In Vitro and In Vivo Antitumor Activity of Scutellaria barbate Extract on Murine Liver Cancer

Springer Science and Business Media LLC - Tập 16 Số 6 - Trang 4389-4400
Zhijun Dai1, Jie Gao1, Zong-Fang Li1, Ji Zongzheng2, Huafeng Kang1, Haitao Guan1, Yan Diao1, Baofeng Wang1, Xi-Jing Wang1
1Department of Oncology, The Second Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710004, China
2Department of General Surgery, the Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004, China

Tóm tắt

In the present study, we investigated the in vitro and in vivo antitumor effects of crude extract of Scutellaria barbate (CE-SB) on mouse hepatoma H22 cells. The MTT assay was used to determine the growth inhibition of H22 cells in vitro. The in vivo therapeutic effects of CE-SB were determined using H22 tumor bearing mice. Besides, the body weight, tumor weight, thymus index and spleen index of H22 bearing mice were also measured. The tumor inhibitory rate (IR) was calculated according to the mean weight of tumor (MWT). The phagocytotic function of macrophages was examined by observing peritoneal macrophages phagocytize chicken RBC. The results showed that CE-SB could inhibit the growth of hepatoma H22 Cells in vitro and in vivo. Furthermore, CE-SB could improve immune function of H22 tumor bearing mice. Together these results indicate that CE-SB has antitumor activity and seems to be safe and effective for the use of anti-tumor therapy.

Từ khóa


Tài liệu tham khảo

Jemal, 2011, Global cancer statistics, CA Cancer J. Clin., 61, 69, 10.3322/caac.20107

Dai, 2007, Enhanced therapeutic effects of combined chemotherapeutic drugs and midkine antisense oligonucleotides for hepatocellular carcinoma, World J. Gastroenterol., 13, 1989, 10.3748/wjg.v13.i13.1989

Yu, 2007, Antitumor Activity of Chloroform Fraction of Scutellaria barbata and its Active Constituents, Phytother. Res., 21, 817, 10.1002/ptr.2062

Vickers, 2002, Botanical medicines for the treatment of cancer: Rationale, overview of current data, and methodological considerations for phase I and II trials, Cancer Invest., 20, 1069, 10.1081/CNV-120005926

Lee, 2004, Inhibitory effects of Scutellaria barbata D.Don on human uterine leiomyomal smooth muscle cell proliferation through cell cycle analysis, Int. Immunopharmacol., 4, 447, 10.1016/j.intimp.2003.12.012

Lin, 1996, The anti-inflammatory activity of Scutellaria rivularis extracts and its active components, baicalin, baicaleinand wogonin, Am. J. Chin. Med., 24, 31, 10.1142/S0192415X96000050

Lee, 2004, Differential Inhibition of Scutellaria barbata D. Don (Lamiaceae) on HCG-Promoted Proliferation of Cultured Uterine Leiomyomal and Myometrial Smooth Muscle Cells, Immunopharmacol. Immunotoxicol., 26, 329, 10.1081/IPH-200026841

Goh, 2005, Inhibitory effects of a chemically standardized extract from Scutellaria barbata in human colon cancer cell lines, LoVo, J. Agric. Food Chem., 53, 8197, 10.1021/jf051506+

Yin, 2004, Anticanceractivity and mechanism of Scutellaria barbata extract on human lung cancer cell line A549, Life Sci., 75, 2233, 10.1016/j.lfs.2004.05.015

Cha, 2004, Methylene chloride fraction of Scutellaria barbata induces apoptosis in human U937 leukemia cells via the mitochondrial signaling pathway, Clin. Chim. Acta, 348, 41, 10.1016/j.cccn.2004.04.013

Suh, 2007, Chemoprevention of Scutellaria bardata on Human Cancer Cells and Tumorigenesis in Skin Cancer, Phytother. Res., 21, 135, 10.1002/ptr.2010

Dai, 2008, Antiproliferative and apoptosis-inducing activity of Scutellaria barbate containing serum on mouse hepatoma H22 cells, Zhong Yao Cai, 31, 550

Lin, 2006, Inhibition activity of Scutellariae barbata extracts against human hepatocellular carcinoma cells, Nan Fang Yi Ke Da Xue Xue Bao, 26, 591

Lee, 2004, Scutellaria barbata D. Don induces c-fos gene expression in human uterine leiomyomal cells by activating beta2-adrenergic receptors, Int. J. Gynecol. Cancer, 14, 526, 10.1111/j.1048-891x.2004.014315.x

Wang, 2004, Studies on the flavonoids in herb from Scutellaria barbata, Zhong Guo Zhong Yao Za Zhi, 29, 957

Dai, 2007, Bioactive ent-clerodane diterpenoids from Scutellaria barbata, Planta Med., 73, 1217, 10.1055/s-2007-990215

Qu, 2010, Two new cytotoxic ent-clerodane diterpenoids from Scutellaria barbata, J. Asian Nat. Prod. Res., 12, 859, 10.1080/10286020.2010.507546

Dai, 2011, New norditerpenoid alkaloids from Scutellaria barbata with cytotoxic activities, Nat. Prod. Res., 15, 1

Mi, 2010, Simultaneous determination of 7 active ingredients in Scutellaria barbata D. Don by capillary micellar electrokinetic chromatography, Se Pu, 28, 209

Yu, 2004, Chemical composition and antimicrobial activity of the essential oil of Scutellaria barbata, Phytochemistry, 65, 881, 10.1016/j.phytochem.2004.02.005

Tashiro, 1988, "Serum pharmacology" and "serum pharmacochemistry"-New worlds of drug level monitoring starting from the pharmacology of Kampo remedies, TDM Res., 5, 54

Miura, 2007, Effect of Apple Polyphenol Extract on Hepatoma Proliferation and Invasion in Culture and on Tumor Growth, Metastasis, and Abnormal Lipoprotein Profiles in Hepatoma-Bearing Rats, Biosci. Biotechnol. Biochem., 71, 2743, 10.1271/bbb.70359

Nishida, 2003, Mechanisms for the vasodilations induced by Ginkgo biloba extract and its main constituent, bilobalide, in rat aorta, Life Sci., 72, 2659, 10.1016/S0024-3205(03)00177-2

Er, 2007, Anti-proliferative and mutagenic activities of aqueous and methanol extracts of leaves from Pereskia bleo (Kunth) DC (Cactaceae), J. Ethnopharmacol., 113, 448, 10.1016/j.jep.2007.06.026

Wang, 2010, Preparative separation of four individual flavonoids in Scutellaria barbata D. Don based on high selectivity polymeric adsorbents with different polarities, J. Chromatogr. B, 878, 3375, 10.1016/j.jchromb.2010.10.023

Marconett, 2010, BZL101, a phytochemical extract from the Scutellaria barbata plant, disrupts proliferation of human breast and prostate cancer cells through distinct mechanisms dependent on the cancer cell phenotype, Cancer Biol. Ther, 10, 397, 10.4161/cbt.10.4.12424

Wu, 2004, Effect of resveratrol and in combination with 5-FU on murine liver cancer, World J. Gastroenterol., 10, 3048, 10.3748/wjg.v10.i20.3048

Song, 2010, Experiments on efficiency enhancing and toxicity reducing of scutellaria barbata polysaccharides on cytoxan and its immunology mechanism, Acta Chin. Med. Pharm., 28, 48

Dai, 2008, Scutellaria barbate extract induces apoptosis of hepatoma H22 cells via the mitochondrial pathway involving caspase-3, World J. Gastroenterol., 14, 7321, 10.3748/wjg.14.7321

Blattner, 2001, Screening assays for the mitochondrial permeability transition using a fluorescence multiwell plate reader, Anal. Biochem., 295, 220, 10.1006/abio.2001.5219

Daiki, 2007, Effect of Apple Polyphenol Extract on Hepatoma Proliferation and Invasion in Culture and on Tumor Growth, Metastasis, and Abnormal Lipoprotein Profiles in Hepatoma-Bearing Rats, Biosci. Biotechnol. Biochem, 71, 2743, 10.1271/bbb.70359

Dongre, 2007, Antitumor Activity of the Methanol Extract of Hypericum hookerianum Stem Against Ehrlich Ascites Carcinoma in Swiss Albino Mice, J. Pharmacol. Sci., 103, 354, 10.1254/jphs.FP0061088

Liu, 2008, Antitumor effect and mechanism of Gecko on human esophageal carcinoma cell lines in vitro and xenografted sarcoma 180 in Kunming mice, World J. Gastroenterol., 14, 3990, 10.3748/wjg.14.3990