DNA double-strand breaks in mouse kidney cells with age

Biogerontology - Tập 2 - Trang 261-270 - 2001
Narendra P. Singh1, Charles E. Ogburn2, Norman S. Wolf2, Gerald van Belle3, George M. Martin4
1Department of Bioengineering, University of Washington, Seattle, USA
2Department of Pathology, University of Washington, Seattle, USA
3Departments of Bio-statistics and Environmental Health, University of Washington, Seattle, USA
4Department of Genetics and Department of Pathology, University of Washington, Seattle, USA

Tóm tắt

A Biojector device fitted with a CO2 cartridge was used to prepare single cellsuspensions from kidneys of 12-month-(middle-aged) and 24-month-old (old) C57Bl/6mice. Microgel electrophoresis of DNA fromthese cells revealed a modest but significant7.3% increase (P = 0.04) in DNA double-strand breaks in old mice. This increase is equivalent to the DNA damage induced by 0.1 Gray of X-rays (5 double-strand breaks) in kidney cells of 10-month-old mice, as determined by a standard calibration curve. Greater DNA damage with aging was also positively correlated with higher levels of pathology in the kidneys.

Tài liệu tham khảo

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