Comparative immunofluorescent studies on measles, canine distemper, and rinderpest viruses
Tóm tắt
The development of specific antigens of measles, canine distemper and rinderpest viruses in Vero cells was examined by fluorescent antibody (FA) techniques. Intracellular and extracellular virus antigens were demonstrated by FA staining of fixed cells and unfixed living cells, respectively. All the three viruses showed the same pattern of development of specific antigens in the same host cells. Both V and S antigens were considered to be synthesized exclusively in the cytoplasm. The antigenic correlation among the three viruses was also studied by cross FA staining of both fixed and unfixed samples. A significant correlation among them was more clearly demonstrated by FA technique than in virus neutralization, HI, or CF tests. The similarity in the structures of V antigens as well as S antigens among the three viruses is discussed.
Tài liệu tham khảo
Coffin, D. L., andC. Liu: Studies on canine distemper infection by means of fluorescein-labeled antibody. II. The pathology and diagnosis of the naturally occurring disease in dogs and the antigenic nature of the inclusion body. Virology3, 132–145 (1957).
Imagawa, D. T.: Relationship among measles, canine distemper and rinderpest viruses. Progr. med. Virol.10, 160–193 (1968).
Keloff, G., andP. K. Vogt: Localization of avian tumor virus group-specific antigen in cell and virus. Virology29, 377–384 (1966).
Liess, B.: Fluoreszenzserologische Untersuchungen an Zellkulturen nach Infektion mit Rinderpestvirus. Zbl. Bakt. I. Abt. Orig.190, 424–443 (1963).
Möller, G.: Demonstration of mouse isoantigens at the cellular level by the fluorescent antibody technique. J. exp. Med.114, 415–432 (1961).
Rapp, F.: Plaque differentiation and replication of virulent and attenuated strains of measles virus. J. Bact.88, 1448–1458 (1964).
Rapp, F., I. Gordon, andR. F. Baker: Observations of measles virus infection of cultured human cells. I. A study of development and spread of virus antigen by means of immunofluorescence. J. biophys. biochem. Cytol.7, 43–48 (1960).
Reda, I. M., R. Rott, andW. Schäfer: Fluorescent antibody studies with NDV-infected cell systems. Virology22, 422–425 (1964).
Roizman, B., andA. E. Schluederberg: Virus infection of cells in mitosis. II. Measles virus infection of mitotic Hep-2 cells. Proc. Soc. exp. Biol. (N.Y.)106, 320–323 (1961).
Shishido, A., K. Yamanouchi, M. Hikita, T. Sato, A. Fukuda, andF. Kobune: Development of a cell culture system susceptible to measles, canine distemper, and rinderpest viruses. Arch. ges. Virusforsch.22, 364–380 (1967).
Toyoshima, K., S. Hata, M. Takahashi, T. Miki, andY. Okuno: Virological studies of measles virus. III. Morphological changes and virus growth in FL cultures. Biken's J.3, 241–248 (1960).
Waterson, A. P., R. Rott, andG. Ruckle-Enders: The components of measles virus and their relation to rinderpest and distemper. Z. Naturforsch.18b, 377–384 (1963).
Yamanouchi, K., A.Fukuda, F.Kobune, M.Hayami, and A.Shishido: Serologic response in monkeys inoculated with rinderpest and measles viruses. Amer. J. vet. Res. (In Press.)