A high throughput method for enrichment of natural killer cells and lymphocytes and assessment of in vitro cytotoxicity

Beziat, 2011, CD56brightCD16+ NK cells: a functional intermediate stage of NK cell differentiation, J. Immunol., 186, 6753, 10.4049/jimmunol.1100330 Bjorkstrom, 2010, Expression patterns of NKG2A, KIR, and CD57 define a process of CD56dim NK-cell differentiation uncoupled from NK-cell education, Blood, 116, 3853, 10.1182/blood-2010-04-281675 Borella, 1995, The use of non-radioactive chromium as an alternative to 51Cr in NK assay, J. Immunol. Methods, 186, 101, 10.1016/0022-1759(95)00140-6 Busch, 2004, Isolation of peripheral blood CD4(+) T cells using RosetteSep and MACS for studies of DNA turnover by deuterium labeling, J.n Immunol. Methods, 286, 97, 10.1016/j.jim.2003.12.012 De Paoli, 1984, Enumeration of human lymphocyte subsets by monoclonal antibodies and flow cytometry: a comparative study using whole blood or mononuclear cells separated by density gradient centrifugation, J. Immunol. Methods, 72, 349, 10.1016/0022-1759(84)90003-6 English, 1974, Single-step separation of red blood cells. Granulocytes and mononuclear leukocytes on discontinuous density gradients of Ficoll-Hypaque, J. Immunol. Methods, 5, 249, 10.1016/0022-1759(74)90109-4 Gatti, 1998, Comparison of annexin V and calcein-AM as early vital markers of apoptosis in adherent cells by confocal laser microscopy, J. Histochem. Cytochem., 46, 895, 10.1177/002215549804600804 Grutzkau, 2010, Small but mighty: how the MACS-technology based on nanosized superparamagnetic particles has helped to analyze the immune system within the last 20years, Cytometry A, 77, 643, 10.1002/cyto.a.20918 Jang, 2012, An improved flow cytometry-based natural killer cytotoxicity assay involving calcein AM staining of effector cells, Ann. Clin. Lab. Sci., 42, 42 Kono, 2002, Impaired antibody-dependent cellular cytotoxicity mediated by herceptin in patients with gastric cancer, Cancer Res., 62, 5813 Levy, 2011, Natural killer cells in human cancer: from biological functions to clinical applications, J. Biomed. Biotechnol., 676198 Lichtenfels, 1994, CARE-LASS (calcein-release-assay), an improved fluorescence-based test system to measure cytotoxic T lymphocyte activity, J. Immunol. Methods, 172, 227, 10.1016/0022-1759(94)90110-4 Mansour, 1990, A rapid technique for lymphocyte preparation prior to two-color immunofluorescence analysis of lymphocyte subsets using flow cytometry. Comparison with density gradient separation, J. Immunol. Methods, 127, 61, 10.1016/0022-1759(90)90341-R Miltenyi, 1990, High gradient magnetic cell separation with MACS, Cytometry, 11, 231, 10.1002/cyto.990110203 Neri, 2001, Calcein-acetyoxymethyl cytotoxicity assay: standardization of a method allowing additional analyses on recovered effector cells and supernatants, Clin. Diagn. Lab. Immunol., 8, 1131, 10.1128/CDLI.8.6.1131-1135.2001 Nishimura, 1994, Protection against natural killer cells by interferon-gamma treatment of K562 cells cannot be explained by augmented major histocompatibility complex class I expression, Immunology, 83, 75 Palma, 2008, Evaluation of annexin V and calcein-AM as markers of mononuclear cell apoptosis during human immunodeficiency virus infection, Braz. J. Infect. Dis., 12, 108, 10.1590/S1413-86702008000200003 Roden, 1999, A novel cytolysis assay using fluorescent labeling and quantitative fluorescent scanning technology, J. Immunol. Methods, 226, 29, 10.1016/S0022-1759(99)00039-3 Sanchez-Correa, 2011, Human NK cells in acute myeloid leukaemia patients: analysis of NK cell-activating receptors and their ligands, Cancer Immunol. Immunother., 60, 1195, 10.1007/s00262-011-1050-2 Savary, 1979, Inhibition of murine natural killer cell-mediated cytotoxicity by pretreatment with ammonium chloride, J. Immunol. Methods, 25, 189, 10.1016/0022-1759(79)90055-3 Schlaeth, 2010, Fc-engineered EGF-R antibodies mediate improved antibody-dependent cellular cytotoxicity (ADCC) against KRAS-mutated tumor cells, Cancer Sci., 101, 1080, 10.1111/j.1349-7006.2010.01505.x Strome, 2007, A mechanistic perspective of monoclonal antibodies in cancer therapy beyond target-related effects, Oncologist, 12, 1084, 10.1634/theoncologist.12-9-1084 Uellner, 1997, Perforin is activated by a proteolytic cleavage during biosynthesis which reveals a phospholipid-binding C2 domain, EMBO J., 16, 7287, 10.1093/emboj/16.24.7287 Watanabe, 2010, NK cell dysfunction with down-regulated CD16 and up-regulated CD56 molecules in patients with esophageal squamous cell carcinoma, Dis. Esophagus., 23, 675, 10.1111/j.1442-2050.2010.01073.x Yokoyama, 2006, Licensing of natural killer cells by self-major histocompatibility complex class I, Immunol. Rev., 214, 143, 10.1111/j.1600-065X.2006.00458.x