CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs

Cell Stem Cell - Tập 18 - Trang 541-553 - 2016
Mohammad A. Mandegar1, Nathaniel Huebsch1,2, Ekaterina B. Frolov1, Edward Shin1, Annie Truong1, Michael P. Olvera1, Amanda H. Chan1, Yuichiro Miyaoka1, Kristin Holmes1, C. Ian Spencer1, Luke M. Judge1,2, David E. Gordon3,4,5, Tilde V. Eskildsen6,7, Jacqueline E. Villalta3,4,8,9, Max A. Horlbeck3,4,8,9, Luke A. Gilbert3,4,8,9, Nevan J. Krogan3,4,5, Søren P. Sheikh6,7, Jonathan S. Weissman3,4,8,9, Lei S. Qi10
1Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
2Department of Pediatrics, University of California San Francisco, San Francisco, CA 94158, USA
3Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94158, USA
4California Institute for Quantitative Biosciences, QB3, University of California, San Francisco, San Francisco, CA 94158, USA
5Gladstone Institute of Virology and Immunology, San Francisco, CA 94158, USA
6Department of Cardiovascular and Renal Research, University of Southern Denmark, 5000 Odense C, Denmark
7Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, 5000 Odense C, Denmark
8Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA
9Center for RNA Systems Biology, University of California, San Francisco, San Francisco, CA 94158, USA
10Department of Bioengineering, Stanford University, Stanford, CA 94305, USA

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