Label-free detection of DNA mutations by SPR: application to the early detection of inherited breast cancer

Springer Science and Business Media LLC - Tập 393 - Trang 1173-1182 - 2008
L. G. Carrascosa1,2, A. Calle3, L. M. Lechuga1,2
1CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Zaragoza, Spain
2Nanobiosensors and Molecular Nanobiophysics Group, Research Center on Nanoscience and Nanotechnology CIN2-(CSIC-ICN), ETSE, Barcelona, Spain
3Microelectronic Institute of Madrid (IMM-CNM-CSIC), Madrid, Spain

Tóm tắt

A screening analysis of DNA hybridization and the presence of DNA mutations using an surface plasmon resonance (SPR) biosensor is shown. The influence of lateral and vertical spacers, as well as several hybridization conditions, was studied to optimize the differentiation between fully complementary and mismatched DNA strands. Our results demonstrated that SPR biosensors were able to detect mismatch sequences related to inherited breast cancer, with high specificity and sensitivity. Using PCR synthetic sequences as targets, mutant sequences were clearly discriminated from fully complementary ones, and detection limits below 50 nM were achieved.

Tài liệu tham khảo

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