A ribosomal RNA gene intergenic spacer based PCR and DGGE fingerprinting method for the analysis of specific rhizobial communities in soil

Journal of Microbiological Methods - Tập 64 - Trang 366-379 - 2006
Valéria Maia de Oliveira1, Gilson Paulo Manfio1, Heitor Luiz da Costa Coutinho2, Anneke Christina Keijzer-Wolters3, Jan Dirk van Elsas4
1Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas- CPQBA/UNICAMP, CP 6171, CEP 13081-970, Campinas, SP, Brazil
2Embrapa Solos, Rua Jardim Botânico, 1024, CEP 22460-000, Rio de Janeiro, RJ, Brazil
3Plant Research International, P.O. Box 16, 6700AA Wageningen, The Netherlands
4Microbial Ecology Department, University of Groningen, P.O.Box 14, 9750AA, Haren, The Netherlands

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