leucocytes. Fish Shellfish Immunol. 6: 3–16.
Bøgwald, J., K. Stensvåg, J. Hoffmann, K. O. Holm & T. Jørgensen, 1992. Vaccination of Atlantic salmon, Salmo salarL., with particulate lipo-poly-saccharide antigens from Vibrio salmonicida and Vibrio anguillarum.Fish Shellfish Immunol. 2: 251–261.
Espe, M. & E. Lied, 1994. Do Atlantic salmon (Salmo salar)utilize mixtures of free amino acids to the same extent as intact protein sources for muscle protein synthesis? Comp. Biochem. Physiol. 107A: 249–254.
Fagbenro, O. & K. Jauncey, 1994. Growth and protein utilization by juvenile catfish (Clarias gariepinus)fed moist diets containing autolysed protein from stored lactic acid-fermented fish-silage. Bioresour. Technol. 48: 43–48.
Ford, J. E., K. D. Perry & C. A. E. Briggs, 1958. Nutrition of lactic acid bacteria isolated from rumen. J. Gen. Microbiol. 18: 273–284.
Fuller, R., 1989. Probiotics in man and animal. J. appl. Bacteriol. 66: 365–378.
Gatesoupe, F.-J., 1994. Lactic acid bacteria increase the resistance of turbot larvae, Scophthalamus maximus, against pathogenic vibrio. Aquat. Living Resour. 7: 277–282.
Gildberg, A., J. Bøgwald, A. Johansen & E. Stenberg, 1996. Isolation of acid peptide fractions from a fish protein hydrolysate with strong stimulatory effect on Atlantic salmon (Salmo salar)head kidney leucocytes. Comp. Biochem. Physiol. 114B: 97–101.
Gildberg, A., A. Johansen & J. Bøgwald, 1995. Growth and survival of Atlantic salmon (Salmo salar)fry given diets supplemented with fish protein hydrolysate and lactic acid bacteria during a challenge trial with Aeromonas salmonicida. Aquacult. 138: 2334.
Hamnes, W. P., N. Weiss & W. Holzapfel, 1992. The genera Lactobacillus and Carnobacterium.In Balows, A., H. G. Truper, M. Dworkin, W. Harder & K.-H. Schleifer (eds), The prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications. Vol. II. Springer-Verlag, New York: 1536–1594.
Holck, A., L. Axelsson & U. Schillinger, 1996. Divergicin 750, a novel bacteriocin produced by Carnobacterium divergens750. FEMS Microbiol. Letters 136: 163–168.
Leifson, R. M., 1996. Oralvaksinering av Atlantisk laks (Salmo salarL.) ved bruk av liposom/alginat innpakket vaksine. M. Sci. Thesis. The Norwegian College of Fishery Science. 103 pp (in Norwegian).
Muroga, K., M. Higashi & H. Keitoku, 1987. The isolation of intestinal micro-flora of farmed red seabream (Acanthopagrus schlegeli) at larval and juvenile stages. Aquaculture 65: 79–88.
Newman, S. G. & R. Deupree, 1995. Biotechnology in aquaculture in aquaculture. Infofish Internat. 1/95: 40–46.
Nousiainen, J. & J. Setälä, 1993. Lactic acid bacteria as animal probiotics. In Salminen, S. & A. von Wright (eds), Lactic Acid Bacteria. Marcel Dekker, New York: 315–356.
Pilet, M.-F., X. Dousset, R. Barré, G. Novel, M. Desmazeaud & J.-C. Piard, 1995. Evidence for two bacteriocins produced by Carnobacterium piscicolaand Carnobacterium diveregensisolated from fish and active against Listeria monocytogenes.J. Food Protect. 58: 256–262.
Raa, J., 1996. The use of immunostimulatory substances in fish and shellfish farming. Rev. Fish. Sci. 4: 229–288.
Siwicki, A. K., D. P. Anderson & O. W. Dixon, 1990. In vitro immunostimulation of rainbow trout (Oncorhynchus mykiss) spleen cells with levamisole. Developmental Comp. Immunol. 14: 231–237.
Stavric, S. & T. Kornegay, 1995. Microbial probiotics for pigs and poultry. In Wallace, R. J. & A. Chesson (eds), Biotechnology in Animal Feeds and Animal Feeding, Weinheim, New York: 205–231.
Strøm, E., 1988. Melkesyrebakterier i fisketarm. Isolasjon, karakterisering og egenskaper. M. Sci. Thesis. The Norwegian College of Fishery Science, 88 pp (in Norwegian).
Strøm, E. & E. Ringø, 1993. Changes in the bacterial composition of early developing cod, Gadus morhua(L.) larvae following inoculation of Lactobacillus plantaruminto the water. In Walther, B. & H. J. Fyhn (eds), Physiological and Biochemical Aspects of Fish Larval Development. University of Bergen, Bergen, Norway: 226–228.