Ronald C. Beavis1, Brain T. Chait1, Henry M. Fales2
1The Rockefeller University, NY 10021, USA
2National Institutes of Health, National Heart, Lung and Blood Institute, Bethsada, Maryland 20892, USA
Tóm tắt
AbstractThe paper reports the discovery of three new matrices for the matrix‐assisted laser desorption of proteins. These new matrices (sinapic, ferulic and caffeic acids) are cinnamic acid derivatives that have several pratical advantages over the nicotinic acid matrices previously used. These materials form much less intense photochemically generated adduct peaks in the protein quasimolecular ion signal and the adduct peaks that are present are easier to resolve. These new matrices are produce intense protonated‐molecule ions from all of the proteins (over 50) so far examined. These new matrices are also very stable in a vacuum, allowing for their convenient use in very high vacuum applications (e.g., Fourier transform ion cyclotron resonance mass spectrometry).
Từ khóa
Tài liệu tham khảo
10.1021/ac00171a028
Hillenkamp F., 1989, Advances in Mass Spectrometry, 354
10.1002/rcm.1290030708
M.Karas A.Ingendoh U.BahrandF.Hillenkamp Biomed. Environm. Mass Spectrom.(1989) submitted.
M.Karas U.Bahr A.IngendohandF.Hillenkamp Angew. Chem.(1989) in press.
