Analysis of virus-specific RNA species and proteins in Freon-113 preparations of the Borna disease virus

Springer Science and Business Media LLC - Tập 182 - Trang 271-280 - 1993
J. A. Rieht1, J. E. Clements2, S. Herzog1, J. Pyper2, K. Wahn1, H. Becht1
1Institut für Virologie, Justus-Liebig-Universität Giessen, Giessen, Germany
2Division of Comparative Medicine, The Johns Hopkins University, School of Medicine, Retrovirus Biology Laboratories, Baltimore, USA

Tóm tắt

Treatment of homogenates from Borna disease virus (BDV)-infected brain tissue or cell cultures with Freon-113 yielded infectious particles with a buoyant density of 1.16–1.22 g/ml. Positive- and negative-stranded BDV-specific RNA species as well as three virus-specific proteins, known to be present in BDV-infected cell extracts, were demonstrated in these Freon-treated fractions. When the Freon-purified virus preparations were treated with RNase A prior to RNA extraction, only negative-stranded, genomic RNA was detected in Northern blot hybridizations using sense and antisense RNA probes. These data substantiate that BDV is a negative-stranded RNA virus.

Tài liệu tham khảo

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