Optimization of growth determinants of a potent cellulolytic bacterium isolated from lignocellulosic biomass for enhancing biogas production

Springer Science and Business Media LLC - Tập 18 - Trang 1565-1583 - 2016
Ramansu Goswami1, Suprabhat Mukherjee2, Amit Kumar Chakraborty1, Srinivasan Balachandran1, Santi P. Sinha Babu2, Shibani Chaudhury1
1Department of Environmental Studies, Visva-Bharati University, Santiniketan, India
2Department of Zoology, Visva-Bharati University, Santiniketan, India

Tóm tắt

Anaerobic digestion of lignocellulosic biomass is limited by inefficient hydrolysis of recalcitrant substrates, leading to low biogas yields where bacteria can be utilized successfully. In this study, we have chosen ten cellulose-degrading bacteria from active anaerobic slurry identified as Enterobacter ludwigii, Klebsiella pneumoniae, Pantoea agglomerans, Bacillus subtilis, Bacillus pumilus, Bacillus anthracis, Pseudomonas sp., Enterobacteriaceae bacterium Staphylococcus warneri, and Bacillus safensis; among them, E. ludwigii was found to be the most potent having an endoglucanase gene in the genome. The growth conditions of E. ludwigii were further optimized using Response Surface Methodology that designated 35 °C temperature, 6.5 pH, 5 % carboxymethylcellulose, 5 % yeast extract, 1 % ammonium nitrate as optimum growth conditions. The optimized growth module found to accelerate cellulase production at both transcription and translation level that in turn enhanced biogas production inside anaerobic digester as well. Finally, the growth-cellulase production relationship could be helpful in efficient industrial applications.

Tài liệu tham khảo

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