Evidence of Trypanosoma cruzi II infection in Colombian chagasic patients

Parasitology Research - Tập 103 - Trang 731-734 - 2008
German Zafra1, Julio Cesar Mantilla1, Helder Magno Valadares2, Andrea Mara Macedo2, Clara Isabel González1,3
1Grupo de Inmunología y Epidemiología Molecular-GIEM, Facultad de Salud, Universidad Industrial de Santander, Bucaramanga, Colombia
2Departamento de Bioquímica e Imunología, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil
3Escuela de Bacteriología, Facultad de Salud, Bucaramanga, Colombia

Tóm tắt

Trypanosoma cruzi is genetically classified into at least two major lineages named T. cruzi I (also named Tc I) and T. cruzi II (also named Tc IIb). T. cruzi II is associated with Chagas’ disease in the southern cone of South America, while T. cruzi I is the only one so far identified in chagasic patients of Central America and in the northern part of South America. Herein we identified T. cruzi IIb directly in 9.9% of blood of chronic chagasic patients of Colombia. This finding establishes that in this region, the two T. cruzi lineages are associated with the pathology of Chagas’ disease and have implications in the morbidity and epidemiology of the disease.

Tài liệu tham khảo

Andrade LO, Machado CR, Chiari E, Pena SD, Macedo AM (1999) Differential tissue distribution of diverse clones of Trypanosoma cruzi in infected mice. Mol Biochem Parasitol 100:163–172 Anonymous (1999) Recommendations from a satellite meeting. Mem Inst Oswaldo Cruz 94:429–432 Añez N, Crisante G, da Silva FM, Rojas A, Carrasco H, Umezawa ES, Stolf AM, Ramírez JL, Teixeira MM (2004) Predominance of lineage I among Trypanosoma cruzi isolates from Venezuelan patients with different clinical profiles of acute Chagas’ disease. Trop Med Int Health 9:1319–1326 Araujo CA, Cabello PH, Jansen AM (2007) Growth behaviour of two Trypanosoma cruzi strains in single and mixed infections: in vitro and the intestinal tract of the blood-sucking bug, Triatoma brasiliensis. Acta Trop 101:225–231 Avila HA, Pereira JB, Thiemann O, De Paiva E, Degrave W, Morel C, Simpson L (1993) Detection of Trypanosoma cruzi in blood specimens of chronic chagasic patients by polymerase chain reaction amplification of kinetoplast minicircle DNA: comparison with serology and xenodiagnosis. J Clin Microbiol 31:2421–2426 Brisse S, Verhoef J, Tibayrenc M (2001) Characterization of large and small subunit rRNA and mini-exon genes further supports the distinction of six Trypanosoma cruzi lineages. Int J Parasitol 31:1218–1226 Cuervo P, Cupolillo E, Segura I, Saravia N, Fernandes O (2002) Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA. Mem Inst Oswaldo Cruz 97:877–880 Devia F (1999) Caracterización bioquímica de cepas de Trypanosoma cruzi del departamento de Santander y otros departamentos. Bogotá. Tesis de grado Magister en Microbiología. Universidad de los Andes Dias JCP (1992) Epidemiology of Chagas disease. In: Welden S et al (ed) Chagas’ disease (American Trypanosomiasis): its impact on transfusion and clinical medicine. International Society of Blood Transfusion, Brazil, pp 49–80 Freitas JM, Augusto-Pinto L, Pimenta J, Bastos Rodrigues L, Gonçalves VF, Teixeira SMR, Chiari E, Junqueira ACV, Fernandes O, Macedo AM, Machado CR, Pena SDJ (2006) Ancestral genomes, sex, and the population structure of Trypanosoma cruzi. PLoS pathogens 2:e24 Gutierrez R, Angulo VM, Tarazona Z, Britto C, Fernandes O (2004) Comparison of four serological test for the diagnosis of Chagas disease in a Colombian endemic area. Parasitology 129:439–444 Higo H, Miura S, Horio M, Mimori T, Hamano S, Agatsuma T, Yanagi T, Cruz-Reyes A, Uyema N, Rojas de Arias A, Matta V, Akahane H, Hirayama K, Takeuchi T, Tada I, Himeno K (2004) Genotypic variation among lineages of Trypanosoma cruzi and its geographic aspects. Parasitol Int 53:337–344 Luna KP, Jaramillo CL, Hernández J, Angulo VM (2007) Variabilidad genética de aislados de Trypanosoma cruzi I por medio de ITS-RFLP en Santander Colombia. Bol Malariol Salud Amb 47:139 Macedo AM, Oliveira RP, Pena SD (2002) Chagas disease: role of parasite genetic variation in pathogenesis. Expert Rev Mol Med 4:1–16 Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16:1215 Moncayo A (2003) Chagas disease: current epidemiological trends after the interruption of vectorial and transfusional transmission in the Southern Cone countries. Mem Inst Oswaldo Cruz 98:577–591 Montilla MM, Guhl F, Jaramillo C, Nicholls S, Barnabé C, Bosseno MF, Breniere SF (2002) Isoenzyme clustering of trypanosomatidae Colombian populations. Am J Trop Med Hyg 66:394–400 Solari A, Campillay R, Ortíz S, Wallace A (2001) Identification of Trypanosoma cruzi genotypes circulating in Chilean chagasic patients. Exp Parasitol 97:226–233 Souto RP, Fernandes O, Macedo AM, Campbell DA, Zingales B (1996) DNA markers define two major phylogenetic lineages of Trypanosoma cruzi. Mol Biochem Parasitol 83:141–152 Triana O, Ortiz S, Dujardin JC, Solari A (2006) Trypanosoma cruzi determined by molecular karyotype and minicircle Southern blot analysis. Exp Parasitol 113:62–66 Zingales B, Souto RP, Mangia RH, Lisboa CV, Campbell DA, Coura JR, Jansen A, Fernandes O (1998) Molecular epidemiology of American trypanosomiasis in Brazil based on dimorphisms of rRNA and mini-exon gene sequences. Intern J Parasitol 28:105–112