Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes
Tóm tắt
The influence of donor plant growth conditions in years 2003, 2004, and 2005 on tissue culture response (TCR) traits of 96 wheat genotypes was evaluated. Immature embryos, collected 12–15 days after anthesis from field-grown plants, were cultured on Murashige and Skoog medium containing 2 mg/l 2,4-D followed by their transfer to a growth regulator-free medium. Donor plants growth in a season 2003 characterized by drought and heat stress, as compared to favorable growth seasons 2004 and 2005, resulted in a increased variability and a decreased percentage of callus formation and the number of regenerating calli and plants regenerated per embryo in all genotypes, except genotype Florida that exhibited a significantly increased number of regenerating calli in 2003. The reduction rate of regenerating callus formation depended on genotype, and it was the highest in Magnif 41, NS66/92, and Mexico 3. The results suggest that the impact of donor plant growth conditions on TCR traits can be as large as the effect of the genotype, which should be taken into account when planning transformation work with wheat.
Tài liệu tham khảo
Maheshwari, N., Rajyalakshmi, K., Baweja, K., Dhir, S.K., Chowdhry, C.N., and Maheshwari, S.C., In Vitro Culture of Wheat and Genetic Transformation — Retrospect and Prospect, Crit. Rev. Plant Sci., 1995, vol. 14, pp. 149–178.
Maés, O., Chibbar, R., Caswell, K., Leung, N., and Kartha, K., Somatic Embryogenesis from Isolated Scutella of Wheat: Effect of Physical, Physiological and Genetic Factors, Plant Sci., 1996, vol. 121, pp. 75–84.
Machii, H., Mizuno, H., Hirabayashi, T., Li, H., and Hagio, T., Screening Wheat Genotypes for High Callus Induction and Regeneration Capability from Anther and Immature Embryo Cultures, Plant Cell, Tissue Organ Cult., 1998, vol. 53, pp. 67–74.
Gonzälez, J.M., Friero, E., and Jouve, N., Influence of Genotype and Culture Medium on Callus Formation and Plant Regeneration from Immature Embryos of Triticum turgidum Desf. Cultivars, Plant Breed., 2001, vol. 120, pp. 513–517.
Hess, J. and Carman, J., Competence of Immature Wheat Embryos: Genotype, Donor Plant Environment and Endogenous Hormone Levels, Crop Sci., 1998, vol. 38, pp. 249–253.
Kobiljski, B., Quarrie, S.A., Denćić, S., Kirby, J., and Ivegeš, M., Genetic Diversity of the Novi Sad Wheat Core Collection Revealed by Microsatellites, Cell. Mol. Biol. Lett., 2002, vol. 7, pp. 685–694.
Murashige, T. and Skoog, F., A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures, Physiol. Plant., 1962, vol. 15, pp. 473–497.
Kopertekh, L.G. and Butenko, R.G., Naturally Occuring Phytohormones in Wheat Explants as Related to Wheat Morphogenesis In Vitro, Russ. J. Plant Physiol., 1995, vol. 42, pp. 488–491.
Jiménez, V. and Bangerth, F., Endogenous Hormone Concentrations and Embryogenic Callus Development in Wheat, Plant Cell, Tissue Organ Cult., 2001, vol. 67, pp. 37–46.
Cai, T.S., Tian, H.Q., Lin, S.K., and Li, J.M., Effects of Genotype and Embryo Age on Wheat Immature Embryo Response in In Vitro Culture, Acta Genet. Sinica, 1989, vol. 16, pp. 81–88.
Özgen, M., Türet, M., Özcan, S., and Sancak, C., Callus Induction and Plant Regeneration from Immature and Mature Embryos of Winter Durum Wheat Genotypes, Plant Breed., 1996, vol. 115, pp. 455–458.
Wu, B.H., Zheng, Y.L., Liu, D.C., and Zhou, Y.H., Trait Correlation of Immature Embryo Culture in Bread Wheat, Plant Breed., 2003, vol. 122, pp. 47–51.
Jones, A.M. and Petolino, J.F., Effects of Donor Plant Genotype and Growth Environment on Anther Culture of Soft-Red Winter Wheat (Triticum aestivum L.), Plant Cell, Tissue Organ Cult., 1987, vol. 8, pp. 215–223.
Lu, C.S., Sharma, H.C., and Ohm, H.W., Wheat Anther Culture: Effect of Genotype and Environmental Conditions, Plant Cell, Tissue Organ Cult., 1991, vol. 24, pp. 233–236.
Orshinsky, B.R. and Sadasivaiah, R.S., Effect of Plant Growth Conditions, Plating Density, and Genotype on Anther Culture Response of Soft White Spring Wheat Hybrids, Plant Cell Rep., 1997, vol. 16, pp. 758–762.
Lazar, M.D., Collins, G.B., and Vian, W.E., Genetic and Environmental Effects on the Growth and Differentiation of Wheat Somatic Cell Cultures, J. Hered., 1983, vol. 74, pp. 353–357.
Carman, J.G., Somatic Embryogenesis in Wheat: Biotechnology in Agriculture and Forestry, Somatic Embryogenesis in Wheat, Bajaj, Y.P.S., Ed., Heidelberg: Springer-Verlag, 1995, pp. 3–13.