Effects of cortisol and fluoride on ion-transporting ATPase activities in cultured osteoblastlike cells
Tóm tắt
Na+,K+-ATPase, HCO
3
−
-ATPase, Ca2+,Mg2+,-ATPase, Ca2+-ATPase, and alkaline phosphatase activities were measured in cultures of osteoblastlike cells treated with fluoride and cortisol separately and in combinations. Low concentrations of cortisol increased HCO
3
−
-ATPase (10−11 to 10−18
M cortisol) and alkaline phosphatase (10−11 to 10−9
M cortisol) activities, but higher cortisol concentrations reduced these activities. Na+,K+-ATPase, Ca2+,Mg2+-ATPase, and Ca2+-ATPase activities tended only to be reduced by cortisol. Fluoride (10−6 and 5×10−6
M) increased HCO
3
−
-ATPase and alkaline phosphatase activities, but these activities were similar to controls in the presence of 10−5
M fluoride. Ca2+,Mg2+-ATPase activity was decreased and Na+,K+-ATPase activity was increased as the concentration of fluoride increased (10−6 to 10−5
M). Preliminary experiments with fluoride indicated that lower concentrations (10−7
M) were without effect. Cortisol concentrations of 10−9 and 10−8
M were chosen for studies with combinations of cortisol and fluoride because the effects of these concentrations on alkaline phosphatase activity were opposite, i.e. 10−9
M increased whereas 10−8
M decreased activity. Fluoride concentrations of 10−6, 5×10−6, and 10−5
M were chosen because a peak of alkaline phosphatase activity occurred at 5×10−6
M fluoride. Higher (10−4
M) and lower (10−7
M) fluoride concentrations were without effect. The effects of combinations of cortisol and fluoride depend on the enzyme activity measured. Fluoride (10−6
M) combined with cortisol (10−9
M) produced a peak of Na+,K+-ATPase activity. The increased activity obtained with all concentrations of fluoride alone was preserved when fluoride was combined with 10−8
M cortisol, although the activity tended to be reduced at 5×10−6 and 10−5
M fluoride. HCO
3
−
-ATPase activity was increased by fluoride combined with 10−8
M cortisol and decreased by fluoride combined with 10−9
M cortisol compared to the activities obtained with fluoride alone. The decrease in Ca2+,Mg2+-ATPase activity caused by fluoride alone was prevented by 10−9 and enhanced by 10−8
M cortisol, although all treatments produced the same activity at 10−5
M fluoride. Ca2+-ATPase activity tended to be increased by combinations of fluoride and cortisol, but significantly so only at 10−5
M fluoride in combinations with 10−8 and 10−9
M cortisol. Alkaline phosphatase activity was increased by fluoride combined with 10−9
M cortisol and decreased by fluoride combined with 10−8
M cortisol compared to the activities obtained with fluoride alone. These results suggest that the abilities of bone cells to regulate ion transport (as reflected in their ion-transporting ATPase activities) are modulated by glucocorticoids and fluoride. Inasmuch as these cells may regulate the ionic composition and concentrations of the bone extracellular fluid (ECF) in vivo, the modulation of their activities by cortisol and fluoride may result in altered bone ECF composition.
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