Species delimitation based on mtDNA genes suggests the occurrence of new species of Mesocestoides in the Mediterranean region
Parasites and Vectors - 2018
Tóm tắt
This study is the first contribution to the molecular taxonomy of Mesocestoides spp. from domestic and wild carnivores in the Mediterranean area. A total of 13 adult worms and 13 larval stages of Mesocestoides spp. were collected from domestic and wild carnivore hosts in Italy and Tunisia. Samples collected in the Slovak Republic were used as comparative samples from outside the Mediterranean. The genes cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) of the mitochondrial genome were used as molecular markers to investigate the presence of cryptic Mesocestoides species in the area analysed. Results were consistent in showing three well-supported clusters of Mesocestoides spp. in southern Italy and Tunisia, which were strongly divergent from Mesocestoides litteratus, M. corti and M. lineatus. High levels of genetic variation and no evidence of geographical structuring was found between the clusters. Considering the low dispersal capability of the intermediate hosts of Mesocestoides spp., the lack of geographical structuring among the Mediterranean regions could be due to a high potential for dispersion of the definitive hosts. This study provides a foundation for future formal descriptions of new species of the genus Mesocestoides in the Mediterranean area.
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Tài liệu tham khảo
Loos-Frank B. One or two intermediate hosts in the life cycle of Mesocestoides (Cyclophyllidae, Mesocestoididae)? Parasitol Res. 1991;77:726–8.
Papini R, Matteini A, Bandinelli P, Pampurini F, Mancianti F. Effectiveness of praziquantel for treatment of peritoneal larval cestodiasis in dogs: a case report. Vet Parasitol. 2010;170:158–61.
McAllister CT, Bruce Conn D, Freed PS, Burdick DA. A new host and locality record for Mesocestoides sp. tetrathyridia (Cestoidea: Cyclophyllidea), with a summary of the genus from snakes of the world. J Parasitol. 1991;77:329–31.
Literák I, Olson PD, Georgiev BB, Špakulová M. First record of metacestodes of Mesocestoides sp. in the common starling (Sturnus vulgaris) in Europe, with an 18S rDNA characterization of the isolate. Folia Parasitol. 2004;51:45–9.
Hrčkova G, Miterpáková M, O’Connor A, Šnábel V, Olson P. Molecular and morphological circumscription of Mesocestoides tapeworms from red foxes (Vulpes vulpes) in central Europe. Parasitology. 2011;138:638–47.
Lahmar S, Boufana B, Ben Boubaker S, Landolsi F. Intestinal helminths of golden jackals and red foxes from Tunisia. Vet Parasitol. 2014;204:297–303.
Dalimi A, Sattari A, Motamedi G. A study on intestinal helminthes of dogs, foxes and jackals in the western part of Iran. Vet Parasitol. 2006;142:129–33.
Calvete C, Lucientes J, Castillo JA, Estrada R, Gracia MJ, Peribáñez MA, Ferrer M. Gastrointestinal helminth parasites in stray cats from the mid-Ebro Valley, Spain. Vet Parasitol. 1998;75:235–40.
Karamon J, Dabrowska J, Kochanowski M, Samorek-Pieróg M, Sroka J, Różycki M, Bilska-Zajac E, Zdybel J, Cencek T. Prevalence of intestinal helminths of red foxes (Vulpes vulpes) in central Europe (Poland): a significant zoonotic threat. Parasit Vectors. 2018;11:436.
Crosbie PR, Boyce WM, Platzer EG, Nadler SA, Kerner C. Diagnostic procedures and treatment of eleven dogs with peritoneal infections caused by Mesocestoides spp. J Am Vet Med Assoc. 1998;213:1578–83.
Siles-Lucas M, Hemphill A. Cestode parasites: application of in vivo and in vitro models for studies on the host-parasite relationship. Adv Parasitol. 2002;51:133–230.
Boyce W, Shender L, Schultz L, Vickers W, Johnson C, Ziccardi M, et al. Survival analysis of dogs diagnosed with canine peritoneal larval cestodiasis (Mesocestoides spp.). Vet Parasitol. 2011;180:256–61.
Montalbano Di Filippo M, Meoli R, Cavallero S, Eleni C, De Liberato C, Berrilli F. Molecular identification of Mesocestoides sp. metacestodes in a captive gold-handed tamarin (Saguinus midas). Infect Genet Evol. 2018;65:399–405.
Fuentes MV, Galán-Puchades T, Malone JB. A new case report of human Mesocestoides infection in the United States. Am J Trop Med Hyg. 2003;68:566–7.
Széll Z, Tolnai Z, Sréter T. Environmental determinants of the spatial distribution of Mesocestoides spp. and sensitivity of flotation method for the diagnosis of mesocestoidosis. Vet Parasitol. 2015;212:427–30.
Zaleśny G, Hildebrand J. Molecular identification of Mesocestoides spp. from intermediate hosts (rodents) in central Europe (Poland). Parasitol Res. 2012;110:1055–61.
Tenora F. Mesocestoides litteratus (Batsch, 1786) (Cestoda), parasite of Vulpes vulpes (L., 1758) (Carnivora) in the Czech Republic. Acta Univ Agric Silvic Mendel Brun. 2005;53:185–8.
Skrjabin KI. Tetrabotrididae and Mesocestoididae - platyhelminths of birds and mammals. In: Skrjabin KI, editor. Osnovy Cestodologii, Volume 9. Moscow: Nauka; 1978. p. 156–215. (In Russian).
Jančev J. Morphology, taxonomy and distribution of the species of genus Mesocestoides Vaillant, 1863 in Bulgaria. Khelmintologiya. 1986;21:45–65.
De Jong Y, Verbeek M, Michelsen V, Bjørn PP, Los W, Steeman F, et al. Fauna Europaea - all European animal species on the web. Biodivers Data J. 2014;2:e4034.
Andras T, Peter T. Data on worm infestation cats (Felis catus) in Hungarian hunting areas. Magy Allatorvosok Lapja. 2002;124:26–30.
Segovia JM, Guerrero R, Torres J, Miguel J, Feliu C. Ecological analyses of the intestinal helminth communities of the wolf, Canis lupus, in Spain. Folia Parasitol. 2003;50:231–6.
Martinez-Carrasco C, Ruiz De Ybanez MR, Sagarminaga JL, Garijo MM, Moreno F, Acosta I, et al. Parasites of the red fox (Vulpes vulpes Linneus, 1758) in Murcia, southeast Spain. Rev Med Vet. 2007;158:331–5.
Krone O, Guminsky O, Meinig H, Hermann M, Trinzen M, Wibbelt G. Endoparasite spectrum of wild cats (Felis silvestris Schreber, 1777) and domestic cats (Felis catus L.) from the Eifel, Pfalz region and Saarland, Germany. Eur J Wildl Res. 2008;54:95–100.
Magi M, Macchioni F, Dell’Omodarme M, Prati MC, Calderini P, Gabrielli S, et al. Endoparasites of red fox (Vulpes vulpes) in central Italy. J Wildl Dis. 2009;45:881–5.
Bonfanti U, Bertazzolo W, Pagliaro L, Demarco B, Venco L, Casiraghi M, Bandi C. Clinical, cytological and molecular evidence of Mesocestoides sp. infection in a dog from Italy. J Vet Med A Physiol Pathol Clin Med. 2004;51:435–8.
Lanteri G, Di Caro G, Capucchio MT, Gaglio G, Reina V, Lo Giudice C, Zanet S, Marino F. Mesocestoidosis and multivisceral tetrathyridiosis in a European cat. Vet Med Czech. 2017;62:356–62.
Otranto D, Varcasia A, Solinas C, Scala A, Brianti E, Dantas-Torres F, et al. Redescription of Cercopithifilaria bainae Almeida & Vicente, 1984 (Spirurida, Onchocercidae) from a dog in Sardinia, Italy. Parasit Vectors. 2013;6:132.
Littlewood DTJ, Waeschenbach A, Nikolov PN. In search of mitochondrial markers for resolving the phylogeny of cyclophyllidea tapeworms (Platyhelminthes, Cestoda) - a test study with Davaineidae. Acta Parasitol. 2008;53:133–44.
Sanna D, Dedola GL, Lai T, Curini-Galletti M, Casu M. PCR-RFLP: a practical method for the identification of specimens of Patella ulyssiponensis s.l. (Gastropoda: Patellidae). Ital J Zool. 2012;79:50–9.
Hall TA. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999;41:95–8.
Librado P, Rozas J. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009;25:1451–2.
Bandelt HJ, Forster P, Rohl A. Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol. 1999;16:37–48.
Clement M, Posada D, Crandall K. TCS: a computer program to estimate gene genealogies. Mol Ecol. 2000;9:1657–60.
Corander J, Cheng L, Marttinen P, Sirén J, Tang JBAPS. Bayesian analysis of population structure V. 6.0. Department of Mathematics and statistics. Helsinki: University of Helsinki; 2013.
Kashiide T, Matsumoto J, Yamaya Y, Uwasawa A, Miyoshi A, Yamada K, et al. Case report: first confirmed case of canine peritoneal larval cestodiasis caused by Mesocestoides vogae (syn. M. corti) in Japan. Vet Parasitol. 2014;201:154–7.
Lesniak I, Heckmann I, Heitlinger E, Szentiks CA, Nowak C, Harms V, et al. Population expansion and individual age affect endoparasite richness and diversity in a recolonising large carnivore population. Sci Rep. 2017;7:41730.
Strimmer K, Von Haeseler A. Quartet puzzling: a quartet maximum-likelihood method for reconstructing tree topologies. Mol Biol Evol. 1996;13:964–9.
Schmidt HA, Strimmer K, Vingron M, von Haeseler A. TREE-PUZZLE: maximum likelihood phylogenetic analysis using quartets and parallel computing. Bioinformatics. 2002;18:502–4.
Schmidt HA, von Haeseler A. Phylogenetic inference using maximum likelihood methods. In: Lemey P, Salemi M, Vandamme AM, editors. The Phylogenetic Handbook. 5th ed. Cambridge: Cambridge University Press; 2012. p. 181–209.
Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for bigger datasets. Mol Biol Evol. 2016;33:1870–4.
Hebert PDN, Cywinska A, Ball SL, deWaard JR. Biological identifications through DNA barcodes. Proc Biol Sci. 2003;270:313–22.
Scarpa F, Cossu P, Lai T, Sanna D, Curini-Galletti M, Casu M. Meiofaunal cryptic species challenge species delimitation: the case of the Monocelis lineata (Platyhelminthes: Proseriata) species complex. Contrib Zool. 2016;85:123–45.
Scarpa F, Cossu P, Sanna D, Lai T, Casu M, Curini-Galletti M. New insights on the genus Otoplana Du Plessis, 1889 (Platyhelminthes: Proseriata), with description of two new species from the Canary Islands. Mar Biodivers. 2017. https://doi.org/10.1007/s12526-017-0785-1.
Scarpa F, Sanna D, Cossu P, Lai T, Casu M, Curini-Galletti M. How to achieve internal fertilization without a vagina: the study case of the genus Archilina Ax, 1959 (Platyhelminthes, Proseriata) from Canary Islands. Mar Biodivers. (In Press) https://doi.org/10.1007/s12526-018-0890-9.
Jabbar A, Papini R, Ferrini N, Gasser RB. Use of a molecular approach for the definitive diagnosis of proliferative larval mesocestoidiasis in a cat. Infect Genet Evol. 2012;12:1377–80.
Varcasia A, Tamponi C, Tosciri G, Pipia AP, Dore F, Schuster RK, et al. Is the red fox (Vulpes vulpes) a competent definitive host for Taenia multiceps? Parasit Vectors. 2015;8:491.
Varcasia A, Canu S, Lightowlers MW, Scala A, Garippa G. Molecular characterization of Echinococcus granulosus strains in Sardinia. Parasitol Res. 2006;98:273–7.
Saarma U, Jõgisalu I, Moks E, Varcasia A, Lavikainen A, Oksanen A, et al. A novel phylogeny for the genus Echinococcus, based on nuclear data, challenges relationships based on mitochondrial evidence. Parasitology. 2009;136:317–28.
Varcasia A, Jia WZ, Yan HB, Manunta ML, Pipia AP, Garippa G, et al. Molecular characterization of subcutaneous and muscular coenurosis of goats in United Arab Emirates. Vet Parasitol. 2012;190:604–7.
Boufana B, Scala A, Lahmar S, Pointing S, Craig PS, Dessì G, et al. A preliminary investigation into the genetic variation and population structure of Taenia hydatigena from Sardinia, Italy. Vet Parasitol. 2015;214:67–74.
Scala A., Pipia AP, Dore F, Sanna G, Tamponi C, Marrosu R, et al. Epidemiological updates and economic losses due to Taenia hydatigena in sheep from Sardinia, Italy. Parasitol Res. 2015;114:3137–43.
Kinkar L, Laurimaë T, Simsek S, Balkaya I, Casulli A, Manfredi MT, et al. High-resolution phylogeography of zoonotic tapeworm Echinococcus granulosus sensu stricto genotype G1 with an emphasis on its distribution in Turkey, Italy and Spain. Parasitology. 2016;143:1790–801.
Jörger KM, Schrödl M. How to describe a cryptic species? Practical challenges of molecular taxonomy. Front Zool. 2013;10:59.
Dayrat B. Toward integrative taxonomy. Biol J Linnean Soc. 2005;85:407–15.