The potential of nanobiopesticide based on zein nanoparticles and neem oil for enhanced control of agricultural pests

Mônica Pascoli1, Felícia Pereira de Albuquerque2, Anderson Kikuchi Calzavara3, Bruno Tinoco-Nunes4, Wanderson Henrique Cruz Oliveira4, Kelly Cristina Gonçalves5, Ricardo Antônio Polanczyk5, Jaqueline Franciosi Della Vechia5, Sidnéia Terezinha Soares de Matos5, Daniel Júnior de Andrade5, Halley Caixeta Oliveira3, Jayme A. Souza-Neto4, Renata de Lima6, Leonardo Fernandes Fraceto1
1Laboratory of Environmental Nanotechnology, Institute of Science and Technology of Sorocaba, São Paulo State University (UNESP), Sorocaba, Brazil
2Institute of Science and Technology of Sorocaba, São Paulo State University (UNESP), Sorocaba, Brazil
3Department of Animal and Plant Biology, Londrina State University, Londrina, Brazil
4Department of Bioprocesses and Biotechnology, Central Multiuser Laboratory, School of Agricultural Sciences, São Paulo State University (UNESP), Botucatu, Brazil
5Department of Crop Protection, College of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, Brazil
6Laboratory of Bioactivity Assessment and Toxicology of Nanomaterials, University of Sorocaba, Sorocaba, Brazil

Tóm tắt

Nanomaterials composed of natural matrices associated with biopesticides have promising applications in sustainable agriculture. In this study, the biopesticide neem oil was encapsulated in zein nanoparticles in order to improve its stability and efficiency. Assays of phytotoxicity (using Phaseolus vulgaris) and biological activity against three pests (Acanthoscelides obtectus, Bemisia tabaci, and Tetranychus urticae) were also performed. The neem oil-loaded zein nanoparticles presented 198 ± 16 nm, polydispersity index of around 0.2, satisfactory physicochemical stability, together with high encapsulation efficiency (> 80%). Pre- and postemergence treatments using this new system did not cause any phytotoxic effects toward P. vulgaris. The neem oil nanobiopesticide exhibited mortality effects on B. tabaci and T. urticae, while the effect against A. obtectus was significantly increased, compared to plain neem oil. The results of the characterization, toxicity, and biological activity studies showed the promising potential of these neem oil-loaded zein nanoparticles for the use in pest management in sustainable agriculture after the required toxicological assessments.

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Tài liệu tham khảo

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