On the role of Cro in λ prophage induction

Proceedings of the National Academy of Sciences of the United States of America - Tập 102 Số 12 - Trang 4465-4469 - 2005
Sine Lo Svenningsen1, Nina Costantino1, Donald L. Court1, Sankar Adhya1
1National Cancer Institute, Bethesda, MD 20892-4255; and National Cancer Institute, Frederick, MD 21702

Tóm tắt

The lysogenic state of bacteriophage λ is exceptionally stable yet the prophage is readily induced in response to DNA damage. This delicate epigenetic switch is believed to be regulated by two proteins; the lysogenic maintenance promoting protein CI and the early lytic protein Cro. First, we confirm, in the native configuration, the previous observation that the DNA loop mediated by oligomerization of CI bound to two distinct operator regions ( O L and O R ), increases repression of the early lytic promoters and is important for stable maintenance of lysogeny. Second, we show that the presence of the cro gene might be unimportant for the lysogenic to lytic switch during induction of the λ prophage. We revisit the idea that Cro's primary role in induction is instead to mediate weak repression of the early lytic promoters.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 102 Số 12

4465-4469

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