Biomaterials based on chitin and chitosan in wound dressing applications

Biotechnology Advances - Tập 29 - Trang 322-337 - 2011
R. Jayakumar1, M. Prabaharan2, P.T. Sudheesh Kumar1, S.V. Nair1, H. Tamura3
1Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidhyapeetham University, Cochin-682 041, India
2Department of Chemistry, Faculty of Engineering and Technology, SRM University, Kattankulathur-603 203, India
3Faculty of Chemistry, Materials and Bioengineering, Kansai University, Osaka-564-8680, Japan

Tài liệu tham khảo

Anitha, 2009, Synthesis, characterization, cytotoxicity and antibacterial studies of chitosan, O-carboxymethyl and N, O-carboxymethyl chitosan nanoparticles, Carbohydr Polym, 78, 672, 10.1016/j.carbpol.2009.05.028 Anitha, 2010, Development of mucoadhesive thiolated chitosan nanoparticles for biomedical applications, Carbohydr Polym Aoyagi, 2007, Novel chitosan wound dressing loaded with minocycline for the treatment of severe burn wounds, Int J Pharm, 330, 138, 10.1016/j.ijpharm.2006.09.016 Azad, 2004, Chitosan membrane as a wound-healing dressing: characterization and clinical application, J Biomed Mater Res Appl Biomater, 69B, 216, 10.1002/jbm.b.30000 Balassa, 1984, Applications of chitin and chitosan in wound healing acceleration, 296 Berthod, 1996, Deposition of collagen fibril bundles by long-term culture of fibroblasts in a collagen sponge, J Biomed Mater Res, 32, 87, 10.1002/(SICI)1097-4636(199609)32:1<87::AID-JBM10>3.0.CO;2-F Blasinska, 2008, Effects of nonwoven mats of di-O-butyrylchitin and related polymers on the process of wound healing, Biomacroloecules, 9, 776, 10.1021/bm7006373 1999 Boucard, 2007, The use of physical hydrogels of chitosan for skin regeneration following third-degree burns, Biomaterials, 28, 3478, 10.1016/j.biomaterials.2007.04.021 Boyce, 1988, Structure of a collagen-GAG dermal skin substitute optimized for cultured human epidermal keratinocytes, J Biomed Mater Res, 22, 939, 10.1002/jbm.820221008 Brown, 2009, Experience with chitosan dressings in a civilian EMS system, J Emerg Med, 37, 1, 10.1016/j.jemermed.2007.05.043 Bruin, 1990, A new porous polyetherurethane wound covering, J Biomed Mater Res, 24, 217, 10.1002/jbm.820240208 Burkatovskaya, 2008, Effect of chitosan acetate bandage on wound healing in infected and noninfected wounds in mice, Wound Repair Regen, 16, 425, 10.1111/j.1524-475X.2008.00382.x Cai, 2010, Fabrication of chitosan/silk fibroin composite nanofibers for wound-dressing applications, Int J Mol Sci, 11, 3529, 10.3390/ijms11093529 Casimiro, 2010, Suitability of gamma irradiated chitosan based membranes as matrix in drug release system, Int J Pharm, 395, 142, 10.1016/j.ijpharm.2010.05.034 Chang, 1988, Polymeric templates for peripheral nerve regeneration. Electrophysiological study of functional recovery, 59, 906 Chen, 2006, Development of N, O-(carboxymethyl)chitosan/collagen matrixes as a wound dressing, Biomacromolecules, 7, 1058, 10.1021/bm050754b Chen, 2008, Intermolecular interactions in electrospun collagen-chitosan complex nanofibers, Carbohydr Polym, 72, 410, 10.1016/j.carbpol.2007.09.018 Chilarski, 2007, Novel dressing materials accelerating wound healing made from dibutyrylchitin. Fibers, Tex Eas Eur, 15, 77 Cho, 1999, Water-soluble chitin as a wound healing accelerator, Biomaterials, 20, 2139, 10.1016/S0142-9612(99)00116-7 Clasen, 2006, Formation and characterization of chitosan membranes, Biomacromolecules, 7, 3210, 10.1021/bm060486x Cohen, 2000, The theory of real materials, Annu Rev Mater Sci, 30, 1, 10.1146/annurev.matsci.30.1.1 Dagalakis, 1980, Design of an artificial skin. III. Control of pore structure, J Biomed Mater Res, 14, 511, 10.1002/jbm.820140417 Dagalakis, 1980, Design of an artificial skin. Part III. Control of pore structure, J Biomed Mater Res, 14, 511, 10.1002/jbm.820140417 Denkbas, 2004, Norfloxacin-loaded chitosan sponges as wound dressing material, J Biomater Appl, 18, 291, 10.1177/0885328204041510 Dev, 2010, Novel carboxymethyl chitin nanoparticles for cancer drug delivery applications, Carbohydr Polym, 79, 273, 10.1016/j.carbpol.2009.10.038 Dong, 2010, A novel CHS/ALG bi-layer composite membrane with sustained antimicrobial efficacy used as wound dressing, Chin Chem Lett, 21, 1011, 10.1016/j.cclet.2010.04.010 Draye, 1998, In vitro and in vivo biocompatibility of dextran dialdehyde cross-linked gelatin hydrogel films, Biomaterials, 19, 1677, 10.1016/S0142-9612(98)00049-0 Haitang, 2007, Chitosan-hyaluronic acid hybrid film as a novel wound dressing: in vitro and in vivo studies, Polym Adv Technol, 18, 869, 10.1002/pat.906 Han, 2005, Topical formulations of water-soluble chitin as a wound healing assistant-evaluation on open wounds using a rabbit ear model, Fibers Polym, 6, 219, 10.1007/BF02875645 Hansbrough, 1989, Burn wound closure with cultured autologous keratinocytes and fibroblasts attached to a collagen-glycosaminoglycan substrate, J Am Med Assoc, 262, 2125, 10.1001/jama.1989.03430150093032 Hinrichs, 1992, Fibraction and characterization of an asymmetric polyurethane membrane for use as a wound dressing, J Appl Biomater, 3, 287, 10.1002/jab.770030408 Hoffman, 2002, Hydrogels for biomedical applications, Adv Drug Deliv Rev, 43, 3, 10.1016/S0169-409X(01)00239-3 Hong, 2008, Accelerated wound healing by smad3 antisense oligonucleotides-impregnated chitosan/alginate polyelectrolyte complex, Biomaterials, 29, 4831, 10.1016/j.biomaterials.2008.08.023 Huang, 2003, A review on polymer nanofibers by electrospinning and their applications in nanocomposites, Compos Sci Technol, 63, 2223, 10.1016/S0266-3538(03)00178-7 Ignatova, 2006, Electrospun nano-fibre mats with antibacterial properties from quaternized chitosan and poly(vinyl alcohol), Carbohydr Res, 341, 2098, 10.1016/j.carres.2006.05.006 Ignatova, 2007, Novel antibacterial fibers of quaternized chitosan and poly(vinyl pyrrolidine) prepared by electrospinning, Eur Polym J, 43, 1112, 10.1016/j.eurpolymj.2007.01.012 Ishihara, 2001, Acceleration of wound contraction and healing with a photocrosslinkable chitosan hydrogel, Wound Repair Regen, 9, 513, 10.1046/j.1524-475x.2001.00513.x Ishihara, 2002, Photocrosslinkable chitosan as a dressing for wound occlusion and accelerator in healing process, Biomaterials, 23, 833, 10.1016/S0142-9612(01)00189-2 Jayakumar, 2005, Graft copolymerized chitosan — present status and applications, Carbohydr Polym, 62, 142, 10.1016/j.carbpol.2005.07.017 Jayakumar, 2006, Phosphorous containing chitosan beads for controlled oral drug delivery, J Bioact Compat Polym, 21, 327, 10.1177/0883911506066929 Jayakumar, 2007, Sulfated chitin and chitosan as novel biomaterials, Int J Biol Macromol, 40, 175, 10.1016/j.ijbiomac.2006.06.021 Jayakumar, 2008, Synthesis, characterization and biospecific degradation behavior of sulfated chitin, Macromol Symp, 264, 163, 10.1002/masy.200850426 Jayakumar, 2009, Bioactive and osteoblast cell attachement studies of novel α-, and β-chitin membranes for tissue engineering applications, Int J Biol Macromol, 45, 260, 10.1016/j.ijbiomac.2009.06.002 Jayakumar, 2010, Chitosan conjugated DNA nanoparticles in gene therapy, Carbohydr Polym, 79, 1, 10.1016/j.carbpol.2009.08.026 Jayakumar, 2010, Biomedical applications of chitin and chitosan nanomaterials — a short review, Carbohydr Polym, 82, 227, 10.1016/j.carbpol.2010.04.074 Jayakumar, 2010, Novel chitin and chitosan nanofibers in biomedical applications, Biotechnol Adv, 28, 142, 10.1016/j.biotechadv.2009.11.001 Jayakumar, 2010, Novel carboxymethyl derivatives of chitin and chitosan materials and their biomedical applications, Prog Mater Sci, 55, 675, 10.1016/j.pmatsci.2010.03.001 Jayakumar R, Sudhessh Kumar PT, Abhilash S, Manzoor K, Lakshmanan VK, Nair SV. The art, method, manner, process and system of chitosan hydrogel/nano zinc oxide membranes for wound dressing applications. Indian Patent, File. No. 1025/CHE/2010. Jing, 2010, Preparation of chitosan-graft-(methyl methacrylate)/Ag nanocomposite with antimicrobial activity, Polym Int, 59, 62, 10.1002/pi.2689 Kim, 2008, Chitosan and its derivatives for tissue engineering applications, Biotechnol Adv, 26, 1, 10.1016/j.biotechadv.2007.07.009 Kofuji, 2010, Preparation and evaluation of a novel wound dressing sheet comprised of β-glucan-chitosan complex, React Funct Polym, 70, 784, 10.1016/j.reactfunctpolym.2010.07.014 Kossovich, 2010, Electrospun chitosan nanofiber materials as burn dressing, IFMBE Proc, 31, 1212, 10.1007/978-3-642-14515-5_307 Kweon, 2003, Preparation of water-soluble chitosan/heparin complex and its application as wound healing accelerator, Biomaterials, 24, 1595, 10.1016/S0142-9612(02)00566-5 Lee, 2000, Beta-chitin-based wound dressing containing silver sulfurdiazine, J Mater Sci Mater Med, 11, 817, 10.1023/A:1008961730929 Lee, 2009, Advances in chitosan material and its hybrid derivatives: a review, Open Biomater J, 1, 10, 10.2174/1876502500901010010 Li, 2010, Preparation, characterization and antimicrobial activities of chitosan/Ag/ZnO blend films, Chem Eng J, 160, 378, 10.1016/j.cej.2010.03.051 Liu, 2007, Release of basic fibroblast growth factor from a crosslinked glycosaminoglycan hydrogel promotes wound healing, Wound Repair Regen, 15, 245, 10.1111/j.1524-475X.2007.00211.x Liu, 2008, Evaluation of a non-woven fabric coated with a chitosan bi-layer composite for wound dressing, Macromol Biosci, 8, 432, 10.1002/mabi.200700211 Loke, 2000, Wound dressing with sustained anti-microbial capability, J Biomed Mater Res Appl Biomater, 53, 8, 10.1002/(SICI)1097-4636(2000)53:1<8::AID-JBM2>3.0.CO;2-3 Lu, 2008, Construction, application and biosafety of silver nanocrystalline chitosan wound dressing, Burns, 34, 623, 10.1016/j.burns.2007.08.020 Lu, 2010, A novel in situ-formed hydrogel wound dressing by the photocross-linking of a chitosan derivative, Wound Repair Regen, 18, 70, 10.1111/j.1524-475X.2009.00557.x Luo, 2005, The role of poly(ethylene glycol) in the formation of silver nanoparticles, J Colloid Interface Sci, 288, 444, 10.1016/j.jcis.2005.03.005 Madhumathi, 2009, Preparation and characterization of novel_α-chitin-hydroxyapatite composite membranes for tissue engineering applications, Int J Biol Macromol, 44, 1, 10.1016/j.ijbiomac.2008.09.013 Madhumathi, 2010, Development of novel chitin/nanosilver composite scaffolds for wound dressing applications, J Mater Sci Mater Med, 21, 807, 10.1007/s10856-009-3877-z Maeda, 2008, Synthesis, characterization and bioactivity studies of novel β-chitin scaffolds for tissue-engineering applications, Int J Biol Macromol, 42, 463, 10.1016/j.ijbiomac.2008.03.002 Marreco, 2004, Effects of different sterilization methods on the morphology, mechanical properties and cytotoxicity of chitosan membranes used as wound dressings, J Biomed Mater Res B Appl Biomater, 71B, 268, 10.1002/jbm.b.30081 Matsuda, 1990, Influence of glycosaminoglycans on the collagen sponges component of a bilayer artificial skin, Biomaterials, 11, 351, 10.1016/0142-9612(90)90113-5 Matsuda, 1990, Influence of glycosaminoglycanos on the collagen sponge component of a bilayer artificial skin, Biomaterials, 1, 351, 10.1016/0142-9612(90)90113-5 Matsuda, 1992, Evaluation of bilayer artificial skin capable of sustained release of an antibiotic, Biomaterials, 13, 119, 10.1016/0142-9612(92)90007-B Matsuda, 1993, Re-freeze dried bilayer artificial skin, Biomaterials, 14, 1030, 10.1016/0142-9612(93)90197-A Mattioli-Belmonte, 2007, Chitin nanofibrils linked to chitosan glycolate as spray, gel and gauze preparations for wound repair, J Bioact Comp Polym, 22, 525, 10.1177/0883911507082157 Mi, 2001, Fabrication and characterization of a sponge-like asymmetric chitosan membrane as a wound dressing, Biomaterials, 22, 165, 10.1016/S0142-9612(00)00167-8 Mi, 2003, Asymmetric chitosan membranes prepared by dry/wet phase separation: a new type of wound dressing for controlled antibacterial release, J Membr Sci, 212, 237, 10.1016/S0376-7388(02)00505-7 Mizuno, 2003, Effect of chitosan film containing basic fibroblast growth factor on wound healing in genetically diabetic mice, J Biomed Mater Res, 64A, 177, 10.1002/jbm.a.10396 Murakami, 2010, Hydrogel blends of chitin/chitosan, fucoidan and alginate as healing-impaired wound dressings, Biomaterials, 31, 83, 10.1016/j.biomaterials.2009.09.031 Muramatsu, 2003, In vitro degradation behavior of freeze-dried carboxymethyl-chitin sponges processed by vacuum-heating and gamma irradiation, Polym Deg Stab, 81, 327, 10.1016/S0141-3910(03)00103-4 Muzzarelli, 1993, Biochemical significance of exogenous chitins and chitosans in animals and patients, Carbohydr Polym, 20, 7, 10.1016/0144-8617(93)90027-2 Muzzarelli, 2009, Chitin and chitosans for the repair of wounded skin, nerve, cartilage and bone, Carbohydr Polym, 76, 167, 10.1016/j.carbpol.2008.11.002 Muzzarelli, 2005, The biocompatibility of dibutyryl chitin in the context of wound dressings, Biomaterials, 26, 5844, 10.1016/j.biomaterials.2005.03.006 Muzzarelli, 2007, Chitin nanofibrils/chitosan glycolate composites as wound medicaments, Carbohydr Polym, 70, 274, 10.1016/j.carbpol.2007.04.008 Nagahama, 2008, Preparation of biodegradable chitin/gelatin membranes with GlcNAc for tissue engineering applications, Carbohydr Polym, 73, 456, 10.1016/j.carbpol.2007.12.011 Nagahama, 2008, Novel biodegradable chitin membranes for tissue engineering applications, Carbohydr Polym, 73, 295, 10.1016/j.carbpol.2007.11.034 Nagahama, 2009, Preparation, characterization, bioactive and cell attachment studies of α-chitin/gelatin composite membranes, Int J Biol Macromol, 44, 333, 10.1016/j.ijbiomac.2009.01.006 O'Connor, 1981, Grafting of burns with cultured epithelium prepared from autologous epidermal cells, Lancet, 317, 75, 10.1016/S0140-6736(81)90006-4 Ong, 2008, Development of a chitosan-based wound dressing with improved hemostatic and antimicrobial properties, Biomaterials, 29, 4323, 10.1016/j.biomaterials.2008.07.034 Paul, 2004, Chitin and alginates wound dressings: a short review, Trends Biomater Artif Organs, 18, 18 Pei, 2008, Characterization and ornidazole release in vitro of a novel composite film prepared with chitosan/poly(vinyl alcohol)/alginate, J Biomed Mater Res, 85A, 566, 10.1002/jbm.a.31223 Peter, 2009, Development of novel chitin/nano bioactive glass ceramic nanocomposite scaffolds for tissue engineering applications, Carbohydr Polym, 78, 926, 10.1016/j.carbpol.2009.07.016 Peter, 2010, Nanocomposite scaffolds of bioactive glass ceramic nanoparticles disseminated chitosan matrix for tissue engineering applications, Carbohydr Polym, 79, 284, 10.1016/j.carbpol.2009.08.001 Pietramaggiori, 2008, Effects of poly-N-acetyl glucosamine (pGlcNAc) patch on wound healing in db/db mouse, J Trauma, 64, 803, 10.1097/01.ta.0000244382.13937.a8 Portero, 2007, Development of chitosan sponges for buccal administration of insulin, Carbohydr Polym, 68, 617, 10.1016/j.carbpol.2006.07.028 Prabaharan, 2008, Chitosan derivatives as promising materials for controlled drug delivery, J Biomater Appl, 23, 5, 10.1177/0885328208091562 Prabaharan, 2009, Chitosan-graft-β-cyclodextrin scaffolds with controlled drug release capability for tissue engineering applications, Int J Biol Macromol, 44, 320, 10.1016/j.ijbiomac.2009.01.005 Prabaharan, 2005, Chitosan-based particles as controlled drug delivery systems, Drug Deliv, 12, 41, 10.1080/10717540590889781 Radhakumarya, 2011, Drug loaded thermoresponsive and cytocompatible chitosan based hydrogel as a potential wound dressing, Carbohydr Polym, 83, 705, 10.1016/j.carbpol.2010.08.042 Rai, 2009, Silver nanoparticles as a new generation of antimicrobials, Biotechnol Adv, 27, 76, 10.1016/j.biotechadv.2008.09.002 Rasad, 2010, In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts, Carbohydr Polym, 79, 1094, 10.1016/j.carbpol.2009.10.048 Ravi Kumar, 2000, A review of chitin and chitosan applications, React Funct Polym, 46, 1, 10.1016/S1381-5148(00)00038-9 Ribeiro, 2009, Development of a new chitosan hydrogel for wound dressing, Wound Repair Regen, 17, 817, 10.1111/j.1524-475X.2009.00538.x Rodrigues, 2008, The influence of preparation conditions on the characteristics of chitosan-alginate dressings for skin lesions, J Appl Polym Sci, 109, 2703, 10.1002/app.28203 Sachlos, 2003, Making tissue engineering scaffolds work. Review on the application of solid freeform fabrication technology to the production of tissue engineering scaffolds, Eur Cells Mater, 5, 29, 10.22203/eCM.v005a03 Shalumon, 2009, Electrospinning of carboxymethyl chitin/poly(vinyl alcohol) nanofibrous scaffolds for tissue engineering applications, Carbohydr Polym, 77, 863, 10.1016/j.carbpol.2009.03.009 Shalumon, 2010, Single step electrospinning of chitosan/poly(caprolactone) nanofibers using formic acid/acetone solvent mixture, Carbohydr polym, 80, 413, 10.1016/j.carbpol.2009.11.039 Sharma, 1995, Photocatalytic reactions of xylidine ponceau on semiconducting zinc oxide powder, J Photochem Photobiol, A86, 197, 10.1016/1010-6030(94)03933-L Shi, 2006, Therapeutic potential of chitosan and its derivatives in regenerative medicine, J Surg Res, 133, 185, 10.1016/j.jss.2005.12.013 Shibata, 1997, Development of new wound dressing composed of spongy collagen sheet containing dibutyryl cyclic AMP, J Biomater Sci Polym Ed, 8, 601, 10.1163/156856297X00209 Silva, 2008, Plasma surface modification of chitosan membranes: characterization and preliminary cell response studies, Macromol Biosci, 8, 568, 10.1002/mabi.200700264 Sudheesh Kumar, 2010, Preparation and characterization of novel β-chitin/nano silver composite scaffolds for wound dressing applications, Carbohydr Polym, 80, 761, 10.1016/j.carbpol.2009.12.024 Sung, 2010, Gel characterization and in vivo evaluation of minocycline-loaded wound dressing with enhanced wound healing using polyvinyl alcohol and chitosan, Int J Pharm, 392, 232, 10.1016/j.ijpharm.2010.03.024 Suzuki, 1990, Experimental study of newly developed bilayer artificial skin, Biomaterials, 11, 356, 10.1016/0142-9612(90)90114-6 Tamura, 2006, Preparation of chitin hydrogel under mild conditions, Cellulose, 13, 357, 10.1007/s10570-006-9058-z Tamura, 2010, Biomedical applications of chitin hydrogel membranes and scaffolds, Carbohydr Polym Tanodekaew, 2004, Preparation of acrylic grafted chitin for wound dressing application, Biomaterials, 25, 1453, 10.1016/j.biomaterials.2003.08.020 Tashiro, 2001, Antibacterial and bacterium adsorbing macromolecules, Macromol Mater Eng, 286, 63, 10.1002/1439-2054(20010201)286:2<63::AID-MAME63>3.0.CO;2-H Teo, 2009, Electrospun nanofibers as a platform for multifunctional, hierarchically organized nanocomposite, Compos Sci Technol, 69, 1804, 10.1016/j.compscitech.2009.04.015 Thomas, 2009, Fabrication, characterization of chitosan/nanosilver film and its potential antibacterial application, J Biomater Sci Polym Ed, 20, 2129, 10.1163/156856209X410102 Tsao, 2010, Evaluation of chitosan/γ-poly(glutamic acid) polyelectrolyte complex for wound dressing materials, Carbohydr Polym 1986 Ueno, 2001, Topical formulations and wound healing applications of chitosan, Adv Drug Deliv Rev, 52, 105, 10.1016/S0169-409X(01)00189-2 Ulubayram, 2001, EGF containing gelatin-based wound dressings, Biomaterials, 22, 1345, 10.1016/S0142-9612(00)00287-8 Vicentini, 2010, Chitosan/poly (vinyl alcohol) films containing ZnO nanoparticles and plasticizers, Mater Sci Eng, C30, 503, 10.1016/j.msec.2009.01.026 Vimala, 2010, Fabrication of porous chitosan films impregnated with silver nanoparticles: a facile approach for superior antibacterial application, Colloids Surf B Biointerfaces, 76, 248, 10.1016/j.colsurfb.2009.10.044 Wang, 2004, Zinc oxide nanostructures: growth, properties and applications, J Phys Condens Matter, 16, R829, 10.1088/0953-8984/16/25/R01 Wang, 2008, Water absorbing and antibacterial properties of N-isopropyl acrylamide grafted and collagen/chitosan immobilized polypropylene nonwoven fabric and its application on wound healing enhancement, J Biomed Mater Res, 84A, 1006, 10.1002/jbm.a.31482 Watthanaphanit, 2008, Fabrication, structure, and properties of chitin whisker-reinforced alginate nanocomposite fibers, J Appl Polym Sci, 110, 890, 10.1002/app.28634 1999 Xu, 2007, Chitosan-hyaluronic acid hybrid film as a novel wound dressing: in vitro and in vivo studies, Polym Adv Technol, 18, 869, 10.1002/pat.906 Yang, 2010, Cytotoxicity and wound healing properties of PVA/ws-chitosan/glycerol hydrogels made by irradiation followed by freeze-thawing, Radiat Phys Chem, 79, 606, 10.1016/j.radphyschem.2009.12.017 Yannas, 1980, Design of an artificial skin. I. Basic design principles, J Biomed Mater Res, 14, 65, 10.1002/jbm.820140108 Yannas, 1980, Design of an artificial skin. II. Control of chemical composition, J Biomed Mater Res, 14, 107, 10.1002/jbm.820140203 Yannas, 1981, Prompt long-term functional replacement of skin, Trans Am Sot Artif Int Organs, 27, 19 Yusof, 2001, Preparation and characterization of chitin beads as a wound dressing precursor flexible chitin films as potential wound-dressing materials: wound model studies, J Biomed Mater Res, 54, 59, 10.1002/1097-4636(200101)54:1<59::AID-JBM7>3.0.CO;2-U Yusof, 2003, Flexible chitin films as potential wound-dressing materials: wound model studies, J Biomed Mater Res, 66A, 224, 10.1002/jbm.a.10545 Zhang, 2007, Biomimetic and bioactive nanofi brous scaffolds from electrospun composite nanofibers, Int J Nanomedicine, 2, 623 Zhang, 2008, Chitosan/polyethylene glycol diacrylate films as potential wound dressing material, Int J Biol Macromol, 43, 456, 10.1016/j.ijbiomac.2008.08.010 Zhang, 2009, Rapid adhesion and proliferation of keratinocytes on the gold colloid/chitosan film scaffold, Mater Sci Eng, C29, 908, 10.1016/j.msec.2008.07.040 Zhou, 2008, Electrospun water-soluble carboxyethyl chitosan/poly(vinyl alcohol) nanofibrous membrane as potential wound dressing for skin regeneration, Biomacromolecules, 9, 349, 10.1021/bm7009015