Plant tissue-specific promoters can drive gene expression in Escherichia coli

Plant Cell, Tissue and Organ Culture - Tập 113 - Trang 387-396 - 2012
Martin Jopcik1, Miroslav Bauer2,3, Jana Moravcikova1, Eva Boszoradova1, Ildiko Matusikova1, Jana Libantova1
1Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovak Republic
2Animal Production Research Centre, Nitra, Slovak Republic
3Constantine the Philosopher University, Nitra, Slovak Republic

Tóm tắt

Plant transgenesis often requires the use of tissue-specific promoters to drive the transgene expression exclusively in targeted tissues. Although the eukaryotic promoters are expected to stay silent in Escherichia coli, when the promoter-transgene units within the plant transformation vectors are constructed and propagated, some eukaryotic promoters have been reported to be active in prokaryotes. The potential activity of plant promoter in E. coli cells should be considered in cases of expression of proteins that are toxic for host cells, environmental risk assessment or the stability in E. coli of plant vectors for specific Cre/loxP applications. In this study, DNA fragments harbouring four embryo- and/or pollen-specific Arabidopsis thaliana promoters were investigated for their ability to drive heterologous gene expression in E. coli cells. For this, they were fused to gfp:gus reporter genes in the pCAMBIA1304 vector. Although BPROM, bacterial sigma70 promoter recognition program identified several sequences with characteristics similar to bacterial promoters including -10 and -35 sequences in each of tested fragments, the experimental approach showed that only one promoter fragment was able to drive relatively strong- and one promoter fragment relatively weak-GUS expression in E. coli cells. Remaining two tested promoters did not drive any transgene expression in bacteria. Our results also showed that cloning of a shorter plant promoter sequence into vectors containing lacZ α-complementation system can increase the probability of gene expression driven by upstream located lac promoter. This should be considered when cloning of plant expression units, the expression of which is unwanted in E. coli.

Tài liệu tham khảo

Altermann E, Russell WM, Azcarate-Peril MA, Barrangou R, Buck BL, McAuliffe O, Souther N, Dobson A, Duong T, Callanan M, Lick S, Hamrick A, Cano R, Klaenhammer TR (2005) Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM. Proc Natl Acad Sci USA 102:3906–3912 Antonucci TK, Wen P, Rutter WJ (1989) Eukaryotic promoters drive gene expression in Escherichia coli. J Biol Chem 264:17656–17659 Ballester A, Cervera M, Pena L (2010) Selectable marker-free transgenic orange plants recovered under non-selective conditions and through PCR analysis of all regenerants. Plant Cell Tiss Organ Cult 102:329–336 Barrett C, Cobb E, McNicol R, Lyon G (1997) A risk assessment study of plant genetic transformation using Agrobacterium and implications for analysis of transgenic plants. Plant Cell Tiss Organ Cult 47:135–144 Bertolla F, Simonet P (1999) Horizontal gene transfers in the environment: natural transformation as a putative process for gene transfers between transgenic plants and microorganisms. Res Microbiol 150:375–384 Bertolla F, VanGijsegem F, Nesme X, Simonet P (1997) Conditions for natural transformation of Ralstonia solanacearum. Appl Environ Microbiol 63:4965–4968 Bihao C, Xiaosan W, Jianjun L, Xiou X, Qinhua C (2012) Inducing male sterility of tomato using two component system. Plant Cell, Tissue Organ Cult 111(2):163–172 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254 Casson S, Spencer M, Walker K, Lindsey K (2005) Laser capture microdissection for the analysis of gene expression during embryogenesis of Arabidopsis. Plant J 42:111–123 Chen HY, Bjerknes M, Kumar R, Jay E (1994) Determination of the optimal aligned spacing between the Shine-Dalgarno sequence and the translation initiation codon of Escherichia coli mRNAs. Nucleic Acids Res 22:4953–4957 Clancy S, Brown W (2008) Translation: DNA to mRNA to protein. Nat Educ www.nature.com/scitable/topicpage/Translation-DNA-to-mRNA-to-Protein-393 Deana A, Belasco JG (2005) Lost in translation: the influence of ribosomes on bacterial mRNA decay. Gen Dev 19:2526–2533 Drea SC, Lao NT, Wolfe KH, Kavanagh TA (2006) Gene duplication, exon gain and neofunctionalization of OEP16-related genes in land plants. Plant J 46:723–735 Feklistov A, Darst SA (2011) Structural basis for promoter-10 element recognition by the bacterial RNA polymerase sigma subunit. Cell 147(6):1257–1269 Feng L, Niu DK (2007) Relationship between mRNA stability and length: an old question with a new twist. Biochem Genet 45:131–137 Fraser PD, Romer S, Shipton CA, Mills PB, Kiano JW, Misawa N, Drake RG, Schuch W, Bramley PM (2002) Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner. Proc Natl Acad Sci USA 99(2):1092–1097 French S, Levy-Booth D, Samarajeewa A, Shannon KE, Smith J, Trevors JT (2009) Elevated temperatures and carbon dioxide concentrations: effects on selected microbial activities in temperate agricultural soils. World Microbiol Biotechnol 25:1887–1900 Gilbertson L (2003) Cre-lox recombination: cre-active tools for plant biotechnology. Trends Biotechnol 21:550–555 Goussard S, Grillot-Courvalin C, Courvalin P (2003) Eukaryotic promoters can direct protein synthesis in gram negative bacteria. J Mol Microbiol Biotechnol 6:211–218 Guldener U, Heck S, Fiedler T, Beinhauer J, Hegemann JH (1996) A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res 24(13):2519–2524 Harley CB, Reynolds RP (1987) Analysis of Escherichia coli promoter sequences. Nucleic Acids Res 15:2343–2361 Heritage J (2004) The fate of transgenes in the human gut. Nat Biotechnol 22:170–172 Heritage J (2005) Transgenes for tea? Trends Biotechnol 23:17–21 Honys D, Twell D (2004) Transcriptome analysis of haploid male gametophyte development in Arabidopsis. Genome Biol 5:11 Jacob D, Lewin A, Meister B, Appel B (2002) Plant-specific promoter sequences carry elements that are recognised by the eubacterial transcription machinery. Transgenic Res 11:291–303 Kaufusi PH, Forsberg LS, Tittabutr P, Borthakur D (2004) Regulation of exopolysaccharide synthesis in Rhizobium sp strain TAL1145 involves an alternative sigma factor gene, rpoH2. Microbiology-Sgm 150:3473–3482 Kay E, Vogel TM, Bertolla F, Nalin R, Simonet P (2002) In situ transfer of antibiotic resistance genes from transgenic (transplastomic) tobacco plants to bacteria. Appl Environ Microbiol 68:3345–3351 Lewin A, Jacob D, Freytag B, Appel B (1998) Gene expression in bacteria directed by plant-specific regulatory sequences. Transgenic Res 7:403–411 Lewin A, Tran TT, Jacob D, Mayer M, Freytag B, Appel B (2004) Yeast DNA sequences initiating gene expression in Escherichia coli. Microbiol Res 159:19–28 Li DS, Aaskov J, Lott WB (2011) Identification of a cryptic prokaryotic promoter within the cDNA encoding the 5’ end of dengue virus RNA genome. PLoS ONE 6(3):e18197. doi:10.1371/journal.pone.0018197 Messeguer J (2003) Gene flow assessment in transgenic plants. Plant Cell Tiss Organ Cult 73:201–212 Messing J (1998) Plant science in lac: a continuation of using tools from Escherichia coli in studying gene function in heterologous systems. Proc Natl Acad Sci USA 95:93–94 Moravcikova J, Vaculkova E, Bauer M, Libantova J (2008) Feasibility of the seed specific cruciferin C promoter in the self excision Cre/loxP strategy focused on generation of marker-free transgenic plants. Theor Appl Genet 117:1325–1334 O′Neill MC (1989) Escherichia coli promoters 1. Consensus as it relates to spacing class, specificity, repeat substructure, and 3-dimensional organization. J Biol Chem 264:5522–5530 Petri C, Lopez-Noguera S, Wang H, Garcia-Almodovar C, Alburquerque N, Burgos L (2012) A chemical-inducible Cre-LoxP system allows for elimination of selection marker genes in transgenic apricot. Plant Cell, Tissue Organ Cult 110(3):337–346 Pribnow D (1975) Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc Natl Acad Sci USA 72(3):784–788 Qin Y, Leydon AR, Manziello A, Pandey R, Mount D, Denic S, Vasic B, Johnson MA, Palanivelu R (2009) Penetration of the stigma and style elicits a novel transcriptome in pollen tubes, pointing to genes critical for growth in a pistil. PLoS Genet 5:8 Qiu CX, Sangha JS, Song FS, Zhou ZY, Yin A, Gu KY, Tian DS, Yang JB, Yin ZC (2010) Production of marker-free transgenic rice expressing tissue-specific Bt gene. Plant Cell Rep 29:1097–1107 Rao MVR, Parameswari C, Sripriya R, Veluthambi K (2011) Transgene stacking and marker elimination in transgenic rice by sequential Agrobacterium-mediated co-transformation with the same selectable marker gene. Plant Cell Rep 30:1241–1252 Roberts CS, Rajagopal S, Smith LA, Nguyen TA, Yang W, Nugroho S, Ravi KS, Vijayachandra K, Harcourt KL, Dransfield L, Desamero N, Hajdukiewicz P, Svab Z, Maliga P, Mayer JE, Keese P, Kilian K, Jefferson RA (2002) A comprehensive set of modular vectors for advanced manipulations and efficient transformation of plants by both Agrobacterium and direct DNA uptake methods. CAMBIA, Canberra, Australia. http://www.cambia.org Romanowski G, Lorenz MG, Wackernagel W (1993) Use of polymerase chain-reaction and electroporation of Escherichia coli to monitor the persistance of extracellular plasmid DNA introduced into natural soils. Appl Environ Microbiol 59:3438–3446 Roque E, Gomez MD, Ellul P, Wallbraun M, Madueno F, Beltran JP, Canas LA (2007) The PsEND1 promoter: a novel tool to produce genetically engineered male-sterile plants by early anther ablation. Plant Cell Rep 26(3):313–325 Roy SD, Saxena M, Bhomkar PS, Pooggin M, Hohn T, Bhalla-Sarin N (2008) Generation of marker free salt tolerant transgenic plants of Arabidopsis thaliana using the gly I gene and cre gene under inducible promoters. Plant Cell Tiss Organ Cult 95:1–11 Smirnova OG, Ibragimova SS, Kochetov AV (2012) Simple database to select promoters for plant transgenesis. Transgenic Res 21(2):429–437 Sorensen HP, Mortensen KK (2005) Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli. Microb Cell Fact 4(1): doi:10.1186/1475-2859-1184-1181 Strohl WR (1992) Compilation and analysis of DNA sequences associated with apparent streptomycete promoters. Nucleic Acids Res 20:961–974 Studier FW (2005) Protein production by auto-induction in high-density shaking cultures. Protein Expres Purif 41(1):207–234 Sun QY, Ding LW, He LL, Sun YB, Shao JL, Luo M, Xu ZF (2009) Culture of Escherichia coli in SOC medium improves the cloning efficiency of toxic protein genes. Anal Biochem 394(1):144–146 Vickers CE, Xue GP, Gresshoff PM (2006) A novel cis-acting element, ESP, contributes to high-level endosperm-specific expression in an oat globulin promoter. Plant Mol Biol 62(1–2):195–214 Wach A, Brachat A, Pohlmann R, Philippsen P (1994) New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10(13):1793–1808 Wang YJ, Yau YY, Perkins-Balding D, Thomson JG (2011) Recombinase technology: applications and possibilities. Plant Cell Rep 30:267–285