Binary-blend fibber-based capture assay of circulating tumor cells for clinical diagnosis of colorectal cancer
Tóm tắt
In addition to conventional approaches, detecting and characterizing CTCs in patient blood allows for early diagnosis of cancer metastasis. We blended poly(ethylene oxide) (PEO) into nylon-6 through electrospinning to generate a fibrous matbased circulating tumour cells (CTCs) assay. The contents of nylon-6 and PEO in the electrospun blend fibrous mats (EBFMs) were optimized to facilitate high cell-substrate affinity and low leukocyte adsorption. Compared with the IsoFlux System, a commercial instrument for CTC detection, the CTC assay of EBFMs exhibited lower false positive readings and high sensitivity and selectivity with preclinical specimens. Furthermore, we examined the clinical diagnosis accuracy of colorectal cancer, using the CTC assay and compared the results with those identified through pathological analyses of biopsies from colonoscopies. Our positive expressions of colorectal cancer through CTC detection completely matched those recognized through the pathological analyses for the individuals having stage II, III, and IV colorectal cancer. Nevertheless, two in four individuals having stage I colorectal cancer, recognized through pathological analysis of biopsies from colonoscopies, exhibited positive expression of CTCs. Ten individuals were identified through pathological analysis as having no colorectal tumours. Nevertheless, two of these ten individuals exhibited positive expression of CTCs. Thus, in this population, the low cost EBFMs exhibited considerable capture efficiency for the non-invasive diagnosis of colorectal cancer.
Tài liệu tham khảo
Kaiser J. Cancer’s circulation problem. Science. 2010;327:1072–4. https://doi.org/10.1126/science.327.5969.1072.
Nagrath S, et al. Isolation of rare circulating tumour cells in cancer patients by microchip technology. Nature. 2007;450:1235–9. https://doi.org/10.1038/nature06385.
Vona G, et al. Isolation by size of epithelial tumor cells: a new method for the immunomorphological and molecular characterization of circulating tumour cells. Am J Pathol. 2000;156:57–63. https://doi.org/10.1016/S0002-9440(10)64706-2.
Wu T-H, et al. Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter. Lab Chip. 2012;12:1378–83. https://doi.org/10.1039/C2LC21084C.
Chen P, Huang Y-Y, Hoshino K, Zhang X. Multiscale immunomagnetic enrichment of circulating tumor cells: from tubes to microchips. Lab Chip. 2014;14:446–58. https://doi.org/10.1039/C3LC51107C.
Wu C-H, et al. Versatile immunomagnetic nanocarrier platform for capturing cancer cells. ACS Nano. 2013;7:8816–23. https://doi.org/10.1021/nn403281e.
Gascoyne PRC, Noshari J, Anderson TJ, Becker FF. Isolation of rare cells from cell mixtures by dielectrophoresis. Electrophoresis. 2009;30:1388–13984. https://doi.org/10.1002/elps.200800373.
Kavanagh DM, Kersaudy-Kerhoas M, Dhariwal RS, Desmulliez MPY. Current and emerging techniques of fetal cell separation from maternal blood. J Chromatogr B. 2010;878:1905–11. https://doi.org/10.1016/j.jchromb.2010.05.007.
Kang JH, et al. A combined micromagnetic-microfluidic device for rapid capture and culture of rare circulating tumor cells. Lab Chip. 2012;12:2175–81. https://doi.org/10.1039/C2LC40072C.
Ozkumur E, et al. Inertial focusing for tumor antigen-dependent and-independent sorting of rare circulating tumor cells. Sci Transl Med. 2013;5:179ra147. https://doi.org/10.1126/scitranslmed.3005616.
Zhang N, et al. Electrospun TiO2 nanofiber-based cell capture assay for detecting circulating tumor cells from colorectal and gastric cancer patients. Adv Mater. 2012;24:2756–60. https://doi.org/10.1002/adma.201200155.
Yoon HJ, et al. Sensitive capture of circulating tumour cells by functionalized graphene oxide nanosheets. Nat Nanotechnol. 2013;8:735–41. https://doi.org/10.1038/nnano.2013.194.
Hughes AD, et al. Microtube device for selectin-mediated capture of viable circulating tumor cells from blood. Clin Chem. 2012;58:846–53. https://doi.org/10.1373/clinchem.2011.176669.
Dang JM, Leong KW. Myogenic induction of aligned mesenchymal stem cell sheets by culture on thermally responsive electrospun nanofibers. Adv Mater. 2007;19:2775–9. https://doi.org/10.1002/adma.200602159.
Park SY, et al. Carbon nanotube monolayer patterns for directed growth of mesenchymal stem cells. Adv Mater. 2007;19:2530–4. https://doi.org/10.1002/adma.200600875.
Jan E, Kotov NA. Successful differentiation of mouse neural stem cells on layer-by-layer assembled single-walled carbon nanotube composite. Nano Lett. 2007;7:1123–8. https://doi.org/10.1021/nl0620132.
Yang MT, Sniadecki NJ, Chen CS. Geometric considerations of micro- to nanoscale elastomeric post arrays to study cellular traction forces. Adv Mater. 2007;19:3119–23. https://doi.org/10.1002/adma.200701956.
Bucaro MA, Vasquez Y, Hatton BD, Aizenberg J. Fine-tuning the degree of stem cell polarization and alignment on ordered arrays of high-aspect-ratio nanopillars. ACS Nano. 2012;6:6222–30. https://doi.org/10.1021/nn301654e.
Greiner A, Wendorff JH. Electrospinning: a fascinating method for the preparation of ultrathin fibers. Angew Chem Int Ed. 2007;46:5670–703. https://doi.org/10.1002/anie.200604646.
Kao T-H, Chen JK, Cheng CC, Su CI, Chang FC. Low-surface-free-energy polybenzoxazine/polyacrylonitrile fibers for biononfouling membrane. Polymer. 2013;54:258–68. https://doi.org/10.1016/j.polymer.2012.11.020.
Yoshimoto H, Shin YM, Terai H, Vacanti JP. A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. Biomaterials. 2003;24:2077–82. https://doi.org/10.1016/S0142-9612(02)00635-X.
Riboldi SA, et al. Electrospun degradable polyesterurethane membranes: potential scaffolds for skeletal muscle tissue engineering. Biomaterials. 2005;26:4606–15. https://doi.org/10.1016/j.biomaterials.2004.11.035.
Pop-Georgievski O, et al. Nonfouling poly(ethylene oxide) layers end-tethered to polydopamine. Langmuir. 2012;28:14273–83. https://doi.org/10.1021/la3029935.
Chegini N, von Fraunhofer JA, Hay DL, Stone IK, Masterson BJ. The use of nylon pouches to prevent cellular attachment to implanted materials. Biomaterials. 1987;8:315–9. https://doi.org/10.1016/0142-9612(87)90122-0.
Nuhiji E, et al. Biofunctionalization of 3D nylon 6, 6 scaffolds using a two-step surface modification. ACS Appl Mater Interfaces. 2012;4:2912–9. https://doi.org/10.1021/am300087k.
Sterzynska K, Kempisty B, Zawierucha P, Zabel M. Analysis of the specificity and selectivity of anti-EpCAM antibodies in breast cancer cell lines. Folia Histochem Cytobiol. 2012;50:534–41. https://doi.org/10.5603/FHC.2012.0075.
Chen J-K, Zhou G-Y, Huang C-F, Chang J-Y. Two-dimensional periodic relief grating as a versatile platform for selective immunosorbent assay and visualizing of antigens. ACS Appl Mater Interfaces. 2013;5:3348–55. https://doi.org/10.1021/am201632e.
Chen J, Zhou G, Chang C. Real-time multicolor antigen detection with chemoresponsive diffraction gratings of silicon oxide nanopillar arrays. Sens Actuator B. 2013;177:833–40. https://doi.org/10.1016/j.snb.2012.12.019.
Chen J, Zhou G, Huang C, Ko F. Using nanopillars of silicon oxide as a versatile platform for visualizing a selective immunosorbent. Appl Phys Lett. 2013;102:251903. https://doi.org/10.1063/1.4792057.
Alva A, et al. Circulating tumor cells as potential biomarkers in bladder cancer. J Urol. 2015;194:790–8. https://doi.org/10.1016/j.juro.2015.02.2951.
Sánchez-Lorencio MI, et al. Comparison of two types of liquid biopsies in patients with hepatocellular carcinoma awaiting orthotopic liver transplantation. Transplant Proc. 2015;47:2639–42. https://doi.org/10.1016/j.transproceed.2015.10.003.
Joosse SA, Gorges TM, Pantel K. Biology, detection, and clinical implications of circulating tumor cells. EMBO Mol Med. 2014;7:1–11. https://doi.org/10.15252/emmm.201303698.
Schaefgen JR, Trivisonno CF. Polyelectrolyte behavior of polyamides. I. Viscosities of solutions of linear polyamides in formic acid and in sulfuric acid. J Am Chem Soc. 1951;73:4580–5. https://doi.org/10.1021/ja01154a024.
Wang S, et al. Three-dimensional nanostructured substrates toward efficient capture of circulating tumor cells. Angew Chem Int Ed. 2009;48:8970–3. https://doi.org/10.1002/anie.200901668.
Huang MY, et al. Clinical implications and future perspectives of circulating tumor cells and biomarkers in clinical outcomes of colorectal cancer. Transl Oncol. 2016;9:340–7. https://doi.org/10.1016/j.tranon.2016.06.006.
Fong D, et al. Expression of EpCAMMF and EpCAMMT variants in human carcinomas. J Clin Pathol. 2014;67:408–14. https://doi.org/10.1136/jclinpath-2013-201932.