Mechanism of enzymatic dehairing of skins using a bacterial alkaline protease

Alexander, 1986, Influence of proteoglycan removal on opening up in the beamhouse, J. Am. Leather Chem. Assoc., 81, 85 Anderson, 1965, The O-serine linkage in peptides of chondroitin 4 or 6 sulfate, J. Biol. Chem., 240, 156, 10.1016/S0021-9258(18)97630-1 Bancroft, 2004, Theory and practice of histological techniques, 139 Bitter, 1962, A modified uronic acid carbazole reaction, Anal. Biochem., 4, 330, 10.1016/0003-2697(62)90095-7 Bjornsson, 1993, Simultaneous preparation and quantitation of proteoglycans by precipitation with alcian blue, Anal. Biochem., 210, 282, 10.1006/abio.1993.1197 Blaschke, 1996, Distinct isoforms of chicken decorin contain either one or two dermatan sulfate chains, J. Biol. Chem., 271, 30347, 10.1074/jbc.271.48.30347 Cahan, 2001, A secreted aminopeptidase of Pseudomonas aeruginosa. Identification, primary structure and relationship to other aminopeptidases, J. Biol. Chem., 276, 43645, 10.1074/jbc.M106950200 Cantera, 2001, Hair save unhairing process. Part 3. Cementing substances and the basement membrane, J. Soc. Leath. Technol. Chem., 85, 93 Cantera, 2001, Hair saving unhairing process, Part 1. Epidermis and the characteristics of bovine hair, J. Soc. Leath. Technol. Chem., 85, 1 Carrino, 2003, Age related changes in the proteoglycans of human skins, J. Biol. Chem., 278, 17566, 10.1074/jbc.M300124200 Chopra, 1985, Dermatan sulphate is located on serine-4 of bovine skin proteodermatan sulphate, Biochem. J., 15, 277, 10.1042/bj2320277 El Baba, 2000, The effects of hair saving on unhairing reactions, J. Soc. Leath. Technol. Chem., 84, 48 Elson, 1933, A colorimetric method for the determination of glucosamine and chondrosamine, Biochem. J., 27, 1824, 10.1042/bj0271824 Hardingham, 1983, Molecular conformations in proteoglycan aggregation, Biochem. Soc. Trans., 11, 128, 10.1042/bst0110128 Hedbom, 1993, Binding of fibromodulin and decorin to separate sites on fibrillar collagens, J. Biol. Chem., 268, 27307, 10.1016/S0021-9258(19)74250-1 Heidemann, 1982, Newer developments in the chemistry and structure of collagenous connective tissues and their impact on leather manufacture, J. Soc. Leath. Technol. Chem., 66, 21 Krusius, 1986, Primary structure of an extracellular matrix proteoglycan core protein deduced from cloned cDNA, Proc. Natl. Acad. Sci. USA, 83, 7683, 10.1073/pnas.83.20.7683 Kunitz, 1947, Crystalline soyabean trypsin inhibitor II. General properties, J. Gen. Physiol., 30, 291, 10.1085/jgp.30.4.291 Menderes, 2000, The mechanism and effects of collagen amide group hydrolysis during liming, J. Soc. Leath. Technol. Chem., 83, 107 Mozersky, 2003, Estimation of the sulfated glycosaminoglycan content of bovine skin with alcian blue, J. Am. Leather Chem. Assoc., 98, 337 Neuman, 1950, The determination of hydroxyproline, J. Biol. Chem., 184, 299, 10.1016/S0021-9258(19)51149-8 Neuman, 1950, The determination of collagen and elastin in tissues, J. Biol. Chem., 186, 549, 10.1016/S0021-9258(18)56248-7 Obrink, 1972, Isolation and partial characterization of a dermatan sulphate proteoglycan from pig skin, Biochim. Biophys. Acta, 264, 354, 10.1016/0304-4165(72)90300-5 Official Methods of Analysis, Sec. 1, 1965. Society of Leather Trades’ Chemists. O’Flaherty, 1978, vol. 1 Pearson, 1983, The NH2-terminal amino acid sequence of bovine skin proteodermatan sulfate, J. Biol. Chem., 258, 15101, 10.1016/S0021-9258(17)43777-X Puvanakrishnan, 1988, Enzyme technology in beamhouse practice, 92 Ramasami, 1999, Beamhouse and tanning operations: process chemistry revisited, J. Soc. Leath. Technol. Chem., 83, 39 Schonherr, 1995, Interaction of biglycan with Type I Collagen, J. Biol. Chem., 270, 2776, 10.1074/jbc.270.6.2776 Schonherr, 1995, Decorin-type I collagen interaction. Presence of separate core protein domains, J. Biol. Chem., 270, 8877, 10.1074/jbc.270.15.8877 Scott, 1988, Proteoglycan – fibrillar collagen interaction, Biochem. J., 252, 313, 10.1042/bj2520313 Scott, 2001, Structure and function in extracellular matrices depend on interactions between anionic glycosaminoglycans, Pathol. Biol., 49, 284, 10.1016/S0369-8114(01)00152-3 Stanley, 1982, Structure and function of basement membrane, J. Invest. Dermatol., 79, 69, 10.1111/1523-1747.ep12545830 Svensson, 1995, Decorin-binding sites for collagen type I are mainly located in leucine-rich repeats 4-5, J. Biol. Chem., 270, 20712, 10.1074/jbc.270.35.20712 Vesentini, 2005, Estimation of the binding force of the collagen molecule-decorin core protein complex in collagen fibril, J. Biomech., 38, 433, 10.1016/j.jbiomech.2004.04.032 Wart, 1981, A continuous spectrophotometric assay for Clostridium histolyticum collagenase, Anal. Biochem., 113, 356, 10.1016/0003-2697(81)90089-0 Yates, 1972, Studies in depilation. Part X. The mechanism of the enzyme depilation process., J. Soc. Leath. Technol. Chem., 56, 158