Heterologous Expression and Genetic Engineering of the Tubulysin Biosynthetic Gene Cluster Using Red/ET Recombineering and Inactivation Mutagenesis

Chemistry & Biology - Tập 19 Số 3 - Trang 361-371 - 2012
Yi Chai1, Shiping Shan2,1, Kira J. Weissman1, Shengbiao Hu2,1, Youming Zhang3, Rolf Müller1
1Helmholtz Institute for Pharmaceutical Research Saarland, Helmholtz Centre for Infection Research, Department of Pharmaceutical Biotechnology, Saarland University, Saarbrücken 66041, Germany
2College of Life Science, Key Laboratory of Microbial Molecular Biology of Hunan Province, Hunan Normal University, Changsha 410081, Hunan Province, People's Republic of China
3Research & Development, Gene Bridges GmbH, Saarland University, Saarbrücken 66041, Germany

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