Establishment of a Quantitative Polymerase Chain Reaction Assay for Monitoring Chimeric Antigen Receptor T Cells in Peripheral Blood

Curran, 2012, Chimeric antigen receptors for T cell immunotherapy: current understanding and future directions, J Gene Med, 14, 405, 10.1002/jgm.2604 Jena, 2010, Redirecting T-cell specificity by introducing a tumor-specific chimeric antigen receptor, Blood, 116, 1035, 10.1182/blood-2010-01-043737 Lipowska-Bhalla, 2012, Targeted immunotherapy of cancer with CAR T cells: achievements and challenges, Cancer Immunol Immunother, 61, 953, 10.1007/s00262-012-1254-0 Grupp, 2013, Chimeric antigen receptor-modified T cells for acute lymphoid leukemia, N Engl J Med, 368, 1509, 10.1056/NEJMoa1215134 Faitschuk, 2016, Chimeric antigen receptor T cells targeting Fc mu receptor selectively eliminate CLL cells while sparing healthy B cells, Blood, 128, 1711, 10.1182/blood-2016-01-692046 Atanackovic, 2016, Chimeric Antigen Receptor (CAR) therapy for multiple myeloma, Br J Haematol, 172, 685, 10.1111/bjh.13889 Savoldo, 2007, Epstein Barr virus specific cytotoxic T lymphocytes expressing the anti-CD30zeta artificial chimeric T-cell receptor for immunotherapy of Hodgkin disease, Blood, 110, 2620, 10.1182/blood-2006-11-059139 Turtle, 2016, Immunotherapy of non-Hodgkin's lymphoma with a defined ratio of CD8+ and CD4+ CD19-specific chimeric antigen receptor-modified T cells, Sci Transl Med, 8, 355ra116, 10.1126/scitranslmed.aaf8621 Torikai, 2012, A foundation for universal T-cell based immunotherapy: T cells engineered to express a CD19-specific chimeric-antigen-receptor and eliminate expression of endogenous TCR, Blood, 119, 5697, 10.1182/blood-2012-01-405365 Park, 2016, CD19-targeted CAR T-cell therapeutics for hematologic malignancies: interpreting clinical outcomes to date, Blood, 127, 3312, 10.1182/blood-2016-02-629063 Johnson, 2017, Driving gene-engineered T cell immunotherapy of cancer, Cell Res, 27, 38, 10.1038/cr.2016.154 Maus, 2014, Antibody-modified T cells: CARs take the front seat for hematologic malignancies, Blood, 123, 2625, 10.1182/blood-2013-11-492231 Chen, 2015, CD19 chimeric antigen receptor T cell therapy for the treatment of B cell lineage acute lymphoblastic leukemia, Discov Med, 20, 185 Kalos, 2011, T cells with chimeric antigen receptors have potent antitumor effects and can establish memory in patients with advanced leukemia, Sci Transl Med, 3, 95ra73, 10.1126/scitranslmed.3002842 Almasbak, 2016, CAR T cell therapy: A game changer in cancer treatment, J Immunol Res, 2016, 5474602, 10.1155/2016/5474602 Yin, 2010, Detection and quantitation of bluetongue virus serotypes by a TaqMan probe-based real-time RT-PCR and differentiation from epizootic hemorrhagic disease virus, J Virol Methods, 168, 237, 10.1016/j.jviromet.2010.04.018 Cremonesi, 2014, Development of 23 individual TaqMan(R) real-time PCR assays for identifying common foodborne pathogens using a single set of amplification conditions, Food Microbiol, 43, 35, 10.1016/j.fm.2014.04.007 Latif, 2001, Fluorescence polarization in homogeneous nucleic acid analysis II: 5'-nuclease assay, Genome Res, 11, 436, 10.1101/gr.156601 Nagy, 2016, MeltMan: Optimization, Evaluation, and Universal Application of a qPCR System Integrating the TaqMan qPCR and Melting Analysis into a Single Assay, PLoS One, 11, e0151204, 10.1371/journal.pone.0151204 Afonina, 1997, Efficient priming of PCR with short oligonucleotides conjugated to a minor groove binder, Nucleic Acids Res, 25, 2657, 10.1093/nar/25.13.2657 Stamova, 2009, Identification and validation of suitable endogenous reference genes for gene expression studies in human peripheral blood, BMC Med Genomics, 2, 49, 10.1186/1755-8794-2-49 Zheng, 2012, Protein L: a novel reagent for the detection of chimeric antigen receptor (CAR) expression by flow cytometry, J Transl Med, 10, 29, 10.1186/1479-5876-10-29 De Oliveira, 2013, A CD19/Fc fusion protein for detection of anti-CD19 chimeric antigen receptors, J Transl Med, 11, 23, 10.1186/1479-5876-11-23 Emami-Shahri, 2016, Dynamic imaging for CAR-T-cell therapy, Biochem Soc Trans, 44, 386, 10.1042/BST20150257