Calcium sustained release, pH changes and cell viability induced by chitosan-based pastes for apexification

Shigaku = Odontology - Tập 107 - Trang 223-230 - 2018
Juan Carlos Flores-Arriaga1, Amaury de Jesús Pozos-Guillén2, Omar González-Ortega2, Diana María Escobar-García2, David Masuoka-Ito3, Bryan Iván Martín del Campo-Téllez3, Bernardino Isaac Cerda-Cristerna4
1Universidad Nacional Autónoma de México Unidad León, Guanajuato, Mexico
2Universidad Autónoma de San Luis Potosí, Zona Universitaria, San Luis Potosí, Mexico
3Universidad Autónoma de Aguascalientes, Zona Universitaria, Aguascalientes, Mexico
4Universidad Veracruzana, Región Orizaba-Córdoba, Veracruz, Mexico

Tóm tắt

We explored chitosan-based sustained release pastes for apexification. The study aimed to formulate chitosan-based pastes loaded with calcium hydroxide (CH) or with calcium chloride (CC), and to evaluate the sustained release of Ca2+ and pH changes in deionized water as well as the effect of the pastes on cell viability. The pastes were formulated by dissolution of the chitosan in 1% or 2% acetic acid (AAC) plus the addition of CH or CC, then were suspended in deionized water for 50 days; the released Ca(II) and pH were measured with an electrode probe. The effect of the pastes on viability of human dental pulp cells was evaluated with a MTS assay. The results showed that the pastes prepared with 1% and 2% AAC and loaded with CH released a 74.9% and a 76.1% of the Ca2+ content, respectively, while the pastes prepared with 1% and 2% AAC loaded with CC released a content of Ca2+ of 90.8% and 76.6%, respectively. A control paste (CH and polyethylene glycol) released a 95.4%; significant statistical differences were found between the percentage of the experimental pastes and the control. The CH-loaded pastes caused an alkaline pH at the starting of the study, but the pH became neutral at the ending. The pH of the CC-loaded pastes was neutral at the starting and was acid at the ending. The pastes no affected on the cell viability. The chitosan-based pastes showed a suitable sustained release profile and cytocompatibility.

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