Biomolecular Characteristics of Aspergillus niger Under Cadmium Metal Stress
Tóm tắt
Contamination of heavy metals poses a significant threat to our different ecosystems and their biodegradation as organic contaminants is not possible as heavy metal ions cannot be mineralized to non-toxic forms. Instead, they can be biomobilized into other compounds. Cadmium is supposed to be the most toxic heavy metal occurring naturally in the environment. Fungal biosorption had become an answer to remove toxic metal ions from wastewaters or soils as they can be grown easily and inexpensively. Aspergillus niger is a biomass waste of citric acid production industry and can be used as a biosorbent for this purpose. The cadmium effect on A. niger ITCC 546 and ITCC 6117 has been investigated. The maximum biomass was observed at 475 mM containing medium compared to 500 and 525 mM. The higher concentration of Cd showed more inhibition of fungal cells. The protein synthesis was increased at 475 mM of Cd ions than in control samples. The total RNA expressed from treated fungal cells at 475 mM of Cd ions was in greater quantity than the RNA isolated at 500 mM, while the DNA from mycelia grown at 500 mM were more sheared and degraded than the DNA of 475 mM. The biosorption capability of A. niger ITCC 546 has been found to be much more than that of ITCC 6117.
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