Length-dependent recognition of double-stranded ribonucleic acids by retinoic acid–inducible gene-I and melanoma differentiation–associated gene 5

Journal of Experimental Medicine - Tập 205 Số 7 - Trang 1601-1610 - 2008
Hiroki Kato1,2, Osamu Takeuchi1,2, Eriko Mikamo-Satoh3,4, Reiko Hirai5, Tomoji Kawai3, Kazufumi Matsushita1,2, Akane Hiiragi6, Terence S. Dermody7, Takashi Fujita5,6, Shizuo Akira1,2
11Laboratory of Host Defense, World Premiere International Immunology Frontier Research Center,
22Research Institute for Microbial Diseases,
33Institute for Scientific and Industrial Research, Osaka University, Suita, Osaka 565-0871, Japan
44Department of Pharmacy, Hyogo University of Health Sciences, Cyuo-ku, Kobe, Hyogo 650-8530, Japan
55Laboratory of Molecular Genetics, Institute for Virus Research, and
66Laboratory of Molecular Cell Biology, Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan
77Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232

Tóm tắt

The ribonucleic acid (RNA) helicases retinoic acid-inducible gene-I (RIG-I) and melanoma differentiation–associated gene 5 (MDA5) recognize distinct viral and synthetic RNAs, leading to the production of interferons. Although 5′-triphosphate single-stranded RNA is a RIG-I ligand, the role of RIG-I and MDA5 in double-stranded (ds) RNA recognition remains to be characterized. In this study, we show that the length of dsRNA is important for differential recognition by RIG-I and MDA5. The MDA5 ligand, polyinosinic-polycytidylic acid, was converted to a RIG-I ligand after shortening of the dsRNA length. In addition, viral dsRNAs differentially activated RIG-I and MDA5, depending on their length. Vesicular stomatitis virus infection generated dsRNA, which is responsible for RIG-I–mediated recognition. Collectively, RIG-I detects dsRNAs without a 5′-triphosphate end, and RIG-I and MDA5 selectively recognize short and long dsRNAs, respectively.

Từ khóa


Tài liệu tham khảo

2006, Cell., 124, 783, 10.1016/j.cell.2006.02.015

2007, Nat. Rev. Immunol., 7, 753, 10.1038/nri2174

2007, Nature., 449, 819, 10.1038/nature06246

2007, Biochimie., 89, 754, 10.1016/j.biochi.2007.01.013

2001, Nature., 413, 732, 10.1038/35099560

2004, Science., 303, 1529, 10.1126/science.1093616

2004, Science., 303, 1526, 10.1126/science.1093620

2000, Nature., 408, 740, 10.1038/35047123

1998, Immunity., 9, 143, 10.1016/S1074-7613(00)80596-8

2003, Science., 301, 640, 10.1126/science.1087262

2005, Annu. Rev. Immunol., 23, 275, 10.1146/annurev.immunol.23.021704.115633

2004, Nat. Immunol., 5, 730, 10.1038/ni1087

2004, Proc. Natl. Acad. Sci. USA., 101, 17264, 10.1073/pnas.0407639101

2005, J. Immunol., 175, 5260, 10.4049/jimmunol.175.8.5260

2005, Nat. Immunol., 6, 981, 10.1038/ni1243

2006, J. Exp. Med., 203, 1795, 10.1084/jem.20060792

2005, Cell., 122, 669, 10.1016/j.cell.2005.08.012

2006, Immunity., 24, 633, 10.1016/j.immuni.2006.04.004

2005, Nature., 437, 1167, 10.1038/nature04193

2005, Mol. Cell., 19, 727, 10.1016/j.molcel.2005.08.014

2000, Immunity., 13, 539, 10.1016/S1074-7613(00)00053-4

2005, Nature., 434, 772, 10.1038/nature03464

2005, J. Immunol., 175, 2851, 10.4049/jimmunol.175.5.2851

2007, Proc. Natl. Acad. Sci. USA., 104, 582, 10.1073/pnas.0606699104

2007, J. Immunol., 178, 6444, 10.4049/jimmunol.178.10.6444

2006, Nature., 441, 101, 10.1038/nature04734

2008, J. Virol., 82, 335, 10.1128/JVI.01080-07

2006, Proc. Natl. Acad. Sci. USA., 103, 8459, 10.1073/pnas.0603082103

2006, Science., 314, 994, 10.1126/science.1132505

2006, Science., 314, 997, 10.1126/science.1132998

2006, Nat. Biotechnol., 24, 559, 10.1038/nbt1205

1955, Science., 122, 907, 10.1126/science.122.3176.907

2006, J. Virol., 80, 5059, 10.1128/JVI.80.10.5059-5064.2006

2004, Nat. Biotechnol., 22, 321, 10.1038/nbt940

2007, Nature., 448, 816, 10.1038/nature06042

1995, Virology., 206, 760, 10.1016/S0042-6822(95)80005-0

2005, Immunity., 23, 19, 10.1016/j.immuni.2005.04.010

2002, J. Virol., 76, 1632, 10.1128/JVI.76.4.1632-1641.2002

2005, J. Struct. Biol., 151, 106, 10.1016/j.jsb.2005.05.002

Thông tin
Thông tin xuất bản

Journal of Experimental Medicine

Tập 205 Số 7

1601-1610

Thông tin tác giả