Molecular cloning, tissue expression and SNP analysis in the goat nerve growth factor gene
Tóm tắt
In this study, we cloned the full coding region of NGF gene from the caprine ovary. Result showed the caprine NGF cDNA (GenBank Accession No. JQ308184) contained a 726 bp open reading frame encoding a protein with 241 amino acid residues. Bioinformatic analysis indicated that caprine NGF amino acid sequence was 83–99 % identical to that of mouse, pig, dog, human and bovine. It was predicted that caprine NGF contained nine serine phosphorylation loci, four threonine phosphorylation loci and nine specific PKC phosphorylation loci. The NGF mRNA expression pattern showed that NGF gene was expressed highly in ovary. This work provided an important experimental basis for further research on the function of NGF in goat. A single nucleotide polymorphism (A705G) in the coding region of NGF gene was detected by PCR–RFLP and DNA sequencing in 630 goats of three breeds. The frequencies of G allele were 0.52–0.61, and frequencies of A allele were 0.48–0.39 for SN, GZ and BG breeds, respectively. The does with GG genotype had higher litter size than those with GA and AA genotypes (P < 0.05). Hence, the biochemical and physiological functions, together with the results obtained in our investigation, suggest that the NGF gene could serve as a genetic marker for litter size in goat breeding.
Tài liệu tham khảo
Li GA, An XP, Hou JX, Li L, Han D, Yang MM, Wang YN, Zhu GQ, Wang JG, Song YX, Cao BY (2011) Study on polymerization effect of polyembryony genes by SSCP marker and family trees in Chinese goats. Mol Biol Rep 38:739–744
An XP, Wang LX, Hou JX, Li G, Song YX, Wang JA, Yang MM, Cui YH, Cao BY (2011) Novel polymorphisms of goat growth hormone and growth hormone receptor genes and their effects on growth traits. Mol Biol Rep 38:4037–4043
Snider WD (1994) Functions of the neurotrophins during nervous system development: what the knockouts are teaching us. Cell 77:627–638
Garcia-Suarez O, Germana A, Hannestad J, Ciriaco E, Laura R, Naves J, Esteban I, Silos-Santiago I, Vega JA (2000) TrkA is necessary for the normal development of the murine thymus. J Neuroimmunol 108:11–21
Scharfmann R, Tazi A, Polak M, Kanaka C, Czernichow P (1993) Expression of functional nerve growth factor receptors in pancreatic beta-cell lines and fetal rat islets in primary culture. Diabetes 42:1829–1836
Dissen GA, Romero C, Paredes A, Ojeda SR (2002) Neurotrophic control of ovarian development. Microsc Res Tech 59:509–515
Lara HE, Hill DF, Katz KH, Ojeda SR (1990) The gene encoding nerve growth factor is expressed in the immature rat ovary: effect of denervation and hormonal treatment. Endocrinology 126:357–363
Dissen GA, Romero C, Hirshfield AN, Ojeda SR (2001) Nerve growth factor is required for early follicular development in the mammalian ovary. Endocrinology 142:2078–2086
Mayerhofer A, Dissen GA, Parrott JA, Hill DF, Mayerhofer D, Garfield RE, Costa ME, Skinner MK, Ojeda SR (1996) Involvement of nerve growth factor in the ovulatory cascade: trkA receptor activation inhibits gap junctional communication between thecal cells. Endocrinology 137:5662–5670
Raffioni S, Bradshaw RA, Buxser SE (1993) The receptors for nerve growth factor and other neurotrophins. Annu Rev Biochem 62:823–850
Dechant G, Barde YA (1997) Signalling through the neurotrophin receptor p75NTR. Curr Opin Neurobiol 7:413–418
Jana B, Koszykowska M, Czarzasta J (2011) Expression of nerve growth factor and its receptors, trkA and p75, in porcine ovaries. J Reprod Dev 57:468–474
Garcia-Rudaz C, Dorfman M, Nagalla S, Svechnikov K, Soder O, Ojeda SR, Dissen GA (2011) Excessive ovarian production of nerve growth factor elicits granulosa cell apoptosis by setting in motion a tumor necrosis factor alpha/stathmin-mediated death signaling pathway. Reproduction 142:319–331
Ojeda SR, Romero C, Tapia V, Dissen GA (2000) Neurotrophic and cell–cell dependent control of early follicular development. Mol Cell Endocrinol 163:67–71
Romero C, Paredes A, Dissen GA, Ojeda SR (2002) Nerve growth factor induces the expression of functional FSH receptors in newly formed follicles of the rat ovary. Endocrinology 143:1485–1494
Chaves RN, Alves AMCV, Duarte ABG, Araujo VR, Celestino JJH, Matos MHT, Lopes CAP, Campello CC, Name KPO, Bao SN, Figueiredo JR (2010) Nerve growth factor promotes the survival of goat preantral follicles cultured in vitro. Cells Tissues Organs 192:272–282
Weng Q, Shi Z, Kawaguchi M, Watanabe G, Taya K (2008) Expression of nerve growth factor and its receptors trkA and p75 and inhibin alpha-subunit in the ovarian interstitial cells of lactating golden hamsters. J Reprod Dev 54:397–401
Mattioli M, Barboni B, Gioia L, Lucidi P (1999) Nerve growth factor production in sheep antral follicles. Domest Anim Endocrinol 17:361–371
Wu XX, Yang ZP, Shi XK, Li JY, Ji DJ, Mao YJ, Chang LL, Gao HJ (2012) Association of SCD1 and DGAT1 SNPs with the intramuscular fat traits in Chinese Simmental cattle and their distribution in eight Chinese cattle breeds. Mol Biol Rep 39:1065–1071
Alvarez-Castro JM, Carlborg O (2007) A unified model for functional and statistical epistasis and its application in quantitative trait loci analysis. Genetics 176:1151–1167
Combet C, Blanchet C, Geourjon C, Deleage G (2000) NPS@: network protein sequence analysis. Trends Biochem Sci 25:147–150
Salas C, Julio-Pieper M, Valladares M, Pommer R, Vega M, Mastronardi C, Kerr B, Ojeda SR, Lara HE, Romero C (2006) Nerve growth factor-dependent activation of trkA receptors in the human ovary results in synthesis of follicle-stimulating hormone receptors and estrogen secretion. J Clin Endocrinol Metab 91:2396–2403
Carrier A, Rosier MF, Guillemot F, Goguel AF, Pulcini F, Bernheim A, Auffray C, Devignes MD (1996) Integrated physical, genetic, and genic map covering 3 Mb around the human NGF gene (NGFB) at 1p13. Genomics 31:80–89
Elduque C, Laurent P, Hayes H, Rodellar C, Leveziel H, Zaragoza P (1997) Assignment of the beta-nerve growth factor (NGFB) to bovine chromosome 3 band q23 by in situ hybridization. Cytogenet Cell Genet 77:306–307
Chu Q, Zhang Y, Sun D-X, Yu Y, Wang YC, Zhang Y (2010) Identification of the complex vertebral malformation gene in Chinese Holstein and its association with dairy performance traits. Yichuan 32:732–736
Sauna ZE, Kimchi-Sarfaty C (2011) Understanding the contribution of synonymous mutations to human disease. Nat Rev Genet 12:683–691
van der Werf JHJ, Marshall K, Lee S (2007) Methods and experimental designs for detection of QTL in sheep and goats. Small Rumin Res 70:21–31
An XP, Han D, Hou JX, Li G, Wang YN, Li L, Zhu GQ, Wang JG, Song YX, Cao BY (2010) Polymorphism of exon 2 of FSHβ gene and its relationship with reproduction performance in two goat breeds. Agric Sci China 9:880–886
Julio-Pieper M, Lozada P, Tapia V, Vega M, Miranda C, Vantman D, Ojeda SR, Romero C (2009) Nerve growth factor induces vascular endothelial growth factor expression in granulosa cells via a trkA receptor/mitogen-activated protein kinase-extracellularly regulated kinase 2-dependent pathway. J Clin Endocrinol Metab 94:3065–3071
Dissen GA, Hirshfield AN, Malamed S, Ojeda SR (1995) Expression of neurotrophins and their receptors in the mammalian ovary is developmentally regulated: changes at the time of folliculogenesis. Endocrinology 136:4681–4692
Alam M, Pravica V, Fryer AA, Hawkins CP, Hutchinson IV (2005) Novel polymorphism in the promoter region of the human nerve growth-factor gene. Int J Immunogenet 32:379–382