Combining bacterial blight resistance and Basmati quality characteristics by phenotypic and molecular marker-assisted selection in rice
Tóm tắt
Bacterial Blight (BB) caused by Xanthomonas oryzae pv. oryzae is a major disease of rice in tropical Asia. Since all the Basmati varieties are highly susceptible and the disease is prevalent in the entire Basmati growing region of India, BB is a severe constraint in Basmati rice production. The present study was undertaken with the objective of combining the important Basmati quality traits with resistance to BB by a combination of phenotypic and molecular marker-assisted selection (MAS). Screening of 13 near-isogenic lines of rice against four isolates of the pathogen from Basmati growing regions identified the Xa4, xa8, xa13 and Xa21 effective against all the isolates tested. Two or more of these genes in combination imparted enhanced resistance as expressed by reduced average lesion length in comparison to individual genes. The two-gene pyramid line IRBB55 carrying xa13 and Xa21 was found equally effective as three/four gene pyramid lines. The two BB resistance genes present in IRBB55 were combined with the Basmati quality traits of Pusa Basmati-1 (PB-1), the most popular high yielding Basmati rice variety used as recurrent parent. Phenotypic selection for disease resistance, agronomic and Basmati quality characteristics and marker-assisted selection for the two resistance genes were carried out in BC1F1, BC1F2 and BC1F3 generations. Background analysis using 252 polymorphic amplified fragment length polymorphism (AFLP) markers detected 80.4 to 86.7% recurrent parent alleles in BC1F3 selections. Recombinants having enhanced resistance to BB, Basmati quality and desirable agronomic traits were identified, which can either be directly developed into commercial varieties or used as immediate donors of BB resistance in Basmati breeding programs.
Tài liệu tham khảo
Ahuja S. C., Panwar D. V. S., Ahuja U. and Gupta K. R. 1995. Bas-mati rice: The Scented Pearl. Directorate of Publication. Chaudhary Charan Singh Haryana Agricultural University, Hisar, India, pp. 13-24.
Brinda Priyadarsini V. and Gnanamanickam S. S. 1999. Occurrence of a subpopulation of Xanthomonas oryzae pv. oryzae with viru-lence to rice cultivar IRBB21 (Xa21) in Southern India. Plant Dis. 83: 781.
Chen S., Xu C. G., Lin X. H and Zhang Q. 2001. Improving bacte-rial blight resistance of '6078', an elite restorer line of hybrid rice, by molecular marker-aided selection. Plant Breed. 120: 133-137.
Dela Cruz N. and Khush G. S. 2000. Rice grain quality evaluation procedures. In: Singh R. K., Singh U. S. and Khush G. S. (eds), Aromatic Rices. Oxford & BH Publishing Co. Pvt. Ltd., New Delhi, India, pp. 16-28.
Doyle J. J. and Doyle J. L. 1990. Isolation of plant DNA from fresh tissues. Focus 12: 13-15.
Durgapal J. C. 1985. High virulence of Xanthomonas campestris pv. oryzae: A factor in the 1980 epiphytotic in the non-traditional rice growing regions of north west India. Ind. J. Agric. Sci. 55: 133-135.
Glaszmann J. C. 1987. Isoenzymes and classification of Asian rice varieties. Theor. Appl. Genet. 74: 21-30.
Huang N., Angeles E. R., Domingo J., Magpantay G., Singh S., Zhang G., Kumaravadivel N., Bennett J. and Khush G. S. 1997. Pyramiding of bacterial blight resistance genes in rice: marker assisted selection using RFLP and PCR. Theor. Appl. Genet. 95: 313-320.
Kauffman H. E., Reddy A. P. K., Hsieh S. P. Y. and Merca S. D. 1973. An improved technique for evaluating resistance of rice variet-ies to Xanthomonas oryzae. Plant Dis. Rep. 57: 537-541.
Khush G. S. and dela Cruz N. 2001. Developing Basmati rices with high yield potential. In: Duffy R. (ed.), Speciality Rices of the World-Breeding, Production and Marketing, FAO, Rome, Italy, pp. 15-18.
Kinoshita T. 1995. Report of committee on gene symbolization, nomenclature and linkage groups. Rice Genet. Newsl. 12: 9-153.
Lin X. H., Zhang D. P., Xie Y. F., Gao H. P. and Zhang Q. 1996. Identifying and mapping a new gene for bacterial blight resis-tance in rice based on RFLP markers. Phytopathology 86: 1156-1159.
Mew T. W., Vera Cruz C. M. and Medalla E. S. 1992. Changes in race frequency of Xanthomonas oryzae pv. oryzae in response to rice cultivars planted in the Philippines. Plant Dis. 76: 1029-1032.
Mundt C. C. 1990. Probability of mutation of multiple virulence and durability of resistance gene pyramids. Phytopathology 80 (3): 221-223.
Nagaraju J., Kathirvel M., Ramesh Kumar R., Siddiq E. A. and Ha-snain E. S. 2002. Genetic analysis of traditional and evolved bas-mati and non-basmati rice varieties by using fluorescence-based ISSR-PCR and SSR markers. Proc. Natl. Acad. Sci. USA 99: 5836-5841.
Nelson R. J., Ardales E. Y., Baraoidan M., Yap I., George M. L. C., Chen D. H., Finckh M., Bordeos A., Vera Cruz C. M., Adhikari T., Mundt C. C., Bustamam M., Cruz W., Leung H., Huang N., Yoshimura A., McCouch S. R., Mew T. W. and Leach J. E. 1996. Exploring the application of molecular markers for improving resistance to bacterial blight. In: Khush G. S. (ed.), Rice Genet-ics III. Proceedings of the Third International Rice Genetics Symposium, IRRI, Manila, Philippines, pp. 267-277.
Rohlf F. J. 1998. Numerical Taxonomy and Multivariate Analysis System (NTSYS-pc), ver. 2. 02f. Exeter Software, Setauket, New York, USA.
Ronald P. C., Albano B., Tabien R., Abenes L., Wu K., McCouch S. R. and Tanksley S. D. 1992. Genetic and physical analysis of the rice bacterial blight disease resistance locus, Xa21. Mol. Gen. Genet. 236: 113-120.
Sanchez A. C., Brar D. S., Huang N., Li Z. and Khush G. S. 2000. Sequence Tagged Site marker-assisted selection for three bacte-rial blight resistance genes in rice. Crop. Sci. 40: 792-797.
Singh S., Sidhu J. S., Huang N., Vikal Y., Li Z., Brar D. S., Dhali-wal H. S. and Khush G. S. 2001. Pyramiding three bacterial blight resistance genes (xa5, xa13 and Xa21) using marker-assisted se-lection into indica cultivar PR 106. Theor. Appl. Genet. 102: 1011-1015.
Singh V. P. 2000. The Basmati rice of India. In: Singh R. K., Singh U. S. and Khush G. S. (eds), Aromatic Rices. Oxford & IBH Pub-lishing Co. Pvt. Ltd., New Delhi, India, pp. 136-153.
Singh V. P., Siddiq E. A., Zaman F. U. and Sadananda A. R. 1988. Improved basmati donors. International Rice Res. Newsl. 13 (6): 22-25.
Sood B. C. and Siddiq E. A. 1978. A rapid technique for scent de-terminations in rice. Indian J. Genet. Plant Breed. 38: 2268-2271.
Vos P., Hogers R., Bleeker M., Reijans M., van de Lee T., Hornes M., Frijters A., Pot J., Peleman J., Kuiper M. and Zabeau M. 1995. AFLP: a new technique for DNA fingerprinting. Nucl. Ac-ids Res. 23: 4407-4414.
White F. F., Chittoor J. M., Leach J. E., Young S. A. and Zhu W. 1996. Molecular analysis of the interaction between Xanthomo-nas oryzae pv. oryzae and rice. In: Khush G. S. (ed.), Rice Ge-netics III. Proceedings of the Third International Rice Genetics Symposium, IRRI, Manila, Philippines, pp. 255-266.
Yoshimura S., Nelson R., Yoshimura A., Mew T. W. and Iwata N. 1992a. RFLP mapping of the bacterial blight resistance genes Xa-3 and Xa-4. Rice Genet. Newsl. 9: 136-138.
Yoshimura S., Yoshimura A., Saito A., Kishimoto N., Kawase M., Yano M., Nakagraha M., Ogawa T. and Iwata N. 1992b. RFLP analysis of introgressed segments in three near-isogenic lines of rice for bacterial blight resistance genes, Xa-1, Xa-3 and Xa-4. Jpn. J. Genet. 67: 29-37.
Yoshimura S., Yoshimura A., Iwata N., McCouch S. R., Abenes M. L., Baroidan M. R., Mew T. W. and Nelson R. J. 1995a. Tag-ging and combining bacterial blight resistance genes in rice us-ing RAPD and RFLP markers. Mol. Breed. 1: 375-387.
Yoshimura S., Yoshimura A., Nelson R. J., Mew T. W. and Iwata N. 1995b. Tagging Xa-1, the bacterial blight resistance gene in rice, by using RAPD markers. Jpn. J. Breed. 45: 81-85.
Zhang G., Angeles E. R., Abenes M. L. P., Huang N. and Khush G. S. 1996. RAPD and RFLP mapping of the bacterial blight resistance gene xa-13 in rice. Theor. Appl. Genet. 93: 65-70.