Angiotensinogen gene expression in extrahepatic rat tissues: Application of a solution hybridization assay
Naunyn-Schmiedebergs Archiv für Pharmakologie und experimentelle Pathologie - Tập 338 - Trang 327-331 - 1988
Tóm tắt
Angiotensin II has numerous biological effects in a hitherto unsuspected variety of tissues. The generation of angiotensin in tissue requires the local presence of its high molecular weight precursor angiotensinogen and is best tested by investigating angiotensinogen gene expression. A quantitative solution hybridization assay for rapid and sensitive measurement of angiotensinogen mRNA was therefore established to study the extrahepatic expression of the angiotensinogen gene. We used a 714 bases BamHI angiotensinogen cDNA fragment cloned into vector pSPT18 and developed a sensitive and rapid assay with a detection limit of 0.5 pg RNA. Quantification of angiotensinogen mRNA from male Sprague-Dawley rats resulted in the following tissue levels (n = 10 for all tissues, except pituitary where n = 5), was expressed as fg mRNA per jig total RNA, in descending order: liver (9950), hypothalamus (6050), midbrain (4450), brainstem (3950), total brain (2325), aorta (625), kidney (338), adrenal gland (170), and heart atrium (140). The high sensitivity of the assay in addition also allowed for the first time measurement of angiotensinogen mRNA in the low gene expression tissues pituitary (70), heart ventricle (30), and testis (30). This assay will allow detailed studies on the regulation of tissue angiotensinogen and the pathophysiological role of the tissue renin angiotensin systems.
Tài liệu tham khảo
Auffray C, Rougeon F (1980) Purification of mouse immunoglobulin heavy chain mRNAs from total myeloma tumor RNA. Eur J Biochem 107:303–314
Campbell DJ (1985) The site of angiotensin production. J Hypertension 3:199–207
Campbell DJ (1987) Circulating and tissue angiotensin systems. J Clin Invest 79:1–6
Campbell DJ, Habener F (1986) Angiotensinogen gene is expressed and differently regulated in multiple tissues of the rat. J Clin Invest 78:31–39
Campbell DJ, Habener JF (1987) Cellular localization of angiotensinogen gene expression in brown adipose tissue and mesentery: quantification of messenger ribonucleic acid abundance using hybridization in situ. Endocrinology 121:1616–1626
Campbell DJ, Bouhnik J, Menard J, Corvol P (1984) Identity of angiotensinogen precursors of rat brain and liver. Nature 308:206–208
Durnam DM, Palmiter RD (1983) A practical approach for quantitating specific mRNAs by solution hybridization assay. Analyt Biochem 131:385–393
Dzau VJ, Ingelfinger J, Pratt RE, Ellison KE (1986) Identification of renin and angiotensinogen messenger RNA sequences in mouse and rat brains. Hypertension 8:544–548
Fischer-Ferraro C, Nahmod VW, Goldstein DJ, Finkielman S (1971) Angiotensin and renin in rat and dog brain. J Exp Med 133:353–361
Ganten D, Minnich JL, Granger P, Hayduck K, Brecht HM, Barbean A, Karsunky KP, Boucher R (1971) Genest J. Angiotensin-forming enzyme in brain tissue. Science 173:64–65
Le Meur M, Glanville M, Mandel JL, Gerlinger P, Palmitier R, Chambon P (1981) The ovalbumin gene family: hormonal control of x and y gene transcription and mRNA accumulation. Cell 23:561–571
Lynch KR, Simnad I, Ben-Ari ET, Garrison JC (1986) Localization of preangiotensinogen messenger RNA sequences in the rat brain. Hypertension 8:540–543
Maniatis T, Fritsch EF, Sambrook J (1982) Selection of poly(A)+ RNA. In: Maniatis T, Fritsch EF, Sambrook J (eds) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, New York, pp 197–198
McKnight G, Schimke RT (1974) Ovalbumin messenger RNA: Evidence that the initial product of transcription is the same size as polysomal ovalbumin messenger. Proc Natl Acad Sci USA 71:4327–4331
Melton DA, Krieg PA, Rebagliati MR, Maniatis T, Zinn K, Green MR (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage sp6 promoter. Nucleic Acids Res 12:7053–7056
Morris BJ (1986) New possibilities for intracellular renin and inactive renin now that the structure of the human renin gene has been established. Clinical Science 71:345–355
Naruse M, Naruse K, McKenzie JC, Schelling P, Inagami T (1985) Regional distribution of renin and angiotensinogen in the brain of normotensive (WKY) and spontaneously hypertensive (SHR) rats. Brain Res 333:147–150
Nasjletti A, Masson GMC (1971) Hepatic origin of renin substrate. Can J Physiol Pharmacol 49:931–932
Ohkubo H, Kageyama R, Ujihara M, Inayama S, Nakanishi S (1983) Cloning and sequence analysis of cDNA of rat angiotensinogen. Proc Natl Acad Sci USA 80:2196–2200
Panthier JJ, Foote S, Chambraud B, Strosberg AD, Corvol P, Rougeon F (1982) Complete amino acid sequence and maturation of the mouse submaxillary gland renin precursor. Nature 298:90–92
Paul M, Wagner D, Metzger R, Ganten D, Lang RE, Suzuki F, Murakami K, Burbach JHP, Ludwig G (1988) Quantification of renin mRNA in various mouse tissues by a novel solution hybridization assay. J Hypertension 6:247–252
Reid IA, Morris BJ, Ganong WF (1978) The renin angiotensin system. Ann Rev Physiol 40:377–410
Suzuki F, Ludwig G, Hellmann W, Paul M, Lindpaintner K, Murakami K, Ganten D (1988) Renin gene expression in rat tissue: a new quantitative assay method for rat renin mRNA using synthetic cRNA. Clin Exp Hypertension 2:345–359
Vescei P, Hackenthal E, Ganten D (1978) The renin-angiotensin-system: past, present, future. Klin Wochenschr 56 (Suppl I): 5–21