Longitudinal Studies of Clonally Expanded CD8 T Cells Reveal a Repertoire Shrinkage Predicting Mortality and an Increased Number of Dysfunctional Cytomegalovirus-Specific T Cells in the Very Elderly

Journal of Immunology - Tập 176 Số 4 - Trang 2645-2653 - 2006
Sine Reker Hadrup1,2, Jan Strindhall2, Tania Køllgaard1, Tina Seremet1, Boo Johansson3, Graham Pawelec4, Per thor Straten1, Anders Wikby2
1Tumor Immunology Group, Institute of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark
2†Department of Natural Science and Biomedicine, School of Health Sciences, Jönköping University, Jönköping, Sweden;
3‡Institute of Gerontology, School of Health Sciences, Jönköping University and Department of Psychology, Göteborg University, Göteborg, Sweden; and
4§University of Tübingen Medical School, Center for Medical Research, Tübingen, Germany

Tóm tắt

Abstract The age-associated decrease in functionality of the human immune system is thought to have a negative impact on the capacity to provide protection against infection, in turn leading to increased incidence of mortality. In a previous longitudinal study of octogenarians, we identified an immune risk phenotype (IRP) in the very elderly defined by an inverted CD4/CD8 ratio, which was associated with increased mortality and persistent CMV infection. In this study, we analyzed the CD8 clonal composition of nonagenarians and middle-aged individuals. An increased number of CD8 T cell clones was observed in the nonagenarians, and was associated with CMV-seropositivity. Surprisingly, CMV-seropositive nonagenarians with the IRP had a significantly lower number of clones compared with non-IRP individuals. The decrease in clone numbers in IRP individuals was associated with shorter survival time. MHC/peptide multimer staining indicated that the frequency of CMV-specific T cells was higher in nonagenarians than in the middle-aged, but the ratio of functionally intact cells was significantly lower. The lowest ratio of functional CMV-specific T cells was found in an IRP individual. A thorough longitudinal analysis of the CMV-specific T cells in nonagenarians showed a stable pattern with respect to frequency, phenotype, and clonal composition. We hypothesize that the number of different CD8 T cell clonal expansions increases as the individual ages, possibly, as a compensatory mechanism to control latent infections, e.g., CMV, but eventually a point is reached where clonal exhaustion leads to shrinkage of the CD8 clonal repertoire, associated with decreased survival.

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Journal of Immunology

Tập 176 Số 4

2645-2653

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