Effect of estradiol‐17β during in vitro growth culture on the growth, maturation, cumulus expansion and development of porcine oocytes from early antral follicles

Animal Science Journal - Tập 86 Số 3 - Trang 251-259 - 2015
Naoko Kubo1, Ilse S. Cayo-Colca1,2, Takashi Miyano1
1Graduate School of Agricultural Science, Kobe University, Kobe, Japan
2Toribio Rodriguez de Mendoza National University, Chachapoyas, Peru

Tóm tắt

AbstractGrowing porcine oocytes from early antral follicles can acquire meiotic and developmental competence under suitable culture conditions, but at lower rates compared to full‐grown oocytes. We postulated that estradiol‐17β (E2) supported the acquisition of meiotic and developmental competence as well as cumulus‐expansion ability during growth culture. Growing oocytes from early antral follicles (1.2 to 1.5 mm in diameter) were grown in vitro for 5 days in a medium containing 0, 10−7, 10−6, 10−5 or 10−4 mol/L E2; after in vitro maturation, 35, 58, 47, 74 and 49% of oocytes matured to metaphase II, 25, 79, 77, 90 and 97% acquired cumulus‐expansion ability, and 23, 54, 63, 89 and 64% were fully surrounded by cumulus cells, respectively. Following maturation, electro‐stimulation was applied to the oocytes grown with 10−5 mol/L E2. After 6 days of culture, in vitro‐grown oocytes developed to the blastocyst stage at a rate similar to that for full‐grown oocytes (31% and 40%, respectively). Therefore, we suggest that the use of E2 during growth culture improves the meiotic and developmental competence of oocytes, cumulus‐expansion ability, and cumulus cell attachment to the oocytes.

Từ khóa


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Thông tin
Thông tin xuất bản

Animal Science Journal

Tập 86 Số 3

251-259

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