Isolation and culture of tobacco mesophyll protoplasts using a saline medium
Tóm tắt
A new method is proposed for the isolation of tobacco mesophyll protoplasts in which salts were used as the plasmolyticum instead of the traditionally applied sugars. With this method protoplasts were regularly obtained and practically independent of the physiological state of the plants. In addition, a salt medium was developed in which the majority of protoplasts dedifferenciate and divide at a developmental state in which no wall could be detected by plasmolysis. After transferring these cells into another medium they were able to regenerate a wall and to divide.
Tài liệu tham khảo
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