Staging of Alzheimer disease-associated neurofibrillary pathology using paraffin sections and immunocytochemistry

Springer Science and Business Media LLC - Tập 112 - Trang 389-404 - 2006
Heiko Braak1, Irina Alafuzoff2, Thomas Arzberger3, Hans Kretzschmar3, Kelly Del Tredici1,4
1Institute for Clinical Neuroanatomy, J.W. Goethe University Clinic, Frankfurt/Main, Germany
2Institute of Clinical Medicine, Pathology, Kuopio University and University Hospital, Kuopio, Finland
3Institute of Neuropathology, Ludwig‐Maximilians‐University, Munich, Germany
4Clinic for Psychiatry and Neurology Winnenden, Winnenden, Germany

Tóm tắt

Assessment of Alzheimer’s disease (AD)-related neurofibrillary pathology requires a procedure that permits a sufficient differentiation between initial, intermediate, and late stages. The gradual deposition of a hyperphosphorylated tau protein within select neuronal types in specific nuclei or areas is central to the disease process. The staging of AD-related neurofibrillary pathology originally described in 1991 was performed on unconventionally thick sections (100 μm) using a modern silver technique and reflected the progress of the disease process based chiefly on the topographic expansion of the lesions. To better meet the demands of routine laboratories this procedure is revised here by adapting tissue selection and processing to the needs of paraffin-embedded sections (5–15 μm) and by introducing a robust immunoreaction (AT8) for hyperphosphorylated tau protein that can be processed on an automated basis. It is anticipated that this revised methodological protocol will enable a more uniform application of the staging procedure.

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