Use of a recombinant antigen, SAG2, expressed as a glutathione-S-transferase fusion protein to immunize mice against Toxoplasma gondii

Parasitology Research - Tập 83 - Trang 6-9 - 1996
A. Lundén1, S. F. Parmley2, K. Lövgren Bengtsson3, F. G. Araujo2
1Department of Parasitology, National Veterinary Institute, PO Box 7073, S-750 07 Uppsala, Sweden Fax: +46-18 309162 e-mail: [email protected], , SE
2Department of Immunology and Infectious Diseases, Research Institute, Palo Alto Medical Foundation, Palo Alto, CA, USA, , US
3Department of Virology, Swedish University of Agricultural Sciences and National Veterinary Institute, Uppsala, Sweden, , SE

Tóm tắt

 The capacity of Toxoplasma gondii surface protein SAG2 to induce protective immunity against the parasite in mice was studied using recombinant SAG2 expressed as a glutathione-S-transferase (GST) fusion protein incorporated into immune stimulating complexes (iscoms). Immunization with the iscoms resulted in the production of antibodies recognizing SAG2 as well as GST. After oral challenge infection with T. gondii oocysts or tissue cysts, no protective effect was observed. On the contrary, mice immunized with fusion SAG2 or with GST iscoms died earlier than non-immunized control mice.

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Parasitology Research

Tập 83

6-9

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