Exploring the mode of action of ebselen in Trypanosoma brucei hexokinase inhibition

April C. Joice1, Michael T. Harris1, Elizabeth W. Kahney1, Heidi C. Dodson1, Andrew G. Maselli2, Daniel C. Whitehead3, James C. Morris1
1Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, United States
2Department of Biological Sciences, Chicago State University, Chicago, IL 60628, United States
3Department of Chemistry, Clemson University, Clemson, SC 29634, United States

Tài liệu tham khảo

Bayer, 1985, 3-(N-Maleimido-propionyl)biocytin: a versatile thiol-specific biotinylating reagent, Anal. Biochem., 149, 529, 10.1016/0003-2697(85)90609-8 Borges, 2005, Effect of diphenyl diselenide, diphenyl ditelluride and ebselen on cerebral Na(+), K(+)-ATPase activity in rats, Toxicology, 215, 191, 10.1016/j.tox.2005.07.002 Brun, 2010, Human African trypanosomiasis, Lancet, 375, 148, 10.1016/S0140-6736(09)60829-1 Chambers, 2008, The anti-trypanosomal agent lonidamine inhibits Trypanosoma brucei hexokinase 1, Mol. Biochem. Parasitol., 158, 202, 10.1016/j.molbiopara.2007.12.013 Chambers, 2008, Assembly of heterohexameric trypanosome hexokinases reveals that hexokinase 2 is a regulable enzyme, J. Biol. Chem., 283, 14963, 10.1074/jbc.M802124200 Chambers, 2008, Residues in an ATP binding domain influence sugar binding in a trypanosome hexokinase, Biochem. Biophys. Res. Commun., 365, 420, 10.1016/j.bbrc.2007.10.192 Dodson, 2010, Quercetin, a fluorescent bioflavanoid, inhibits Trypanosoma brucei hexokinase 1, Exp. Parasitol., 127, 423, 10.1016/j.exppara.2010.10.011 Harris, 2013, Interrogating a hexokinase-selected small molecule library for inhibitors of Plasmodium falciparum hexokinase, Antimicrob. Agents Chemother., 57, 3717, 10.1128/AAC.00662-13 Lu, 2013, Inhibition of bacterial thioredoxin reductase: an antibiotic mechanism targeting bacteria lacking glutathione, FASEB J., 27, 1394, 10.1096/fj.12-223305 Lu, J., Vodnala, S.K., Gustavsson, A.L., Gustafsson, T.N., Sjoberg, B., Johansson, H.A., Kumar, S., Tjernberg, A., Engman, L., Rottenberg, M.E., Holmgren, A., in press. Ebsulfur is a benzisothiazolone cytocidal inhibitor targeting the trypanothione reductase of Trypanosoma brucei. J. Biol. Chem. http://dx.doi.org/jbc.M113.495101 Misset, 1984, Simultaneous purification of hexokinase, class-I fructose-bisphosphate aldolase, triosephosphate isomerase and phosphoglycerate kinase from Trypanosoma brucei, Eur. J. Biochem., 144, 475, 10.1111/j.1432-1033.1984.tb08490.x Misset, 1986, Glycolytic enzymes of Trypanosoma brucei. Simultaneous purification, intraglycosomal concentrations and physical properties, Eur. J. Biochem., 157, 441, 10.1111/j.1432-1033.1986.tb09687.x Morris, 2006, Activity of a second Trypanosoma brucei hexokinase is controlled by an 18-amino-acid C-terminal tail, Eukaryot. Cell, 5, 2014, 10.1128/EC.00146-06 Sakurai, 2006, Ebselen, a seleno-organic antioxidant, as an electrophile, Chem. Res. Toxicol., 19, 1196, 10.1021/tx0601105 Schewe, 1995, Molecular actions of ebselen – an antiinflammatory antioxidant, Gen. Pharmacol., 26, 1153, 10.1016/0306-3623(95)00003-J Sharlow, E., Golden, J.E., Dodson, H., Morris, M., Hesser, M., Lyda, T., Leimgruber, S., Schroeder, C.E., Flaherty, D.P., Weiner, W.S., Simpson, D., Lazo, J.S., Aube, J., Morris, J.C., 2010a. Identification of Inhibitors of Trypanosoma brucei Hexokinases, Probe Reports from the NIH Molecular Libraries Program, Bethesda (MD). Sharlow, 2010, A target-based high throughput screen yields Trypanosoma brucei hexokinase small molecule inhibitors with antiparasitic activity, PLoS Negl. Trop. Dis., 4, e659, 10.1371/journal.pntd.0000659 Terentis, 2010, The selenazal drug ebselen potently inhibits indoleamine 2,3-dioxygenase by targeting enzyme cysteine residues, Biochemistry, 49, 591, 10.1021/bi901546e Yamaguchi, 1998, Ebselen in acute ischemic stroke: a placebo-controlled, double-blind clinical trial. Ebselen Study Group, Stroke, 29, 12, 10.1161/01.STR.29.1.12