Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of Fusarium oxysporum f. sp. cubense race 4
Tóm tắt
Fusarium wilt (Panama disease), caused by the fungus Fusarium oxysporum f. sp. cubense race 4 (Foc race 4), is one of the most destructive diseases affecting banana (Musa). Early and accurate detection of Foc race 4 is essential to protect the banana industry. We developed a novel and highly specific loop-mediated isothermal amplification (LAMP) assay for the detection of Foc race 4 based on a SCAR marker sequence. The detection limit for this assay was 10 fg per 25 μl reaction in pure culture and DNA amplification was completed within 60 min. The assays detected 69 different isolates of Foc race 4 from geographically distinct counties in China, and no cross-reaction was observed with other fungal pathogens. When 26 infected and eight healthy looking but infested banana samples naturally from different fields were examined, the detection rate of LAMP was 100 %. The LAMP assay developed in this study was simple, fast, sensitive, and specific, and can be used in the field to detect Foc race 4 in infected banana plant tissue in resource-poor settings.
Tài liệu tham khảo
Bentley, S., Pegg, K., Moore, N., Davis, R., & Buddenhagen, I. (1998). Genetic variation among vegetative compatibility groups of Fusarium oxysporum f. sp. cubense analyzed by DNA fingerprinting. Phytopathology, 88, 1283–1293.
Borges, A. A., Borges-Pérez, A., & Fernández-Falcón, M. (2004). Induced resistance to Fusarial wilt of banana by menadione sodium bisulphite treatments. Crop Protection, 23, 1245–1247.
Dita, M., Waalwijk, C., Buddenhagen, I., Souza, J. M., & Kema, G. (2010). A molecular diagnostic for tropical race 4 of the banana fusarium wilt pathogen. Plant Pathology, 59, 348–357.
Kaneko, H., Kawana, T., Fukushima, E., & Suzutani, T. (2007). Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances. Journal of Biochemical and Biophysical Methods, 70, 499–501.
Karam, N., Guglielmino, C., Bertin, S., Gomulski, L., Bonomi, A., Baldacchino, F., et al. (2008). RAPD analysis in the parasitoid wasp Psyttalia concolor reveals Mediterranean population structure and provides SCAR markers. Biological Control, 47, 22–27.
Li, X., Nie, J., Ward, L. J., Nickerson, J., & De Boer, S. H. (2011). Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection and identification of Pectobacterium atrosepticum. Canadian Journal of Plant Pathology, 33, 447–457.
Lin, Y. H., Chang, J. Y., Liu, E. T., Chao, C. P., Huang, J. W., & Chang, P. F. L. (2009). Development of a molecular marker for specific detection of Fusarium oxysporum f. sp. cubense race 4. European Journal of Plant Pathology, 123, 353–365.
Martin, F. N., Abad, Z. G., Balci, Y., & Ivors, K. (2012). Identification and detection of Phytophthora: reviewing our progress, identifying our needs. Plant Disease, 96, 1080–1103.
Molina, A., Fabregar, E., Sinohin, V., Fourie, G., & Viljoen, A. (2008). Tropical Race 4 of Fusarium oxysporum f. sp. cubense causing new Panama wilt epidemics in Cavendish varieties in the Philippines. Phytopathology, 98, S108.
Moradi, A., Nasiri, J., Abdollahi, H., & Almasi, M. (2012). Development and evaluation of a loop-mediated isothermal amplification assay for detection of Erwinia amylovora based on chromosomal DNA. European Journal of Plant Pathology, 133, 609–620.
Mori, Y., & Notomi, T. (2009). Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. Journal of Infection and Chemotherapy, 15, 62–69.
Nagamine, K., Watanabe, K., Ohtsuka, K., Hase, T., & Notomi, T. (2001). Loop-mediated isothermal amplification reaction using a nondenatured template. Clinical Chemistry, 47, 1742–1743.
Nagamine, K., Hase, T., & Notomi, T. (2002). Accelerated reaction by loop-mediated isothermal amplification using loop primers. Molecular and Cellular Probes, 16, 223–229.
Nakao, R., Stromdahl, E. Y., Magona, J. W., Faburay, B., Namangala, B., Malele, I., et al. (2010). Development of Loop-Mediated Isothermal Amplification (LAMP) assays for rapid detection of Ehrlichia ruminantium. BMC Microbiology, 10, 296.
Nauta, M. J., & Hoekstra, R. F. (1994). Evolution of vegetative incompatibility in filamentous ascomycetes. I. Deterministic models. Evolution, 48, 979–995.
Niessen, L., & Vogel, R. F. (2010). Detection of Fusarium graminearum DNA using a loop-mediated isothermal amplification (LAMP) assay. International Journal of Food Microbiology, 140, 183–191.
Niu, J., Jian, H., Guo, Q., Chen, C., Wang, X., Liu, Q., et al. (2012). Evaluation of loop–mediated isothermal amplification (LAMP) assays based on 5S rDNA-IGS2 regions for detecting Meloidogyne enterolobii. Plant Pathology, 61, 809–819.
Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., et al. (2000). Loop-mediated isothermal amplification of DNA. Nucleic Acids Research, 28, e63.
Ploetz, R. C. (2006). Fusarium wilt of banana is caused by several pathogens referred to as Fusarium oxysporum f. sp. cubense. Phytopathology, 96, 653–656.
Qi, Y. X., Xie, Y. X., Zhang, X., Pu, J. J., & Zhang, H. Q. (2006). The identification of pathogen causing banana Fusarium wilt in Hainan. Biotechnology Bulletin, 1, 316–319.
Rigano, L. A., Marano, M. R., Castagnaro, A. P., Do Amaral, A. M., & Vojnov, A. A. (2010). Rapid and sensitive detection of Citrus Bacterial Canker by loop-mediated isothermal amplification combined with simple visual evaluation methods. BMC Microbiology, 10, 176.
Saravanan, T., Muthusamy, M., & Marimuthu, T. (2003). Development of integrated approach to manage the fusarial wilt of banana. Crop Protection, 22, 1117–1123.
Stover, R. H. (1962). Fusarial wilt (Panama Disease) of bananas and other musa species. Kew: CMI.
Tomlinson, J., Dickinson, M., & Boonham, N. (2010). Detection of Botrytis cinerea by loop-mediated isothermal amplification. Letters in Applied Microbiology, 51, 650–657.
Tooley, P., Bunyard, B., Carras, M., & Hatziloukas, E. (1997). Development of PCR primers from internal transcribed spacer region 2 for detection of Phytophthora species infecting potatoes. Applied and Environmental Microbiology, 63, 1467–1475.
Yamazaki, W., Kumeda, Y., Misawa, N., Nakaguchi, Y., & Nishibuchi, M. (2010). Development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of the tdh and trh genes of Vibrio parahaemolyticus and related Vibrio species. Applied and Environmental Microbiology, 76, 820–828.
Zhang, Z. G., Li, Y. Q., Fan, H., Wang, Y. C., & Zheng, X. B. (2006). Molecular detection of Phytophthora capsici in infected plant tissues, soil and water. Plant Pathology, 55, 770–775.