Localization of 70-kDa stress protein induction in gerbil brain after ischemia
Tóm tắt
Induction of the 70-kDa heat shock protein, hsp70, has been demonstrated in brain following experimental stroke. In the present study, hsp70 was localized in gerbil brain at intervals after transient ischemia using a monoclonal antibody specific for stress-inducible forms of hsp70-related proteins. Induced immunoreactivity was found only in neurons, primarily in hippocampus, striatum, entorhinal cortex and some neocortical regions. Notably hsp70 accumulation was minimal in hippocampal CA1 neurons which die after brief ischemic episodes, but was most pronounced in dentate granule cells and CA3 neurons which are spared. The peak of CA3 immunoreactivity occurred at 48-h recirculation, at the onset of CA1 neuron loss at 2–4 days, demonstrating that hsp70 induction is also a component of this delayed hippocampal pathophysiology rather than a direct response to the metabolic disruption of the initial ischemic episode. These results suggest that hsp70 immunocytochemistry may serve as a marker for neuronal circuitry involved in proposed excitotoxic mechanisms after ischemia and other stresses. Control animals showed immunoreactivity in ependymal cells lining the ventricles, indicating a role for hsp70 in normal functioning of these specialized cells.
Tài liệu tham khảo
Benveniste H, Drejer J, Schousboe A, Diemer NH (1984) Elevation of the extracellular concentrations of glutamate and aspartate in rat hippocampus during transient cerebral ischemia monitored by intracerebral microdialysis. J Neurochem 43:1369–1374
deBlas AL, Cherwinski HM (1983) Detection of antigens on nitrocellulose paper immunoblots with monoclonal antibodies. Anal Biochem 133:214–219
Bond U, Schlesinger MJ (1985) Ubiquitin is a heat shock protein in chicken embryo fibroblasts. Mol Cell Biol 5: 949–956
Bond U, Schlesinger MJ (1986) The chicken ubiquitin gene contains a heat shock promoter and expresses an unstable mRNA in heat shocked cells. Mol Cell Biol 6:4602–4610
Brierley JB, Graham DI (1984) Hypoxia and vascular disorders of the central nervous system. In: Adams JH, Corsellis JAN, Duchen IW (eds) Greenfield's neuropathology. John Wiley & Sons, New York, pp 125–207
Brightman MW, Palay SL (1963) The fine structure of ependyma in the brain of the rat. J Cell Biol 19:415–439
Chappell TG, Welch WJ, Schlossman DM, Palter KB, Schlesinger MJ, Rothman JE (1986) Uncoating ATPase is a member of the 70 kDa family of stress proteins. Cell 45: 3–13
Collins RC (1986) Selective vulnerability of brain: new insights from the excitatory synapse. Metab Brain Dis 1:231–240
Diemer NH, Siemkovicz E (1980) Increased 2-deoxyglucose uptake in hippocampus, globus pallidus and substantia nigra after cerebral ischemia. Acta Neurol Scand 61:56–63
Dienel GA, Cruz NF, Rosenfeld SJ (1985) Temporal profiles of proteins responsive to transient ischemia. J Neurochem 44:600–610
Dienel GA, Kiessling M, Jacewicz M, Pulsinelli WA (1986) Synthesis of heat shock proteins in rat brain cortex after transient ischemia. J Cereb Blood Flow Metab 6:505–510
Dragunow M, Robertson HA (1987) Kindling stimulation induces c-fos protein(s) in granule cells of rat dentate gyrus. Nature 329:441–442
Dragunow M, Robertson HA (1987) Generalized seizures induce c-fos protein(s) in mammalian neurons. Neurosci Lett 82:157–161
Fried RL, Nowak TS Jr (1987) Opioid peptide levels in gerbil brain after transient ischemia: lasting depletion of hippocampal dynorphin. Stroke 18:765–770
Hunt SP, Pini A, Evan G (1987) Induction of c-fos-like protein in spinal cord neurons following sensory stimulation. Nature 328:632–634
Jacewicz M, Kiessling M, Pulsinelli WA (1986) Selective gene expression in focal cerebral ischemia. J Cereb Blood Flow Metab 6:263–272
Johansen FF, Jorgensen MB, Diemer NH (1986) Ischemic CA-1 pyramidal cell loss is prevented by preischemic colchicine destruction of dentate granule cells. Brain Res 377: 344–347
Johansen FF, Zimmer J, Diemer NH (1987) Early loss of somatostatin neurons in dentate hilus after cerebral ischemia in the rat precedes CA-1 pyramidal cell loss. Acta Neuropathol (Berl) 73:110–114
Jorgensen MB, Johansen FF, Diemer NH (1987) Removal of the entorhinal cortex protects hippocampal CA-1 neurons from ischemic damage. Acta Neuropathol (Berl) 73:189–194
Kiessling M, Dienel GA, Jacewicz M, Pulsinelli WA (1986) Protein synthesis in postischemic rat brain: a two-dimensional electrophoretic analysis. J Cereb Blood Flow Metab 6:642–649
Kirino T (1982) Delayed neuronal death in the gerbil hippocampus following ischemia. Brain Res 239:57–69
Kirino T, Sano K (1984) Fine structure of delayed neuronal death following ischemia in the gerbil hippocampus. Acta Neuropathol (Berl) 62:209–218
Lim L, Hall C, Leung T, Whatley SA (1984) The relationship of the rat brain 68-kDa microtubule-associated protein with synaptosomal plasma membranes and with theDrosophila 70-kDa heat shock protein. Biochem J 224:677–680
Linden T, Kalimo H, Wieloch T (1987) Protective effect of lesion to the glutamatergic cortico-striatal projections on the hypoglycemic nerve cell injury in rat striatum. Acta Neuropathol (Berl) 74:335–344
Lowe DG, Moran LA (1986) Molecular cloning and analysis of DNA complementary to three mouseM r-68,000 heat shock protein mRNAs. J Biol Chem 261:2102–2112
MacDermott AB, Mayer ML, Westbrook GL, Smith SJ, Barker JL (1986) NMDA-receptor activation increases cytoplasmic calcium concentration in cultured spinal neurons. Nature 321:519–522
Magnusson KG, Wieloch TW (1987) Early loss of ubiquitinprotein conjugate immunoreactivity precedes delayed neuronal death in the rat hippocampus following transient cerebral ischemia. Soc Neurosci Abstr 13:921
McMaster WR, Williams AF (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen. Eur J Immunol 9:426–433
Morgan JI, Curran T (1986) Role of ion flux in the control of c-fos expression. Nature 322:552–555
Morgan JI, Cohen DR, Hempstead JL, Curran T (1987) Mapping patterns of c-fos expression in the central nervous system after seizure. Science 237:192–197
Munro S, Pelham HRB (1986) An hsp70-like protein in the ER: identity with the 78-kDa glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell 46:291–300
Nowak TS Jr (1985) Synthesis of a stress protein following transient ischemia in the gerbil. J Neurochem 45:1635–1641
Nowak TS Jr, Carty ER, Lust WD, Passonneau JV (1984) An in vitro amino acid incorporation method for assessing the status of in vivo protein synthesis. Anal Biochem 136:285–292
Nowak TS Jr, Vass K, Welch WJ (1987) Localization of 70-kDa heat shock protein induction in gerbil brain after transient ischemia. Soc Neurosci Abstr 13:1688
O'Malley K, Mauron A, Barchas JD, Kedes L (1985) Constitutively expressed rat mRNA encoding a 70-kilodalton heat shock-like protein. Mol Cell Biol 5:3476–3483
Pelham HRB (1986) Speculations on the functions of the major heat shock and glucose-regulated proteins. Cell 46:959–961
Pulsinelli WA, Brierley JB, Plum F (1982) Temporal profile of neuronal damage in a model of transient forebrain ischemia. Ann Neurol 11:491–498
Schlesinger MJ, Ashburner M, Tissières A (1982) Heat shock from bacteria to man. Cold Spring Harbor Laboratory, Cold Spring Harbor
Simon RR, Swan JH, Griffiths T, Meldrum BS (1984) Blockade ofN-methyl-d-aspartate receptors may protect against ischemic damage in the brain. Science 226:850–852
Suzuki R, Yamaguchi T, Li CL, Klatzo I (1983) The effect of 5-minute ischemia in Mongolian gerbils. II. Changes of spontaneous neuronal activity in cerebral cortex and CA1 sector of hippocampus. Acta Neuropathol (Berl) 60:217–222
Thilmann R, Xie Y, Kleihues P, Kiessling M (1986) Persistent inhibition of protein synthesis precedes delayed neuronal death in postischemic gerbil hippocampus. Acta Neuropathol (Berl) 71:88–93
Towbin H, Staehelin T, Gordon I (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
Ungewickel E (1985) The 70-kDa mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases triskelion from coated vesicles. EMBO J 4:3385–3391
Welch WJ, Feramisco JR (1984) Nuclear and nucleolar localization of the 72,000-dalton heat shock protein in heat-shocked mammalian cells. J Biol Chem 259:4501–4513
Welch WJ, Feramisco JR (1985) Rapid purification of mammalian 70,000-dalton stress proteins: affinity of the proteins for nucleotides. Mol Cell Biol 5:1229–1237
Welch WJ, Suhan JP (1986) Cellular and biochemical events in mammalian cells during and after recovery from physiological stress. J Cell Biol 103:2035–2052
Whatley SA, Leung T, Hall C, Lim L (1986) The brain 69-kilodalton microtubule-associated protein is a cognate form of the 70-kilodalton mammalian heat-shock protein and is present as a specific isoform in synaptosomal membranes. J Neurochem 47:1576–1583
Wieloch T, Lindvall O, Blomquist P, Gage F (1985) Evidence for amelioration of ischaemicneuronal damage in the hippocampal formation by lesions of the perforant path. Neurol Res 7:24–27