Simultaneous harvesting and cell disruption of microalgae using ozone bubbles: optimization and characterization study for biodiesel production

Springer Science and Business Media LLC - Tập 15 - Trang 1257-1268 - 2021
Wan N. A. Kadir1,2, Man K. Lam1,2, Yoshimitsu Uemura2,3, Jun W. Lim2,4, Peck L. Kiew5, Steven Lim6, Siti S. Rosli2,4, Chung Y. Wong2,4, Pau L. Show7, Keat T. Lee8
1Chemical Engineering Department, Universiti Teknologi PETRONAS, Seri Iskandar, Malaysia
2HICoE-Centre for Biofuel and Biochemical Research, Institute of Self-Sustainable Building, Universiti Teknologi PETRONAS, Seri Iskandar, Malaysia
3NPO Kuramae Bioenergy, Tokyo, Japan
4Fundamental and Applied Sciences Department, Universiti Teknologi PETRONAS, Seri Iskandar, Malaysia
5Faculty of Engineering, Technology & Built Environment, University College Sedaya International, Cheras Kuala Lumpur, Malaysia
6Department of Chemical Engineering, Lee Kong Chian Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, Sungai Long, Malaysia
7Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Semenyih, Malaysia
8School of Chemical Engineering, Universiti Sains Malaysia, Engineering Campus, Nibong Tebal, Malaysia

Tóm tắt

In the present study, ozone was introduced as an alternative approach to harvest and disrupt microalgae cells (Chlorella vulgaris) simultaneously for biodiesel production. At the optimum ozonation conditions (6.14 g·h−1 ozone concentration, 30 min ozonation time, 1 L·min−1 of ozone flowrate at medium pH of 10 and temperature of 30 °C), the sedimentation efficiency of microalgae cells increased significantly from 12.56% to 68.62%. It was observed that the microalgae cells aggregated to form flocs after pretreated with ozone due to the increment of surface charge from −20 to −6.59 mV. Besides, ozone had successfully disrupted the microalgae cells and resulted in efficient lipid extraction, which was 1.9 times higher than the control sample. The extracted microalgae lipid was mainly consisted of methyl palmitate (C16:0), methyl oleate (C18:1) and methyl linolenate (C18:3), making it suitable for biodiesel production. Finally, utilization of recycled culture media after ozonation pre-treatment showed robust growth of microalgae, in which the biomass yield was maintained in the range of 0.796 to 0.879 g ·h−1 for 5 cycles of cultivation.

Tài liệu tham khảo

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