A<i>trans</i>‐acting factor, isolated by the three‐hybrid system, that influences alternative splicing of the amyloid precursor protein minigene

FEBS Journal - Tập 267 Số 13 - Trang 4002-4010 - 2000
Andrej Poleev1, Annette M. Hartmann1, Stefan Stamm1
1Max-Planck-Institute for Neurobiology, Munich, Germany

Tóm tắt

Two clones were isolated in a three‐hybrid screen of a rat fetal brain P5 cDNA library with an intronic splicing enhancer of the amyloid precursor protein (APP) gene as RNA bait. These clones represent the rat homologues of the previously described genes CUG‐binding protein (CUG‐BP) and Siah‐binding protein (Siah‐BP). Both interact in a sequence‐specific manner with the RNA bait used for library screening as well as with the CUG repeat. In contrast, no interactions were observed in the three‐hybrid assay with other baits tested. In two‐hybrid assays, Siah‐BP interacts with U2AF65 as well as with itself. EWS, an RGG‐type RNA‐binding protein associated with Ewing sarcoma, was identified as an interacting partner for the CUG‐BP homologue in a two‐hybrid assay for protein–protein interactions performed with various factors involved in RNA metabolism. Splicing assays performed by RT‐PCR from cells cotransfected with certain cDNAs and an APP minigene, used as a reporter, indicate exclusion of exon 8 if the CUG‐BP homologue is present. We conclude that clone AF169013 and its counterpart in human CUG‐BP could be thetrans‐acting factors that interact with the splicing enhancer downstream of exon 8, and in this way influence alternative splicing of the APP minigene.

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FEBS Journal

Tập 267 Số 13

4002-4010

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