Enzymatic activities of Sir2 and chromatin silencing

Karpen, 1994, Position-effect variegation and the new biology of heterochromatin, Curr Opin Genet Dev, 4, 281, 10.1016/S0959-437X(05)80055-3 Kellum, 1995, Heterochromatin protein 1 is required for correct chromosome segregation in Drosophila embryos, J Cell Sci, 108, 1419, 10.1242/jcs.108.4.1419 Allshire, 1994, Position effect variegation at fission yeast centromeres, Cell, 76, 157, 10.1016/0092-8674(94)90180-5 Ekwall, 1999, Fission yeast mutants that alleviate transcriptional silencing in centromeric flanking repeats and disrupt chromosome segregation, Genetics, 153, 1153, 10.1093/genetics/153.3.1153 Herskowitz, 1992, Mating-type determination and mating-type interconversion in Saccharomyces cerevisiae, 583 Klar, 1981, Regulation of transcription in expressed and unexpressed mating type cassettes of yeast, Nature, 289, 239, 10.1038/289239a0 Grewal, 2000, Transcriptional silencing in fission yeast, J Cell Physiol, 184, 311, 10.1002/1097-4652(200009)184:3<311::AID-JCP4>3.0.CO;2-D Gottschling, 1990, Position effect at S. Cerevisiae telomeres: reversible repression of Pol II transcription, Cell, 63, 751, 10.1016/0092-8674(90)90141-Z Gartenberg, 2000, The Sir proteins of Saccharomyces cerevisiae: mediators of transcriptional silencing and much more, Curr Opin Microbiol, 3, 132, 10.1016/S1369-5274(00)00064-3 Frye, 1999, Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity, Biochem Biophys Res Commun, 260, 273, 10.1006/bbrc.1999.0897 Tanny, 1999, An enzymatic activity in the yeast Sir2 protein that is essential for gene silencing, Cell, 99, 735, 10.1016/S0092-8674(00)81671-2 Imai, 2000, Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase, Nature, 403, 795, 10.1038/35001622 Landry, 2000, The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases, Proc Natl Acad Sci USA, 97, 5807, 10.1073/pnas.110148297 Smith, 2000, A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family, Proc Natl Acad Sci USA, 97, 6658, 10.1073/pnas.97.12.6658 Klar, 1979, MAR1 — a regulator of HMa and HMα loci in Saccharomyces cerevisiae, Genetics, 93, 37, 10.1093/genetics/93.1.37 Rine, 1987, Four genes responsible for a position effect on expression from HML and HMR in Saccharomyces cerevisiae, Genetics, 116, 9, 10.1093/genetics/116.1.9 Aparicio, 1991, Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiae, Cell, 66, 1279, 10.1016/0092-8674(91)90049-5 Bryk, 1997, Transcriptional silencing of Ty1 elements in the RDN1 locus of yeast, Genes Dev, 11, 255, 10.1101/gad.11.2.255 Smith, 1997, An unusual form of transcriptional silencing in yeast ribosomal DNA, Genes Dev, 11, 241, 10.1101/gad.11.2.241 Moazed, 1996, A deubiquitinating enzyme interacts with SIR4 and regulates silencing in S. cerevisiae, Cell, 86, 667, 10.1016/S0092-8674(00)80139-7 Moazed, 1997, Silent information regulator protein complexes in Saccharomyces cerevisiae: a SIR2/SIR4 complex and evidence for a regulatory domain in SIR4 that inhibits its interaction with SIR3, Proc Natl Acad Sci USA, 94, 2186, 10.1073/pnas.94.6.2186 Strahl-Bolsinger, 1997, SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast, Genes Dev, 11, 83, 10.1101/gad.11.1.83 Straight, 1999, Net1, a Sir2-associated nucleolar protein required for rDNA silencing and nucleolar integrity, Cell, 97, 245, 10.1016/S0092-8674(00)80734-5 Shou, 1999, The termination of telophase is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from the nucleolar RENT complex, Cell, 97, 233, 10.1016/S0092-8674(00)80733-3 Cuperus, 2000, Locus specificity determinants in the multifunctional yeast silencing protein Sir2, EMBO J, 19, 2641, 10.1093/emboj/19.11.2641 Sinclair, 1997, Extrachromosomal rDNA circles – a cause of aging in yeast, Cell, 91, 1033, 10.1016/S0092-8674(00)80493-6 Kaeberlein, 1999, The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms, Genes Dev, 13, 2570, 10.1101/gad.13.19.2570 Kayne, 1988, Extremely conserved histone H4 N terminus is dispensable for growth but essential for repressing the silent mating loci in yeast, Cell, 55, 27, 10.1016/0092-8674(88)90006-2 Johnson, 1990, Genetic evidence for an interaction between SIR3 and histone H4 in the repression of the silent mating loci in Saccharomyces cerevisiae, Proc Natl Acad Sci USA, 87, 6286, 10.1073/pnas.87.16.6286 Thompson, 1994, Histone H3 amino terminus is required for telomeric and silent mating locus repression in yeast, Nature, 369, 245, 10.1038/369245a0 Braunstein, 1996, Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern, Mol Cell Biol, 16, 4349, 10.1128/MCB.16.8.4349 Hecht, 1995, Histone H3 and H4 N-termini interact with SIR3 and SIR4 proteins: a molecular model for the formation of heterochromatin in yeast, Cell, 80, 583, 10.1016/0092-8674(95)90512-X Brachmann, 1995, The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability, Genes Dev, 9, 2888, 10.1101/gad.9.23.2888 Frye, 2000, Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins, Biochem Biophys Res Commun, 273, 793, 10.1006/bbrc.2000.3000 Freeman-Cook, 1999, The Schizosaccharomyces pombe hst4(+) gene is a SIR2 homologue with silencing and centromeric functions, Mol Biol Cell, 10, 3171, 10.1091/mbc.10.10.3171 Xie, 1999, Sum1 and Hst1 repress middle sporulation-specific gene expression during mitosis in Saccharomyces cerevisiae, EMBO J, 18, 6448, 10.1093/emboj/18.22.6448 Tsang, 1998, CobB, a new member of the SIR2 family of eucaryotic regulatory proteins, is required to compensate for the lack of nicotinate mononucleotide: 5,6-dimethylbenzimidazole phosphoribosyltransferase activity in cobT mutants during cobalamin biosynthesis in Salmonella typhimurium LT2, J Biol Chem, 273, 31788, 10.1074/jbc.273.48.31788 Braunstein, 1993, Transcriptional silencing in yeast is associated with reduced nucleosome acetylation, Genes Dev, 7, 592, 10.1101/gad.7.4.592 Fresht, 1999 Landry, 2000, Role of NAD+ in the deacetylation activity of SIR2-like proteins, Biochem Biophys Res Commun, 278, 685, 10.1006/bbrc.2000.3854 Tanny JC, Moazed D: Coupling of histone deacetylation to NAD breakdown by the yeast silencing prtoein Sir2: evidence for acetyl transfer from substrate to an NAD breakdown product. Proc Natl Acad Sci USA 2001, 98: 415-420. (Published online December 26 2000.) This paper shows that the yeast Sir2 protein couples deacetylation to cleavage of a high-energy glycosidic bond in NAD with a 1:1 stoichiometry. Moreover, it is shown that Sir2 transfers acetyl groups from substrate to an NAD cleavage product to produce a novel compound, O-acetyl-ADP-ribose. Mutations in Sir2 are shown to affect its activities similarly and loss of activity in vitro is shown to correlate with loss of silencing in vivo. Tanner, 2000, Silent information regulator 2 family of NAD-dependent histone/protein deacetylases generates a unique product, 1-O-acetyl-ADP-ribose, Proc Natl Acad Sci USA, 97, 14178, 10.1073/pnas.250422697 Rowen, 1951, The phosphorolysis of nicotinamide riboside, J Biol Chem, 193, 497, 10.1016/S0021-9258(18)50905-4 Grunstein, 1997, Histone acetylation in chromatin structure and transcription, Nature, 389, 349, 10.1038/38664 Kuo, 1998, Roles of histone acetyltransferases and deacetylases in gene regulation, BioEssays, 20, 615, 10.1002/(SICI)1521-1878(199808)20:8<615::AID-BIES4>3.0.CO;2-H De Rubertis, 1996, The histone deacetylase RPD3 counteracts genomic silencing in Drosophila and yeast, Nature, 384, 589, 10.1038/384589a0 Rundlett, 1996, HDA1 and RPD3 are members of distinct yeast histone deacetylase complexes that regulate silencing and transcription, Proc Natl Acad Sci USA, 93, 14503, 10.1073/pnas.93.25.14503 Sun, 1999, A general requirement for the Sin3-Rpd3 histone deacetylase complex in regulating silencing in Saccharomyces cerevisiae, Genetics, 152, 921, 10.1093/genetics/152.3.921 Kelly, 2000, Type B histone acetyltransferase Hat1p participates in telomeric silencing, Mol Cell Biol, 20, 7051, 10.1128/MCB.20.19.7051-7058.2000 Reifsnyder, 1996, Yeast SAS silencing genes and hman genes associated with AML and HIV-1 Tat interactions are homolgous with acetyltransferases, Nat Genet, 14, 42, 10.1038/ng0996-42 Xu, 1999, SAS4 and SAS5 locus-specific regulators of silencing in Saccharomyces cerevisiae, Genetics, 153, 25, 10.1093/genetics/153.1.25 Grunstein, 1997, Molecular model for telomeric heterochromatin in yeast, Curr Opin Cell Biol, 9, 383, 10.1016/S0955-0674(97)80011-7 Loo, 1995, Silencing and heritable domains of gene expression, Annu Rev Cell Dev Biol, 11, 519, 10.1146/annurev.cb.11.110195.002511