Refinement of γ δ resolvase reveals a strikingly flexible molecule

Hatfull, 1988, Resolvases and DNA-invertases: a family of enzymes active in site-specific recombination, 357 Sherratt, 1989, Tn3 and related transposable elements: site-specific recombination and transposition, 163 Hatfull, 1988, Site-specific recombination by the γ δ resolvase, Symp. Soc. Gen. Microbiol, 43, 149 Grindley, 1985, Transpositional recombination in prokaryotes, Annu. Rev. Biochem, 54, 863, 10.1146/annurev.bi.54.070185.004243 Grindley, 1982, Transposon-mediated site- specific recombination: identification of three binding sites for resolvase at the res sites of γ δ and Tn3, Cell, 30, 19, 10.1016/0092-8674(82)90007-1 Kitts, 1983, Transposon-encoded site-specific recombination: nature of the Tn3 sequences which constitute the recombination site res, EMBO J, 329, 346 Salvo, 1987 Salvo, 1988, The δ resolvase bends the res site into a recombinogenic complex, EMBO J, 7, 3609, 10.1002/j.1460-2075.1988.tb03239.x Hatfull, 1987, The γ δ resolvase induces an unusual DNA structure at the recombinational crossover point, Cell, 49, 103, 10.1016/0092-8674(87)90760-4 Reed, 1981, Transposon- mediated site-specific recombination in vitro: DNA cleavage and protein–DNA linkage at the recombination site, Cell, 25, 721, 10.1016/0092-8674(81)90179-3 Wells, 1984, Analysis of the γ δ res sites required for site-specific recombination and gene expression, J. Mol. Biol, 179, 667, 10.1016/0022-2836(84)90161-X Reed, 1981, Transposon-mediated site-specific recombination: a defined in vitro system, Cell, 25, 713, 10.1016/0092-8674(81)90178-1 Reed, 1984, Resolvase-mediated recombination intermediates contain a serine residue covalently linked to DNA, Cold Spring Harbor Symp. Quant. Biol, 49, 245, 10.1101/SQB.1984.049.01.028 Hatfull, 1986, Analysis of γ δ resolvase mutants in vitro : evidence for an interaction between serine-10 of resolvase and site I of res, Proc. Natl. Acad. Sci. U S A, 83, 5429, 10.1073/pnas.83.15.5429 Krasnow, 1983, Site-specific relaxation and recombination by the Tn3 resolvase: recognition of the DNA path between oriented res sites, Cell, 32, 1313, 10.1016/0092-8674(83)90312-4 Abdel-Meguid, 1984, Cleavage of the site-specific recombination protein γ δ resolvase: the smaller of two fragments binds DNA specifically, Proc . Natl . Acad . Sci. U S A, 81, 2001, 10.1073/pnas.81.7.2001 Hatfull, 1989, Preparation of heavy-atom derivatives using site-directed mutagenesis, J. Mol. Biol, 208, 661, 10.1016/0022-2836(89)90156-3 Sanderson, 1990, The crystal structure of the catalytic domain of the site-specific recombination enzyme γ δ resolvase at 2.7 å resolution, Cell, 63, 1323, 10.1016/0092-8674(90)90427-G Hughes, 1990, Cooperativity mutants of the γ δ resolvase identify an essential interdimer interaction, Cell, 63, 1331, 10.1016/0092-8674(90)90428-H Hughes, 1993, Protein interactions directing resolvase site- specific recombination: a structure–function analysis, EMBO J, 12, 1447, 10.1002/j.1460-2075.1993.tb05788.x Luzatti, 1952, Acta Crystallogr, 5, 802, 10.1107/S0365110X52002161 Brändén, 1991 Weber, 1982, Crystallization of resolvase, a repressor that also catalyzes site-specific DNA recombination, J. Mol. Biol, 157, 689, 10.1016/0022-2836(82)90508-3 Abdel-Meguid, 1986, Preliminary X-ray diffraction studies of the putative catalytic domain of γ δ resolvase from Escherichia coli, J. Biol. Chem, 261, 15934, 10.1016/S0021-9258(18)66655-4 Rice, 1994, Model for a DNA mediated synaptic complex suggested by crystal packing of γ δ resolvase subunits, EMBO J, 13, 1514, 10.1002/j.1460-2075.1994.tb06413.x Richardson, 1981, The anatomy and taxonomy of protein structure, Adv. Protein Chem, 34, 167, 10.1016/S0065-3233(08)60520-3 Salemme, 1983, Structural properties of protein β -sheets, Prog. Biophys. Molec. Biol, 42, 95, 10.1016/0079-6107(83)90005-6 Dobson, 1990, Hinge-bending and folding, Nature, 348, 198, 10.1038/348198a0 Steitz, 1983, Ligand induced conformational changes in proteins, Ciba Found. Symp, 93, 25 Richards, 1985, Calculation of molecular volumes and areas for structures of known geometry, Methods Enzymol, 115, 440, 10.1016/0076-6879(85)15032-9 Connolly, 1983, Solvent-accessible surfaces of proteins and nucleic acids, Science, 221, 709, 10.1126/science.6879170 Rashin, 1986, Internal cavities and buried waters in globular proteins, Biochemistry, 25, 3619, 10.1021/bi00360a021 Janin, 1988, Surface, subunit interfaces and interior of oligomeric proteins, J. Mol.Biol, 204, 155, 10.1016/0022-2836(88)90606-7 Chothia, 1975, Principles of protein– protein recognition, Nature, 256, 705, 10.1038/256705a0 Klippel, 1988, Isolation and characterization of unusual gin mutants, EMBO J, 7, 3983, 10.1002/j.1460-2075.1988.tb03286.x Haffter, 1988, Enhancer-independent mutants of the Cin recombinase have a relaxed topological specificity, EMBO J, 7, 391, 10.1002/j.1460-2075.1988.tb03287.x Klippel, 1993, Analysis of strand exchange and DNA binding of enhancer- independent Gin recombinase mutants, EMBO J, 12, 1047, 10.1002/j.1460-2075.1993.tb05746.x Wang, 1985, Resolution of phase ambiguity in macromolecular crystallography, Methods Enzymol, 115, 90, 10.1016/0076-6879(85)15009-3 Bricogne, 1974, Geometric sources of redundancy in intensity data and their use for phase determination. Acta, Crystallogr. A, 30, 395, 10.1107/S0567739474010722 Bricogne, 1976, Methods and programs for direct- space exploitation of geometric redundancies, Acta Crystallogr. A, 32, 832, 10.1107/S0567739476001691 Fletterick, 1976, The combination of independent phase information obtained from separate protein structure determinations of yeast hexokinase, Acta Crystallogr. A, 32, 125, 10.1107/S0567739476000235 Hamlin, 1985, Multiwire area X-ray diffractometers, Methods Enzymol, 114, 416, 10.1016/0076-6879(85)14029-2 Anderson, 1986 Hendrickson, 1985, Stereochemically restrained refinement of macromolecular structures, Methods Enzymol, 115, 252, 10.1016/0076-6879(85)15021-4 Brünger, 1987, Crystallographic R factor refinement by molecular dynamics, Science, 235, 458, 10.1126/science.235.4787.458 Brünger, 1990 Jones, 1987, A graphics model building and refinement system for macromolecules, J. Appl. Crystallogr, 11, 1 Engh, 1991, Accurate bond and angle parameters for X-ray structure refinement, Acta Crystallogr. A, 47, 392, 10.1107/S0108767391001071 Lee, 1971, The interpretation of protein structures: estimation of static accessibility, J. Mol. Biol, 55, 379, 10.1016/0022-2836(71)90324-X Priestle, 1988, RIBBON: a stereo cartoon drawing program for proteins, J. Appl. Crystallogr, 21, 572, 10.1107/S0021889888005746